Note to users. If you're seeing this message, it means that your browser cannot find this page's style/presentation instructions -- or possibly that you are using a browser that does not support current Web standards. Find out more about why this message is appearing, and what you can do to make your experience of our site the best it can be.

Science 20 August 1993:
Vol. 261. no. 5124, pp. 1044 - 1047
DOI: 10.1126/science.8102497
Prev | Table of Contents | Next

Articles

Science, Vol 261, Issue 5124, 1044-1047
Copyright © 1993 by American Association for the Advancement of Science

articles

Localization of an exchangeable GTP binding site at the plus end of microtubules

TJ Mitchison

Department of Pharmacology, University of California, San Francisco 94143-0450.

Microtubule polarity arises from the head-to-tail orientation of alpha-beta tubulin heterodimers in the microtubule lattice. The identity of the polypeptide at each end of the microtubule is unknown, but structural models predict that the beta-tubulin end contains an exchangeable guanosine triphosphate (GTP) binding site. When GTP-coated fluorescent beads were incubated with microtubules, they bound specifically to plus ends, suggesting that tubulin is oriented in microtubules with beta-tubulin toward the plus end.


THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES:
Microtubule Plus-End Conformations and Dynamics in the Periphery of Interphase Mouse Fibroblasts.
S. Zovko, J. P. Abrahams, A. J. Koster, N. Galjart, and A. M. Mommaas (2008)
Mol. Biol. Cell 19, 3138-3146
   Abstract »    Full Text »    PDF »
Microtubule nucleation: {gamma}-tubulin and beyond.
C. Wiese and Y. Zheng (2006)
J. Cell Sci. 119, 4143-4153
   Abstract »    Full Text »    PDF »
The dynamic kinetochore-microtubule interface.
H. Maiato, J. DeLuca, E. D. Salmon, and W. C. Earnshaw (2004)
J. Cell Sci. 117, 5461-5477
   Abstract »    Full Text »    PDF »
Identification of XMAP215 as a microtubule-destabilizing factor in Xenopus egg extract by biochemical purification.
M. Shirasu-Hiza, P. Coughlin, and T. Mitchison (2003)
J. Cell Biol. 161, 349-358
   Abstract »    Full Text »    PDF »
Microtubule Nucleation from Stable Tubulin Oligomers.
N. Caudron, I. Arnal, E. Buhler, D. Job, and O. Valiron (2002)
J. Biol. Chem. 277, 50973-50979
   Abstract »    Full Text »    PDF »
A Mutation in gamma -Tubulin Alters Microtubule Dynamics and Organization and Is Synthetically Lethal with the Kinesin-like Protein Pkl1p.
J. L. Paluh, E. Nogales, B. R. Oakley, K. McDonald, A. L. Pidoux, and W. Z. Cande (2000)
Mol. Biol. Cell 11, 1225-1239
   Abstract »    Full Text »
The carboxy terminus of Tub4p is required for gamma-tubulin function in budding yeast.
J Vogel and M Snyder (2000)
J. Cell Sci. 113, 3871-3882
   Abstract »    PDF »
Direct Photoaffinity Labeling of Cysteine-295 of alpha -Tubulin by Guanosine 5'-Triphosphate Bound in the Nonexchangeable Site.
R. Bai, K. Choe, J. B. Ewell, N. Y. Nguyen, and E. Hamel (1998)
J. Biol. Chem. 273, 9894-9897
   Abstract »    Full Text »    PDF »
Expression of Amino- and Carboxyl-terminal gamma - and alpha -Tubulin Mutants in Cultured Epithelial Cells.
A. Leask and T. Stearns (1998)
J. Biol. Chem. 273, 2661-2668
   Abstract »    Full Text »    PDF »
A role for microtubule dynamics in phagosome movement.
A Blocker, G Griffiths, J. Olivo, A. Hyman, and F. Severin (1998)
J. Cell Sci. 111, 303-312
   Abstract »    PDF »
A Metastable Intermediate State of Microtubule Dynamic Instability That Differs Significantly between Plus and Minus Ends.
P.T. Tran, R.A. Walker, and E.D. Salmon (1997)
J. Cell Biol. 138, 105-117
   Abstract »    Full Text »    PDF »
Centrosomes Isolated from Spisula solidissima Oocytes Contain Rings and an Unusual Stoichiometric Ratio of {alpha}/{beta} Tubulin.
J. M. Vogel, T. Stearns, C. L. Rieder, and R. E. Palazzo (1997)
J. Cell Biol. 137, 193-202
   Abstract »    Full Text »    PDF »
Non-centrosomal microtubule formation and measurement of minus end microtubule dynamics in A498 cells.
A. Yvon and P Wadsworth (1997)
J. Cell Sci. 110, 2391-2401
   Abstract »    PDF »
Centrosome-microtubule nucleation.
G Pereira and E Schiebel (1997)
J. Cell Sci. 110, 295-300
   Abstract »    PDF »
Differential Effects of Vinblastine on Polymerization and Dynamics at Opposite Microtubule Ends.
D. Panda, M. A. Jordan, K. C. Chu, and L. Wilson (1996)
J. Biol. Chem. 271, 29807-29812
   Abstract »    Full Text »    PDF »
Role of gamma-tubulin in mitosis-specific microtubule nucleation from the Schizosaccharomyces pombe spindle pole body.
H Masuda and T Shibata (1996)
J. Cell Sci. 109, 165-177
   Abstract »    PDF »
Monomeric gamma -Tubulin Nucleates Microtubules.
R. Leguy, R. Melki, D. Pantaloni, and M.-F. Carlier (2000)
J. Biol. Chem. 275, 21975-21980
   Abstract »    Full Text »    PDF »


To Advertise     Find Products

Science. ISSN 0036-8075 (print), 1095-9203 (online)