Note to users. If you're seeing this message, it means that your browser cannot find this page's style/presentation instructions -- or possibly that you are using a browser that does not support current Web standards. Find out more about why this message is appearing, and what you can do to make your experience of our site the best it can be.

Site Tools

  • AAAS
  • Subscribe
  • Feedback

Site Search

Search Advanced

Science 6 August 1993:
Vol. 261. no. 5122, pp. 759 - 762
DOI: 10.1126/science.8342041
Prev | Table of Contents | Next

Articles

Science, Vol 261, Issue 5122, 759-762
Copyright © 1993 by American Association for the Advancement of Science

articles

DNA melting on yeast RNA polymerase II promoters

C Giardina and JT Lis

Section of Biochemistry, Molecular, and Cell Biology, Cornell University, Ithaca, NY 14853.

Transcription-dependent DNA melting on the yeast GAL1 and GAL10 promoters was found to be more closely correlated with the TATA box than the transcription start site. On both these genes, melting begins about 20 base pairs downstream of the TATA box. Physical and genetic analyses suggest that RNA polymerase II associates with this region. Thus, the distance between promoter melting and the TATA box in yeast may be similar to that in higher eukaryotes, even though transcription initiates in a region about 10 to 90 base pairs farther downstream in yeast.


THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES:
Two strategies for gene regulation by promoter nucleosomes.
I. Tirosh and N. Barkai (2008)
Genome Res. 18, 1084-1091
   Abstract »    Full Text »    PDF »
Properties of an Intergenic Terminator and Start Site Switch That Regulate IMD2 Transcription in Yeast.
M. H. Jenks, T. W. O'Rourke, and D. Reines (2008)
Mol. Cell. Biol. 28, 3883-3893
   Abstract »    Full Text »    PDF »
Functions of Saccharomyces cerevisiae TFIIF during Transcription Start Site Utilization.
D. A. Khaperskyy, M. L. Ammerman, R. C. Majovski, and A. S. Ponticelli (2008)
Mol. Cell. Biol. 28, 3757-3766
   Abstract »    Full Text »    PDF »
Quantitative Analysis of in Vivo Initiator Selection by Yeast RNA Polymerase II Supports a Scanning Model.
J. N. Kuehner and D. A. Brow (2006)
J. Biol. Chem. 281, 14119-14128
   Abstract »    Full Text »    PDF »
Mapping of transcription start sites in Saccharomyces cerevisiae using 5' SAGE.
Z. Zhang and F. S. Dietrich (2005)
Nucleic Acids Res. 33, 2838-2851
   Abstract »    Full Text »    PDF »
Dissecting Transcription-coupled and Global Genomic Repair in the Chromatin of Yeast GAL1-10 Genes.
S. Li and M. J. Smerdon (2004)
J. Biol. Chem. 279, 14418-14426
   Abstract »    Full Text »    PDF »
Structural Basis of Transcription: An RNA Polymerase II-TFIIB Cocrystal at 4.5 Angstroms.
D. A. Bushnell, K. D. Westover, R. E. Davis, and R. D. Kornberg (2004)
Science 303, 983-988
   Abstract »    Full Text »    PDF »
Yeast RNA Polymerase II Lacking the Rpb9 Subunit Is Impaired for Interaction with Transcription Factor IIF.
L. M. Ziegler, D. A. Khaperskyy, M. L. Ammerman, and A. S. Ponticelli (2003)
J. Biol. Chem. 278, 48950-48956
   Abstract »    Full Text »    PDF »
Core Promoter-Dependent TFIIB Conformation and a Role for TFIIB Conformation in Transcription Start Site Selection.
J. A. Fairley, R. Evans, N. A. Hawkes, and S. G. E. Roberts (2002)
Mol. Cell. Biol. 22, 6697-6705
   Abstract »    Full Text »    PDF »
The role of TFIIB-RNA polymerase II interaction in start site selection in yeast cells.
D.-Y. Zhang, D. J. Carson, and J. Ma (2002)
Nucleic Acids Res. 30, 3078-3085
   Abstract »    Full Text »    PDF »
Promoter-Specific Shifts in Transcription Initiation Conferred by Yeast TFIIB Mutations Are Determined by the Sequence in the Immediate Vicinity of the Start Sites.
S. L. Faitar, S. A. Brodie, and A. S. Ponticelli (2001)
Mol. Cell. Biol. 21, 4427-4440
   Abstract »    Full Text »    PDF »
RNA Polymerase III Transcription Complexes on Chromosomal 5S rRNA Genes In Vivo: TFIIIB Occupancy and Promoter Opening.
G. Costanzo, S. Camier, P. Carlucci, L. Burderi, and R. Negri (2001)
Mol. Cell. Biol. 21, 3166-3178
   Abstract »    Full Text »
Promoter-Proximal Pausing on the hsp70 Promoter in Drosophila melanogaster Depends on the Upstream Regulator.
H. Tang, Y. Liu, L. Madabusi, and D. S. Gilmour (2000)
