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Science 10 December 1993:
Vol. 262. no. 5140, pp. 1724 - 1728
DOI: 10.1126/science.8259516
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Articles

Science, Vol 262, Issue 5140, 1724-1728
Copyright © 1993 by American Association for the Advancement of Science

articles

Dense nonsymmetrical DNA methylation resulting from repeat-induced point mutation in Neurospora

EU Selker, DY Fritz, and MJ Singer

Institute of Molecular Biology, University of Oregon, Eugene 97403.

Cytosine methylation has been implicated in epigenetic control of gene expression in animals, plants, and fungi. It has been assumed that all methylation in eukaryotes is at symmetrical sequences such as CpG/GpC, because this can explain perpetuation of methylation states. Here the bisulfite genomic sequencing method was used to examine methylation in DNA from a Neurospora gene exposed to repeat-induced point mutation. 5-Methylcytosine was not limited to symmetrical sites and individual molecules showed different patterns and amounts of modification. The methylation extended beyond the mutated region and even beyond the edge of the duplicated segment.


THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES:
Relics of repeat-induced point mutation direct heterochromatin formation in Neurospora crassa.
Z. A. Lewis, S. Honda, T. K. Khlafallah, J. K. Jeffress, M. Freitag, F. Mohn, D. Schubeler, and E. U. Selker (2009)
Genome Res. 19, 427-437
   Abstract »    Full Text »    PDF »
Genome Defense and DNA Methylation in Neurospora.
E.U. SELKER (2004)
Cold Spring Harb Symp Quant Biol 69, 119-124
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Eukaryotic Methyl-CpG-Binding Domain Proteins and Chromatin Modification.
M.-S. Hung and C.-K. J. Shen (2003)
Eukaryot. Cell 2, 841-846
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Synthesis of Signals for De Novo DNA Methylation in Neurospora crassa.
H. Tamaru and E. U. Selker (2003)
Mol. Cell. Biol. 23, 2379-2394
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Identification and Characterization of the Genes Encoding the Core Histones and Histone Variants of Neurospora crassa.
S. M. Hays, J. Swanson, and E. U. Selker (2002)
Genetics 160, 961-973
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Arabidopsis cmt3 chromomethylase mutations block non-CG methylation and silencing of an endogenous gene.
L. Bartee, F. Malagnac, and J. Bender (2001)
Genes & Dev. 15, 1753-1758
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Bisulfite genomic sequencing: systematic investigation of critical experimental parameters.
C. Grunau, S. J. Clark, and A. Rosenthal (2001)
Nucleic Acids Res. 29, e65
   Abstract »    Full Text »    PDF »
Methylation is not the main force repressing the retrotransposon MAGGY in Magnaporthe grisea.
H. Nakayashiki, K. Ikeda, Y. Hashimoto, Y. Tosa, and S. Mayama (2001)
Nucleic Acids Res. 29, 1278-1284
   Abstract »    Full Text »    PDF »
Action of Repeat-Induced Point Mutation on Both Strands of a Duplex and on Tandem Duplications of Various Sizes in Neurospora.
M. K. Watters, T. A. Randall, B. S. Margolin, E. U. Selker, and D. R. Stadler (1999)
Genetics 153, 705-714
   Abstract »    Full Text »    PDF »
Polymorphisms and Genomic Organization of Repetitive DNA from Centromeric Regions of Arabidopsis Chromosomes.
J. S. Heslop-Harrison, M. Murata, Y. Ogura, T. Schwarzacher, and F. Motoyoshi (1999)
PLANT CELL 11, 31-42
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Trichostatin A causes selective loss of DNA methylation in Neurospora.
E. U. Selker (1998)
PNAS 95, 9430-9435
   Abstract »    Full Text »    PDF »
Methylation of the minimal promoter of an embryonic globin gene silences transcription in primary erythroid cells.
R. Singal, R. Ferris, J. A. Little, S. Z. Wang, and G. D. Ginder (1997)
PNAS 94, 13724-13729
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DNA methylation inhibits elongation but not initiation of transcription in Neurospora crassa.
M. R. Rountree and E. U. Selker (1997)
Genes & Dev. 11, 2383-2395
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Analysis of the Methylation Pattern of the Maize Opaque-2 (O2) Promoter and in Vitro Binding Studies Indicate That the O2 B-Zip Protein and Other Endosperm Factors Can Bind to Methylated Target Sequences.
V. Rossi, M. Motto, and L. Pellegrini (1997)
J. Biol. Chem. 272, 13758-13765
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Absence of an Unusual "Densely Methylated Island" at the Hamster dhfr ori-beta.
T. Rein, D. A. Natale, U. Gartner, M. Niggemann, M. L. DePamphilis, and H. Zorbas (1997)
J. Biol. Chem. 272, 10021-10029
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Mammalian DNA (Cytosine-5-)-methyltransferase Expressed in Escherichia coli, Purified and Characterized.
T. O. Tollefsbol and C. A. Hutchison , III (1995)
J. Biol. Chem. 270, 18543-18550
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Developmental control of allelic methylation in the imprinted mouse Igf2 and H19 genes.
R Feil, J Walter, N. Allen, and W Reik (1994)
Development 120, 2933-2943
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Dissection of the Signal for DNA Methylation in the {zeta}-{eta} Region of Neurospora.
E.U. Selker, G.A. Richardson, P.W. Garrett-Engele, M.J. Singer, and V. Miao (1993)
Cold Spring Harb Symp Quant Biol 58, 323-329
   Abstract »    PDF »


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