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Science 24 December 1993:
Vol. 262. no. 5142, pp. 2033 - 2035
DOI: 10.1126/science.8266099
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Articles

Science, Vol 262, Issue 5142, 2033-2035
Copyright © 1993 by American Association for the Advancement of Science

articles

Chromosome condensation in Xenopus mitotic extracts without histone H1

K Ohsumi, C Katagiri, and T Kishimoto

Laboratory of Cell and Developmental Biology, Faculty of Biosciences, Tokyo Institute of Technology, Japan.

The contribution of histone H1 to mitotic chromosome condensation was examined with the use of a cell-free extract from Xenopus eggs, which transforms condensed sperm nuclei into metaphase chromosomes. When H1 was removed from the extract, the resultant metaphase chromosomes were indistinguishable from those formed in complete extract. Nucleosomal spacing was the same for both. Thus, H1 is not required for the structural reorganization that leads to condensed metaphase chromosomes in this egg extract.


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Histone H1 is essential for mitotic chromosome architecture and segregation in Xenopus laevis egg extracts.
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J. Biol. Chem. 274, 14533-14536
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Phosphorylation of histone H3 at serine 10 is correlated with chromosome condensation during mitosis and meiosis in Tetrahymena.
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Corequirement of Specific Phosphoinositides and Small GTP-binding Protein Cdc42 in Inducing Actin Assembly in Xenopus Egg Extracts.
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Phosphorylation of Linker Histones by a Protein Kinase A-like Activity in Mitotic Nuclei.
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Increased Phosphorylation of Histone H1 in Mouse Fibroblasts Transformed with Oncogenes or Constitutively Active Mitogen-activated Protein Kinase Kinase.
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SMC2, a Saccharomyces cerevisiae gene essential for chromosome segregation and condensation, defines a subgroup within the SMC family..
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Meiosis-specific cell cycle regulation in maturing Xenopus oocytes.
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Science. ISSN 0036-8075 (print), 1095-9203 (online)