Mol. Cell. Biol. 20, 2569-2580
   Abstract »    Full Text »
Transcription Initiation at the TATA-less Spliced Leader RNA Gene Promoter Requires at Least Two DNA-binding Proteins and a Tripartite Architecture That Includes an Initiator Element.
H. Luo, G. Gilinger, D. Mukherjee, and V. Bellofatto (1999)
J. Biol. Chem. 274, 31947-31954
   Abstract »    Full Text »    PDF »
Mutational Analysis of Yeast TFIIB: A Functional Relationship Between Ssu72 and Sub1/Tsp1 Defined by Allele-Specific Interactions With TFIIB.
W.-H. Wu, I. Pinto, B.-S. Chen, and M. Hampsey (1999)
Genetics 153, 643-652
   Abstract »    Full Text »    PDF »
Evidence That Transcription Factor IIB Is Required for a Post-assembly Step in Transcription Initiation.
E.-J. Cho and S. Buratowski (1999)
J. Biol. Chem. 274, 25807-25813
   Abstract »    Full Text »    PDF »
Transcription Factor TFIIH Is Required for Promoter Melting In Vivo.
E. Guzman and J. T. Lis (1999)
Mol. Cell. Biol. 19, 5652-5658
   Abstract »    Full Text »    PDF »
Multiple Pathways of Recombination Induced by Double-Strand Breaks in Saccharomyces cerevisiae.
F. Paques and J. E. Haber (1999)
Microbiol. Mol. Biol. Rev. 63, 349-404
   Abstract »    Full Text »    PDF »
The Role of Human TFIIB in Transcription Start Site Selection in Vitro and in Vivo.
N. A. Hawkes and S. G. E. Roberts (1999)
J. Biol. Chem. 274, 14337-14343
   Abstract »    Full Text »    PDF »
The N-terminal Region of Yeast TFIIB Contains Two Adjacent Functional Domains Involved in Stable RNA Polymerase II Binding and Transcription Start Site Selection.
T. S. Pardee, C. S. Bangur, and A. S. Ponticelli (1998)
J. Biol. Chem. 273, 17859-17864
   Abstract »    Full Text »    PDF »
Molecular Genetics of the RNA Polymerase II General Transcriptional Machinery.
M. Hampsey (1998)
Microbiol. Mol. Biol. Rev. 62, 465-503
   Abstract »    Full Text »    PDF »
RNA Polymerase-DNA Interaction: Structures of Intermediate, Open, and Elongation Complexes.
R.H. EBRIGHT (1998)
Cold Spring Harb Symp Quant Biol 63, 11-20
   Abstract »    PDF »
Functional Analysis of TFIID Components.
W.-C. SHEN, L.M. APONE, C.-M.A. VIRBASIUS, X.-Y. LI, M. MONSALVE, and M.R. GREEN (1998)
Cold Spring Harb Symp Quant Biol 63, 219-228
   Abstract »    PDF »
Promoter-associated Pausing in Promoter Architecture and Postinitiation Transcriptional Regulation.
J. LIS (1998)
Cold Spring Harb Symp Quant Biol 63, 347-356
   Abstract »    PDF »
Nucleosome Transactions on the Promoters of the Yeast GAL and PHO Genes.
D. Lohr (1997)
J. Biol. Chem. 272, 26795-26798
   Full Text »    PDF »
Transcription Elongation through DNA Arrest Sites. A MULTISTEP PROCESS INVOLVING BOTH RNA POLYMERASE II SUBUNIT RPB9 AND TFIIS.
D. E. Awrey, R. G. Weilbaecher, S. A. Hemming, S. M. Orlicky, C. M. Kane, and A. M. Edwards (1997)
J. Biol. Chem. 272, 14747-14754
   Abstract »    Full Text »    PDF »
GAL4/GAL80-dependent Nucleosome Disruption/Deposition on the Upstream Regions of the Yeast GAL1-10 and GAL80 Genes.
D. Lohr and J. Lopez (1995)
J. Biol. Chem. 270, 27671-27678
   Abstract »    Full Text »    PDF »
Identification by in Organello Footprinting of Protein Contact Sites and of Single-stranded DNA Sequences in the Regulatory Region of Rat Mitochondrial DNA.
P. Cantatore, L. Daddabbo, F. Fracasso, and M. N. Gadaleta (1995)
J. Biol. Chem. 270, 25020-25027
   Abstract »    Full Text »    PDF »
Accelerated Hybridization of Oligonucleotides to Duplex DNA.
M. Iyer, J. C. Norton, and D. R. Corey (1995)
J. Biol. Chem. 270, 14712-14717
   Abstract »    Full Text »    PDF »
Sodium Salicylate and Yeast Heat Shock Gene Transcription.
C. Giardina and J. T. Lis (1995)
J. Biol. Chem. 270, 10369-10372
   Abstract »    Full Text »    PDF »
Functional Connections between Mediator Components and General Transcription Factors of Saccharomyces cerevisiae.
H. Sakurai and T. Fukasawa (2000)
J. Biol. Chem. 275, 37251-37256
   Abstract »    Full Text »    PDF »
An initiation element in the yeast CUP1 promoter is recognized by RNA polymerase II in the absence of TATA box-binding protein if the DNA is negatively supercoiled.
B. P. Leblanc, C. J. Benham, and D. J. Clark (2000)
PNAS 97, 10745-10750
   Abstract »    Full Text »    PDF »


To Advertise     Find Products

Science. ISSN 0036-8075 (print), 1095-9203 (online)