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Science 19 November 1993:
Vol. 262. no. 5137, pp. 1255 - 1257
DOI: 10.1126/science.7694369
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Articles

Science, Vol 262, Issue 5137, 1255-1257
Copyright © 1993 by American Association for the Advancement of Science

articles

Photocrosslinking of 5-iodouracil-substituted RNA and DNA to proteins

MC Willis, BJ Hicke, OC Uhlenbeck, TR Cech, and TH Koch

Department of Chemistry and Biochemistry, University of Colorado, Boulder 80309.

5-Iodouracil-substituted RNA and DNA were crosslinked regiospecifically to associated proteins in yields of 70 to 94% of bound nucleic acid. Irradiation of the iodouracil chromophore with monochromatic, long-wavelength ultraviolet radiation (325 nanometers) eliminates excitation of other nucleic acid and protein chromophores. The combination of high crosslinking yields, excellent specificity, and elimination of photodamage to other chromophores represents an important advance toward the precise identification of contacts in nucleoprotein complexes.


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