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Science 3 December 1993:
Vol. 262. no. 5139, pp. 1569 - 1572
DOI: 10.1126/science.7504324
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Articles

Science, Vol 262, Issue 5139, 1569-1572
Copyright © 1993 by American Association for the Advancement of Science

articles

Activation of exocytosis by the heterotrimeric G protein Gi3

M Aridor, G Rajmilevich, MA Beaven, and R Sagi-Eisenberg

Weizmann Institute of Science, Department of Chemical Immunology, Rehovot, Israel.

Secretagogues of rat peritoneal mast cells, such as mastoparan and compound 48/80, induce mast cell exocytosis by activating directly the guanosine triphosphate-binding proteins that are required for exocytosis. The introduction of a synthetic peptide that corresponds to the carboxyl-terminal end sequence of G alpha i3 into the cells specifically blocked this secretion. Similar results were obtained when antibodies to this peptide were introduced. The G alpha i3 was located in both the Golgi and the plasma membrane, but only the latter source of G alpha i3 appeared to be essential for secretion. These results indicate that G alpha i3 functions to control regulated exocytosis in mast cells.


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Science. ISSN 0036-8075 (print), 1095-9203 (online)