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Science 3 December 1993: Vol. 262. no. 5139, pp. 1572 - 1575 DOI: 10.1126/science.8248807
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Articles
Science, Vol 262, Issue 5139, 1572-1575
Copyright © 1993 by American Association for the Advancement of Science
A link between cyclin A expression and adhesion-dependent cell cycle progression
TM Guadagno,
M Ohtsubo,
JM Roberts,
and
RK Assoian
Department of Biochemistry and Molecular Biophysics, Columbia University, New York, NY 10032.
Cell adhesion has an essential role in regulating proliferation during the G1 phase of the cell cycle, and loss of this adhesion requirement is a classic feature of oncogenic transformation. The appearance of cyclin A messenger RNA and protein in late G1 was dependent on cell adhesion in both NRK and NIH 3T3 fibroblasts. In contrast, the expression of Cdc2, Cdk2, cyclin D1, and cyclin E was independent of adhesion in both cell lines. Transfection of NRK cells with a cyclin A complementary DNA resulted in adhesion-independent accumulation of cyclin A protein and cyclin A-associated kinase activity. These transfected cells also entered S phase and complete multiple rounds of cell division in the absence of cell adhesion. Thus, cyclin A is a target of the adhesion-dependent signals that control cell proliferation.
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