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Science 3 December 1993:
Vol. 262. no. 5139, pp. 1563 - 1566
DOI: 10.1126/science.8248805
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Articles

Science, Vol 262, Issue 5139, 1563-1566
Copyright © 1993 by American Association for the Advancement of Science

articles

Nucleosome disruption by transcription factor binding in yeast

RH Morse

Laboratory of Cellular and Developmental Biology, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Bethesda, MD 20892.

Studies in vivo and in vitro have shown that the packaging of DNA into chromatin can affect gene expression. Here, binding of the yeast transcriptional activator GAL4 to DNA in chromatin has been investigated in vivo with a yeast episome. A positioned nucleosome that is present in cells grown in glucose and contains a single GAL4 binding site is disrupted by GAL4 binding in galactose. GAL4 can also bind to DNA in chromatin when the carboxyl-terminal activation domain of GAL4 is either masked by GAL80 or is absent. These results show that a transcription factor can bind to its site in vivo in what would appear to be a repressive chromatin structure.


THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES:
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Nucleosome displacement in transcription.
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GCN5 Dependence of Chromatin Remodeling and Transcriptional Activation by the GAL4 and VP16 Activation Domains in Budding Yeast.
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Artificially Recruited TATA-Binding Protein Fails To Remodel Chromatin and Does Not Activate Three Promoters That Require Chromatin Remodeling.
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Chromatin Opening and Transactivator Potentiation by RAP1 in Saccharomyces cerevisiae.
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Sequence Selectivity of c-Myb in Vivo. RESOLUTION OF A DNA TARGET SPECIFICITY PARADOX.
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Binding of Gal4p and Bicoid to Nucleosomal Sites in Yeast in the Absence of Replication.
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Promoter Structure and Transcriptional Activation with Chromatin Templates Assembled In Vitro. A SINGLE Gal4-VP16 DIMER BINDS TO CHROMATIN OR TO DNA WITH COMPARABLE AFFINITY.
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SWI-SNF Complex Participation in Transcriptional Activation at a Step Subsequent to Activator Binding.
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Chromatin Remodeling by Transcriptional Activation Domains in a Yeast Episome.
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ATP-dependent nucleosome reconfiguration and transcriptional activation from preassembled chromatin templates.
M. Pazin, R. Kamakaka, and J. Kadonaga (1994)
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Increased recruitment of TATA-binding protein to the promoter by transcriptional activation domains in vivo.
C Klein and K Struhl (1994)
Science 266, 280-282
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Stimulation of GAL4 derivative binding to nucleosomal DNA by the yeast SWI/SNF complex.
J Cote, J Quinn, J. Workman, and C. Peterson (1994)
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The global transcriptional regulators, SSN6 and TUP1, play distinct roles in the establishment of a repressive chromatin structure..
J P Cooper, S Y Roth, and R T Simpson (1994)
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The N-terminal and C-terminal Domains of RAP1 Are Dispensable for Chromatin Opening and GCN4-mediated HIS4 Activation in Budding Yeast.
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