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Science 12 November 1993:
Vol. 262. no. 5136, pp. 1048 - 1050
DOI: 10.1126/science.8235621
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Articles

Science, Vol 262, Issue 5136, 1048-1050
Copyright © 1993 by American Association for the Advancement of Science

articles

Replication-specific inactivation of the pT181 plasmid initiator protein

A Rasooly and RP Novick

Department of Plasmid Biology, Public Health Research Institute, New York, NY 10016.

Replication of the Staphylococcus aureus plasmid pT181, which occurs by the rolling circle mechanism, is accompanied by the covalent attachment of a approximately 12-residue oligodeoxy-nucleotide to one subunit of the dimeric plasmid-coded initiator protein, RepC. This oligonucleotide represents the plasmid sequence immediately 3' to the initiating nick site. The resulting heterodimeric protein lacks the topoisomerase and replication activities of unmodified RepC, suggesting that the regulation of plasmid DNA replication requires post-replicational inactivation of the initiator protein as well as control of its synthesis.


THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES:
Profile of Richard P. Novick.
M. Marino (2007)
PNAS 104, 14179-14181
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Transcriptional inactivation of a regulatory site for replication of Vibrio cholerae chromosome II.
T. Venkova-Canova, P. Srivastava, and D. K. Chattoraj (2006)
PNAS 103, 12051-12056
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Multipartite Regulation of rctB, the Replication Initiator Gene of Vibrio cholerae Chromosome II.
D. Pal, T. Venkova-Canova, P. Srivastava, and D. K. Chattoraj (2005)
J. Bacteriol. 187, 7167-7175
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Role of Individual Monomers of a Dimeric Initiator Protein in the Initiation and Termination of Plasmid Rolling Circle Replication.
T.-L. Chang, M. G. Kramer, R. A. Ansari, and S. A. Khan (2000)
J. Biol. Chem. 275, 13529-13534
   Abstract »    Full Text »    PDF »
An Oligonucleotide Inhibits Oligomerization of a Rolling Circle Initiator Protein at the pT181 Origin of Replication.
A. C. Zhao, R. A. Ansari, M. C. Schmidt, and S. A. Khan (1998)
J. Biol. Chem. 273, 16082-16089
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Replication and Control of Circular Bacterial Plasmids.
G. del Solar, R. Giraldo, M. J. Ruiz-Echevarria, M. Espinosa, and R. Diaz-Orejas (1998)
Microbiol. Mol. Biol. Rev. 62, 434-464
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The Inactive pT181 Initiator Heterodimer, RepC/C, Binds but Fails to Induce Melting of the Plasmid Replication Origin.
R. Jin, X. Zhou, and R. P. Novick (1996)
J. Biol. Chem. 271, 31086-31091
   Abstract »    Full Text »    PDF »
Specific Nicking-Closing Activity of the Initiator of Replication Protein RepB of Plasmid pMV158 on Supercoiled or Single-stranded DNA.
M. Moscoso, G. del Solar, and M. Espinosa (1995)
J. Biol. Chem. 270, 3772-3779
   Abstract »    Full Text »    PDF »


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