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Science 10 September 1993:
Vol. 261. no. 5127, pp. 1454 - 1457
DOI: 10.1126/science.8367727
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Articles

Science, Vol 261, Issue 5127, 1454-1457
Copyright © 1993 by American Association for the Advancement of Science

articles

Cloning of an M. tuberculosis DNA fragment associated with entry and survival inside cells

S Arruda, G Bomfim, R Knights, T Huima-Byron, and LW Riley

Division of International Medicine, Cornell University Medical College, New York, NY 10021.

Mycobacterium tuberculosis infects one-third of the world's human population. This widespread infection depends on the organism's ability to escape host defenses by gaining entry and surviving inside the macrophage. DNA sequences of M. tuberculosis have been cloned; these confer on a nonpathogenic Escherichia coli strain an ability to invade HeLa cells, augment macrophage phagocytosis, and survive for at least 24 hours inside the human macrophage. This capacity to gain entry into mammalian cells and survive inside the macrophage was localized to two distinct loci on the cloned M. tuberculosis DNA fragment.


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