Note to users. If you're seeing this message, it means that your browser cannot find this page's style/presentation instructions -- or possibly that you are using a browser that does not support current Web standards. Find out more about why this message is appearing, and what you can do to make your experience of our site the best it can be.

Site Tools

  • AAAS
  • Subscribe
  • Feedback

Site Search

Search Advanced

Science 1 October 1993:
Vol. 262. no. 5130, pp. 105 - 108
DOI: 10.1126/science.8211113
Prev | Table of Contents | Next

Articles

Science, Vol 262, Issue 5130, 105-108
Copyright © 1993 by American Association for the Advancement of Science

articles

Correspondence between a mammalian spliceosome component and an essential yeast splicing factor

M Bennett and R Reed

Department of Cell Biology, Harvard Medical School, Boston, MA 02115.

None of the mammalian splicing factors that have been cloned corresponds to the yeast pre-messenger RNA splicing factors, the PRP proteins. Here, a generalizable strategy was used to isolate a complementary DNA encoding the mammalian spliceosome-associated protein (SAP) SAP 62. It is demonstrated that SAP 62 is the likely functional homolog of the yeast PRP11 protein. Both PRP11 and SAP 62 associate stably with the spliceosome, contain a single zinc finger, and display significant amino acid sequence similarity. Unlike PRP11, SAP 62 contains 22 proline-rich heptapeptide repeats at the carboxyl-terminus.


THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES:
DNA sequence variations of the entire anti-Mullerian hormone (AMH) gene promoter and AMH protein expression in patients with the Mayer-Rokitanski-Kuster-Hauser syndrome.
P. Oppelt, P.L. Strissel, A. Kellermann, S. Seeber, A. Humeny, M.W. Beckmann, and R. Strick (2005)
Hum. Reprod. 20, 149-157
   Abstract »    Full Text »    PDF »
Human RNPS1 and Its Associated Factors: a Versatile Alternative Pre-mRNA Splicing Regulator In Vivo.
E. Sakashita, S. Tatsumi, D. Werner, H. Endo, and A. Mayeda (2004)
Mol. Cell. Biol. 24, 1174-1187
   Abstract »    Full Text »    PDF »
Domains in Human Splicing Factors SF3a60 and SF3a66 Required for Binding to SF3a120, Assembly of the 17S U2 snRNP, and Prespliceosome Formation.
D. Nesic and A. Kramer (2001)
Mol. Cell. Biol. 21, 6406-6417
   Abstract »    Full Text »    PDF »
Sequences upstream of the branch site are required to form helix II between U2 and U6 snRNA in a trans-splicing reaction.
G. Ast, T. Pavelitz, and A. M. Weiner (2001)
Nucleic Acids Res. 29, 1741-1749
   Abstract »    Full Text »    PDF »
Characterization of a Protein Complex Containing Spliceosomal Proteins SAPs 49, 130, 145, and 155.
B. K. Das, L. Xia, L. Palandjian, O. Gozani, Y. Chyung, and R. Reed (1999)
Mol. Cell. Biol. 19, 6796-6802
   Abstract »    Full Text »    PDF »
Detection of a Novel ATP-Dependent Cross-Linked Protein at the 5' Splice Site-U1 Small Nuclear RNA Duplex by Methylene Blue-Mediated Photo-Cross-Linking.
Z.-R. Liu, B. Sargueil, and C. W. J. Smith (1998)
Mol. Cell. Biol. 18, 6910-6920
   Abstract »    Full Text »
CUS2, a Yeast Homolog of Human Tat-SF1, Rescues Function of Misfolded U2 through an Unusual RNA Recognition Motif.
D. Yan, R. Perriman, H. Igel, K. J. Howe, M. Neville, and M. Ares Jr. (1998)
Mol. Cell. Biol. 18, 5000-5009
   Abstract »    Full Text »
WW domain-mediated interactions reveal a spliceosome-associated protein that binds a third class of proline-rich motif: The proline glycine and methionine-rich motif.
M. T. Bedford, R. Reed, and P. Leder (1998)
PNAS 95, 10602-10607
   Abstract »    Full Text »    PDF »
A Potential Role for U2AF-SAP 155 Interactions in Recruiting U2 snRNP to the Branch Site.
O. Gozani, J. Potashkin, and R. Reed (1998)
Mol. Cell. Biol. 18, 4752-4760
   Abstract »    Full Text »
Phosphorylation of spliceosomal protein SAP 155 coupled with splicing catalysis.
C. Wang, K. Chua, W. Seghezzi, E. Lees, O. Gozani, and R. Reed (1998)
Genes & Dev. 12, 1409-1414
   Abstract »    Full Text »
Multiple Developmental Requirements of Noisette, the Drosophila Homolog of the U2 snRNP-Associated Polypeptide SF3a60.
V. Meyer, B. Oliver, and D. Pauli (1998)
Mol. Cell. Biol. 18, 1835-1843
   Abstract »    Full Text »
KIF3C and KIF3A Form a Novel Neuronal Heteromeric Kinesin That Associates with Membrane Vesicles.
V. Muresan, T. Abramson, A. Lyass, D. Winter, E. Porro, F. Hong, N. L. Chamberlin, and B. J. Schnapp (1998)
Mol. Biol. Cell 9, 637-652
   Abstract »    Full Text »
Molecular Characterization of a Novel, Widespread Nuclear Protein That Colocalizes with Spliceosome Components.
M. S. Schmidt-Zachmann, S. Knecht, and A. Krämer (1998)
Mol. Biol. Cell 9, 143-160
   Abstract »    Full Text »
Cloning of mDEAH9, a putative RNA helicase and mammalian homologue of Saccharomyces cerevisiae splicing factor Prp43.
S. Gee, S. W. Krauss, E. Miller, K. Aoyagi, J. Arenas, and J. G. Conboy (1997)
PNAS 94, 11803-11807
   Abstract »    Full Text »    PDF »
U2AF65 recruits a novel human DEAD box protein required for the U2 snRNP-branchpoint interaction..
J Fleckner, M Zhang, J Valcarcel, and M R Green (1997)
Genes & Dev. 11, 1864-1872
   Abstract »    PDF »
Dynamics of the U1 Small Nuclear Ribonucleoprotein during Yeast Spliceosome Assembly.
S. W. Ruby (1997)
J. Biol. Chem. 272, 17333-17341
   Abstract »    Full Text »    PDF »
The nuclear receptor SF-1 mediates sexually dimorphic expression of Mullerian Inhibiting Substance, in vivo.
G Giuili, W. Shen, and H. Ingraham (1997)
Development 124, 1799-1807
   Abstract »    PDF »
A human protein required for the second step of pre-mRNA splicing is functionally related to a yeast splicing factor..
D S Horowitz and A R Krainer (1997)
Genes & Dev. 11, 139-151
   Abstract »    PDF »
The Saccharomyces cerevisiae Prp5 Protein Has RNA-dependent ATPase Activity with Specificity for U2 Small Nuclear RNA.
C. L. O'Day, G. Dalbadie-McFarland, and J. Abelson (1996)
J. Biol. Chem. 271, 33261-33267
   Abstract »    Full Text »    PDF »
In Vitro Studies of the Prp9·Prp11·Prp21 Complex Indicate a Pathway for U2 Small Nuclear Ribonucleoprotein Activation.
D. K. Wiest, C. L. O'Day, and J. Abelson (1996)
J. Biol. Chem. 271, 33268-33276
   Abstract »    Full Text »    PDF »
CUS1, a suppressor of cold-sensitive U2 snRNA mutations, is a novel yeast splicing factor homologous to human SAP 145..
S E Wells, M Neville, M Haynes, J Wang, H Igel, and M Ares (1996)
Genes & Dev. 10, 220-232
   Abstract »    PDF »
Cloning and Functional Characterization of Human Selenophosphate Synthetase, an Essential Component of Selenoprotein Synthesis.
S. C. Low, J. W. Harney, and M. J. Berry (1995)
J. Biol. Chem. 270, 21659-21664
   Abstract »    Full Text »    PDF »
The prespliceosome components SAP 49 and SAP 145 interact in a complex implicated in tethering U2 snRNP to the branch site..
P Champion-Arnaud and R Reed (1994)
Genes & Dev. 8, 1974-1983
   Abstract »    PDF »
The yeast MUD2 protein: an interaction with PRP11 defines a bridge between commitment complexes and U2 snRNP addition..
N Abovich, X C Liao, and M Rosbash (1994)
Genes & Dev. 8, 843-854
   Abstract »    PDF »
Interaction of mammalian splicing factor SF3a with U2 snRNP and relation of its 60-kD subunit to yeast PRP9.
R Brosi, K Groning, S. Behrens, R Luhrmann, and A Kramer (1993)
Science 262, 102-105
   Abstract »    PDF »
Interaction between PRP11 and SPP91 yeast splicing factors and characterization of a PRP9-PRP11-SPP91 complex.
P Legrain and C Chapon (1993)
Science 262, 108-110
   Abstract »    PDF »


To Advertise     Find Products

Science. ISSN 0036-8075 (print), 1095-9203 (online)