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Science 6 August 1993:
Vol. 261. no. 5122, pp. 765 - 769
DOI: 10.1126/science.7688144
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Articles

Science, Vol 261, Issue 5122, 765-769
Copyright © 1993 by American Association for the Advancement of Science

articles

Primers for mitochondrial DNA replication generated by endonuclease G

J Cote and A Ruiz-Carrillo

Cancer Research Center, Medical School of Laval University, L'Hotel-Dieu de Quebec, Canada.

Endonuclease G (Endo G) is widely distributed among animals and cleaves DNA at double-stranded (dG)n.(dC)n and at single-stranded (dC)n tracts. Endo G is synthesized as a propeptide with an amino-terminal presequence that targets the nuclease to mitochondria. Endo G can also be detected in extranucleolar chromatin. In addition to deoxyribonuclease activities, Endo G also has ribonuclease (RNase) and RNase H activities and specifically cleaves mouse mitochondrial RNA and DNA-RNA substrates containing the origin of heavy-strand DNA replication (OH). The cleavage sites match those found in vivo, indicating that Endo G is capable of generating the RNA primers required by DNA polymerase gamma to initiate replication of mitochondrial DNA.


THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES:
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Expression of a novel non-coding mitochondrial RNA in human proliferating cells.
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Initiation of Mitochondrial DNA Replication by Transcription and R-loop Processing.
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J. Biol. Chem. 273, 30614-30621
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The POP1 gene encodes a protein component common to the RNase MRP and RNase P ribonucleoproteins..
Z Lygerou, P Mitchell, E Petfalski, B Seraphin, and D Tollervey (1994)
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