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Science 26 November 1993:
Vol. 262. no. 5138, pp. 1444 - 1448
DOI: 10.1126/science.8248783
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Articles

Science, Vol 262, Issue 5138, 1444-1448
Copyright © 1993 by American Association for the Advancement of Science

articles

Phosphatidylinositol 4-kinase: gene structure and requirement for yeast cell viability

CA Flanagan, EA Schnieders, AW Emerick, R Kunisawa, A Admon, and J Thorner

Department of Molecular and Cell Biology, University of California, Berkeley 94720.

Phosphatidylinositol (PtdIns) 4-kinase catalyzes the first step in the biosynthesis of PtdIns-4,5-bisphosphate (PtdIns[4,5]P2). Hydrolysis of PtdIns[4,5]P2 in response to extracellular stimuli is thought to initiate intracellular signaling cascades that modulate cell proliferation and differentiation. The PIK1 gene encoding a PtdIns 4-kinase from the yeast Saccharomyces cerevisiae was isolated by polymerase chain reaction (PCR) with oligonucleotides based on the sequence of peptides derived from the purified enzyme. The sequence of the PIK1 gene product bears similarities to that of PtdIns 3-kinases from mammals (p110) and yeast (Vps34p). Expression of PIK1 from a multicopy plasmid elevated PtdIns 4-kinase activity and enhanced the response to mating pheromone. A pik1 null mutant was inviable, indicating that PtdIns4P and presumably PtdIns[4,5]P2 are indispensable phospholipids.


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