Note to users. If you're seeing this message, it means that your browser cannot find this page's style/presentation instructions -- or possibly that you are using a browser that does not support current Web standards. Find out more about why this message is appearing, and what you can do to make your experience of our site the best it can be.

Site Tools

  • AAAS
  • Subscribe
  • Feedback

Site Search

Search Advanced

Science 7 January 1994:
Vol. 263. no. 5143, pp. 95 - 98
DOI: 10.1126/science.8272874
Prev | Table of Contents | Next

Articles

Science, Vol 263, Issue 5143, 95-98
Copyright © 1994 by American Association for the Advancement of Science

articles

Targeting of G alpha i2 to the Golgi by alternative spliced carboxyl-terminal region

JP Montmayeur and E Borrelli

Laboratoire de Genetique Moleculaire des Eucaryotes, CNRS, INSERM U184, Faculte de Medecine, Strasbourg, France.

Heterotrimeric guanosine triphosphate (GTP)-binding proteins (G proteins) may participate in membrane traffic events. A complementary DNA (cDNA) was isolated from a mouse pituitary cDNA library that corresponded to an alternatively spliced form of the gene encoding the G protein alpha subunit G alpha i2. The cDNA was identical to that encoding G alpha i2 except that the region encoding for the carboxyl-terminal 24 amino acids was replaced by a longer region encoding 35 amino acids that have no sequence similarity with G alpha i2 or other members of the G protein family. This alternative spliced product and the corresponding protein (sGi2) were present in several tissues. Specific antibodies revealed that sGi2 was localized in the Golgi apparatus, suggesting a role in membrane transport. Thus, alternative splicing may generate from a single gene two G protein alpha subunits with differential cellular localization and function.


THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES:
Role of a G{alpha}i2 protein splice variant in the formation of an intracellular dopamine D2 receptor pool.
M. F. Lopez-Aranda, M. J. Acevedo, A. Gutierrez, P. Koulen, and Z. U. Khan (2007)
J. Cell Sci. 120, 2171-2178
   Abstract »    Full Text »    PDF »
Dexras1/AGS-1, a Steroid Hormone-Induced Guanosine Triphosphate-Binding Protein, Inhibits 3',5'-Cyclic Adenosine Monophosphate-Stimulated Secretion in AtT-20 Corticotroph Cells.
T. E. Graham, T. A. Key, K. Kilpatrick, and R. I. Dorin (2001)
Endocrinology 142, 2631-2640
   Abstract »    Full Text »    PDF »
Role of Alternative Splicing in Generating Isoform Diversity Among Plasma Membrane Calcium Pumps.
E. E. Strehler and D. A. Zacharias (2001)
Physiol Rev 81, 21-50
   Abstract »    Full Text »    PDF »
A Proline-rich Region and Nearby Cysteine Residues Target XLalpha s to the Golgi Complex Region.
O. Ugur and T. L. Z. Jones (2000)
Mol. Biol. Cell 11, 1421-1432
   Abstract »    Full Text »
GERp95, a Membrane-associated Protein that Belongs to a Family of Proteins Involved in Stem Cell Differentiation.
D. E. Cikaluk, N. Tahbaz, L. C. Hendricks, G. E. DiMattia, D. Hansen, D. Pilgrim, and T. C. Hobman (1999)
Mol. Biol. Cell 10, 3357-3372
   Abstract »    Full Text »
Trimeric G proteins modulate the dynamic interaction of PKAII with the Golgi complex.
M. Martin, J Hidalgo, F. Vega, and A Velasco (1999)
J. Cell Sci. 112, 3869-3878
   Abstract »    PDF »
Characterization of the Major Bovine Brain Go alpha  Isoforms. MAPPING THE STRUCTURAL DIFFERENCES BETWEEN THE alpha  SUBUNIT ISOFORMS IDENTIFIES A VARIABLE REGION OF THE PROTEIN INVOLVED IN RECEPTOR INTERACTIONS.
W. E. McIntire, J. Dingus, K. L. Schey, and J. D. Hildebrandt (1998)
J. Biol. Chem. 273, 33135-33141
   Abstract »    Full Text »    PDF »
In Vitro Reconstitution of Microtubule Plus End-directed, GTPgamma S-sensitive Motility ofGolgi Membranes.
A. T. Fullerton, M.-Y. Bau, P. A. Conrad, and G. S. Bloom (1998)
Mol. Biol. Cell 9, 2699-2714
   Abstract »    Full Text »
Differential Distribution of Dynamin Isoforms in Mammalian Cells.
H. Cao, F. Garcia, and M. A. McNiven (1998)
Mol. Biol. Cell 9, 2595-2609
   Abstract »    Full Text »
A Regulatory Role for cAMP-dependent Protein Kinase in Protein Traffic along the Exocytic Route.
M. Muniz, M. Alonso, J. Hidalgo, and A. Velasco (1996)
J. Biol. Chem. 271, 30935-30941
   Abstract »    Full Text »    PDF »
The Heterotrimeric G-protein G[IMAGE] Is Localized to the Insulin Secretory Granules of [IMAGE]-Cells and Is Involved in Insulin Exocytosis.
R. J. Konrad, R. A. Young, R. D. Record, R. M. Smith, P. Butkerait, D. Manning, L. Jarett, and B. A. Wolf (1995)
J. Biol. Chem. 270, 12869-12876
   Abstract »    Full Text »    PDF »


To Advertise     Find Products

Science. ISSN 0036-8075 (print), 1095-9203 (online)