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Science 10 December 1993:
Vol. 262. no. 5140, pp. 1737 - 1741
DOI: 10.1126/science.7505062
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Articles

Science, Vol 262, Issue 5140, 1737-1741
Copyright © 1993 by American Association for the Advancement of Science

articles

Abnormal chromosome behavior in Neurospora mutants defective in DNA methylation

HM Foss, CJ Roberts, KM Claeys, and EU Selker

Institute of Molecular Biology, University of Oregon, Eugene 97403.

The function and regulation of DNA methylation in eukaryotes remain unclear. Genes affecting methylation were identified in the fungus Neurospora crassa. A mutation in one gene, dim-2, resulted in the loss of all detectable DNA methylation. Abnormal segregation of the methylation defects in crosses led to the discovery that the methylation mutants frequently generate strains with extra chromosomes or chromosomal parts. Starvation for S-adenosylmethionine, the presumed methyl group donor for DNA methylation, also produced aneuploidy. These results suggest that DNA methylation plays a role in the normal control of chromosome behavior.


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DNA methylation inhibits elongation but not initiation of transcription in Neurospora crassa.
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