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Science 30 July 1993:
Vol. 261. no. 5121, pp. 617 - 619
DOI: 10.1126/science.8342028
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Articles

Science, Vol 261, Issue 5121, 617-619
Copyright © 1993 by American Association for the Advancement of Science

articles

The Caenorhabditis elegans unc-17 gene: a putative vesicular acetylcholine transporter

A Alfonso, K Grundahl, JS Duerr, HP Han, and JB Rand

Program in Molecular and Cell Biology, Oklahoma Medical Research Foundation, Oklahoma City 73104.

Mutations in the unc-17 gene of the nematode Caenorhabditis elegans produce deficits in neuromuscular function. This gene was cloned and complementary DNAs were sequenced. On the basis of sequence similarity to mammalian vesicular transporters of biogenic amines and of localization to synaptic vesicles of cholinergic neurons in C. elegans, unc-17 likely encodes the vesicular transporter of acetylcholine. Mutations that eliminated all unc-17 gene function were lethal, suggesting that the acetylcholine transporter is essential. Molecular analysis of unc-17 mutations will allow the correlation of specific parts of the gene (and the protein) with observed functional defects. The mutants will also be useful for the isolation of extragenic suppressors, which could identify genes encoding proteins that interact with UNC-17.


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