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Science 10 September 1993:
Vol. 261. no. 5127, pp. 1445 - 1448
DOI: 10.1126/science.7690156

Articles

Science, Vol 261, Issue 5127, 1445-1448
Copyright © 1993 by American Association for the Advancement of Science


articles

Inhibition of viral replication by interferon-gamma-induced nitric oxide synthase

G Karupiah, QW Xie, RM Buller, C Nathan, C Duarte, and JD MacMicking

Laboratory of Viral Diseases, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD 20892.

Interferons (IFNs) induce antiviral activity in many cell types. The ability of IFN-gamma to inhibit replication of ectromelia, vaccinia, and herpes simplex-1 viruses in mouse macrophages correlated with the cells' production of nitric oxide (NO). Viral replication was restored in IFN-gamma-treated macrophages exposed to inhibitors of NO synthase. Conversely, epithelial cells with no detectable NO synthesis restricted viral replication when transfected with a complementary DNA encoding inducible NO synthase or treated with organic compounds that generate NO. In mice, an inhibitor of NO synthase converted resolving ectromelia virus infection into fulminant mousepox. Thus, induction of NO synthase can be necessary and sufficient for a substantial antiviral effect of IFN-gamma.


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A Role for Transforming Growth Factor-beta 1 in the Increased Pneumonitis in Murine Allogeneic Bone Marrow Transplant Recipients With Graft-Versus-Host Disease After Pulmonary Herpes Simplex Virus Type 1 Infection.
H. Adler, J. L. Beland, W. Kozlow, N. C. Del-Pan, L. Kobzik, and I. J. Rimm (1998)
Blood 92, 2581-2589
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Resistance to Murine Hepatitis Virus Strain 3 Is Dependent on Production of Nitric Oxide.
M. Pope, P. A. Marsden, E. Cole, S. Sloan, L. S. Fung, Q. Ning, J. W. Ding, J. L. Leibowitz, M. J. Phillips, and G. A. Levy (1998)
J. Virol. 72, 7084-7090
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Identification of Nitric Oxide Synthase 2 as an Innate Resistance Locus against Ectromelia Virus Infection.
G. Karupiah, J.-H. Chen, C. F. Nathan, S. Mahalingam, and J. D. MacMicking (1998)
J. Virol. 72, 7703-7706
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Protective Role of Gamma Interferon during the Recall Response to Influenza Virus.
A. Bot, S. Bot, and C. A. Bona (1998)
J. Virol. 72, 6637-6645
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Double-stranded RNA-induced Inducible Nitric-oxide Synthase Expression and Interleukin-1 Release by Murine Macrophages Requires NF-kappa B Activation.
M. R. Heitmeier, A. L. Scarim, and J. A. Corbett (1998)
J. Biol. Chem. 273, 15301-15307
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Does Nitric Oxide Play a Critical Role in Viral Infections?.
C. S. Reiss and T. Komatsu (1998)
J. Virol. 72, 4547-4551
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The mosquito Anopheles stephensi limits malaria parasite development with inducible synthesis of nitric oxide.
S. Luckhart, Y. Vodovotz, L. Cui, and R. Rosenberg (1998)
PNAS 95, 5700-5705
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The role of inducible nitric oxide synthase in the host response to Coxsackievirus myocarditis.
C. Zaragoza, C. Ocampo, M. Saura, M. Leppo, X.-Q. Wei, R. Quick, S. Moncada, F. Y. Liew, and C. J. Lowenstein (1998)
PNAS 95, 2469-2474
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Protective Role of Nitric Oxide in Staphylococcus aureus Infection in Mice.
S. Sasaki, T. Miura, S. Nishikawa, K. Yamada, M. Hirasue, and A. Nakane (1998)
Infect. Immun. 66, 1017-1022
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Distinct Pathways for Tumor Necrosis Factor Alpha and Ceramides in Human Cytomegalovirus Infection.
J. Allan-Yorke, M. Record, C. de Preval, C. Davrinche, and J.-L. Davignon (1998)
J. Virol. 72, 2316-2322
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Effect of Nitric Oxide on Poliovirus Infection of Two Human Cell Lines.
J. A. Lopez-Guerrero and L. Carrasco (1998)
J. Virol. 72, 2538-2540
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Inhibition of NF-kappa B and HIV-1 Long Terminal Repeat Transcriptional Activation by Inducible Nitric Oxide Synthase 2 Activity.
D. Sekkai, F. Aillet, N. Israel, and M. Lepoivre (1998)
J. Biol. Chem. 273, 3895-3900
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Early Murine Cytomegalovirus (MCMV) Infection Induces Liver Natural Killer (NK) Cell Inflammation and Protection Through Macrophage Inflammatory Protein 1{alpha} (MIP-1{alpha})-dependent Pathways.
T. P. Salazar-Mather, J. S. Orange, and C. A. Biron (1998)
J. Exp. Med. 187, 1-14
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Interferon (IFN)-{alpha} Activation of Human Blood Mononuclear Cells In Vitro and In Vivo for Nitric Oxide Synthase (NOS) Type 2 mRNA and Protein Expression: Possible Relationship of Induced NOS2 to the Anti-Hepatitis C Effects of IFN-{alpha} In Vivo.
A. I. Sharara, D. J. Perkins, M. A. Misukonis, S. U. Chan, J. A. Dominitz, and J. B. Weinberg (1997)
J. Exp. Med. 186, 1495-1502
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Role of nitric oxide in poly(I-C)-induced endothelial cell expression of leukocyte adhesion molecules.
T. R. Faruqi, S. C. Erzurum, F. T. Kaneko, and P. E. Dicorleto (1997)
Am J Physiol Heart Circ Physiol 273, H2490-H2497
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Low-Dose N{omega}-Nitro-L-Arginine Methyl Ester Treatment Improves Survival Rate and Decreases Myocardial Injury in a Murine Model of Viral Myocarditis Induced by Coxsackievirus B3.
S. Mikami, S. Kawashima, K. Kanazawa, K.-i. Hirata, H. Hotta, Y. Hayashi, H. Itoh, and M. Yokoyama (1997)
Circ. Res. 81, 504-511
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