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Science 22 October 1993:
Vol. 262. no. 5133, pp. 575 - 578
DOI: 10.1126/science.8211185
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Articles

Science, Vol 262, Issue 5133, 575-578
Copyright © 1993 by American Association for the Advancement of Science

articles

Potentiation by the beta subunit of the ratio of the ionic current to the charge movement in the cardiac calcium channel

A Neely, X Wei, R Olcese, L Birnbaumer, and E Stefani

Department of Molecular Physiology and Biophysics, Baylor College of Medicine, Houston, TX 77030.

The voltage-activated rabbit cardiac calcium channel alpha 1 subunit was expressed in Xenopus oocytes. The charge movement of its voltage sensor was measured and related to the opening of the ion-conducting pore. The half-activation potential for charge movement was 35 millivolts more negative than that for pore opening. Coexpression of the cardiac calcium channel beta subunit reduced this difference without affecting charge movement. Thus, intramolecular coupling between the voltage sensor and the channel pore opening can be facilitated by a regulatory subunit.


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