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Science 1 October 1993:
Vol. 262. no. 5130, pp. 102 - 105
DOI: 10.1126/science.8211112
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Articles

Science, Vol 262, Issue 5130, 102-105
Copyright © 1993 by American Association for the Advancement of Science

articles

Interaction of mammalian splicing factor SF3a with U2 snRNP and relation of its 60-kD subunit to yeast PRP9

R Brosi, K Groning, SE Behrens, R Luhrmann, and A Kramer

Departement de Biologie Cellulaire, Sciences III, Universite de Geneve, Switzerland.

In the assembly of a prespliceosome, U2 small nuclear ribonucleoprotein (snRNP) functions in pre-messenger RNA (mRNA) splicing together with splicing factors (SFs) 3a, SF3b, and several other proteins. The 17S but not the 12S form of U2 snRNP is active in splicing-complex formation. Here it is shown that the SF3a subunits correspond to three of the 17S U2 snRNP-specific polypeptides. SF3a interacts with U2 snRNP in the presence of SF3b to generate a structure similar to 17S U2 snRNP, which suggests a function for SF3a and SF3b in the incorporation of U2 snRNP into the spliceosome. Furthermore, the 60-kilodalton subunit of SF3a is related to the yeast splicing protein PRP9.


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