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Science 10 September 1993:
Vol. 261. no. 5127, pp. 1411 - 1418
DOI: 10.1126/science.7690155
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Articles

Science, Vol 261, Issue 5127, 1411-1418
Copyright © 1993 by American Association for the Advancement of Science

articles

Isolation of new ribozymes from a large pool of random sequences [see comment]

DP Bartel and JW Szostak

Department of Molecular Biology, Massachusetts General Hospital, Boston 02114.

An iterative in vitro selection procedure was used to isolate a new class of catalytic RNAs (ribozymes) from a large pool of random-sequence RNA molecules. These ribozymes ligate two RNA molecules that are aligned on a template by catalyzing the attack of a 3'-hydroxyl on an adjacent 5'-triphosphate--a reaction similar to that employed by the familiar protein enzymes that synthesize RNA. The corresponding uncatalyzed reaction also yields a 3',5'-phosphodiester bond. In vitro evolution of the population of new ribozymes led to improvement of the average ligation activity and the emergence of ribozymes with reaction rates 7 million times faster than the uncatalyzed reaction rate.


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