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Science 12 November 1993:
Vol. 262. no. 5136, pp. 1051 - 1054
DOI: 10.1126/science.8235622
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Articles

Science, Vol 262, Issue 5136, 1051-1054
Copyright © 1993 by American Association for the Advancement of Science

articles

Release of active cytokinin by a beta-glucosidase localized to the maize root meristem

B Brzobohaty, I Moore, P Kristoffersen, L Bako, N Campos, J Schell, and K Palme

Max-Planck Institut fur Zuchtungsforschung, Koln Federal Republic of Germany.

A beta-glucoside encoded by a cloned Zea mays complementary DNA (Zm-p60.1) cleaved the biologically inactive hormone conjugates cytokinin-O-glucosides and kinetin-N3-glucoside, releasing active cytokinin. Tobacco protoplasts that transiently expressed Zm-p60.1 could use the inactive cytokinin glucosides to initiate cell division. The ability of protoplasts to sustain growth in response to cytokinin glucosides persisted indefinitely after the likely disappearance of the expression vector. In the roots of maize seedlings, Zm-p60.1 was localized to the meristematic cells and may function in vivo to supply the developing maize embryo with active cytokinin.


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