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Science 10 December 1993:
Vol. 262. no. 5140, pp. 1721 - 1724
DOI: 10.1126/science.7903123
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Articles
Science, Vol 262, Issue 5140, 1721-1724
Copyright © 1993 by American Association for the Advancement of Science
Restoration of HIV-specific cell-mediated immune responses by interleukin-12 in vitro
M Clerici,
DR Lucey,
JA Berzofsky,
LA Pinto,
TA Wynn,
SP Blatt,
MJ Dolan,
CW Hendrix,
SF Wolf,
and
GM Shearer
Experimental Immunology Branch, National Cancer Institute (NCI), National Institutes of Health (NIH), Bethesda, MD 20892.
Peripheral blood mononuclear cells (PBMCs) from many asymptomatic individuals infected with human immunodeficiency virus-type 1 (HIV) are unresponsive as measured by in vitro T cell proliferation and interleukin-2 (IL-2) production to influenza virus and synthetic peptides of HIV envelope (Env). Strong influenza virus- and Env-stimulated IL-2 responses and T cell proliferation were restored when cultures were stimulated in the presence of IL-12. Interferon-gamma production by PBMCs from HIV seropositive (HIV+) patients was also restored with IL-12. Furthermore, in vitro antigen-specific production of IL-2 and proliferation of PBMCs from HIV- donors were suppressed by antibody to IL-12, but were not enhanced by addition of exogenous IL-12. Thus, IL-12 may be limiting in PBMCs from HIV+ but not HIV- individuals. These findings demonstrate that IL-12 can restore HIV-specific cell-mediated immunity in vitro in HIV-infected individuals and suggest a potential use of IL-12 in augmenting the diminished immunologic functions associated with HIV infection.
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