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Science 30 July 1993: Vol. 261. no. 5121, pp. 600 - 603 DOI: 10.1126/science.8342023
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Articles
Science, Vol 261, Issue 5121, 600-603
Copyright © 1993 by American Association for the Advancement of Science
Signal sequence trap: a cloning strategy for secreted proteins and type I membrane proteins
K Tashiro,
H Tada,
R Heilker,
M Shirozu,
T Nakano,
and
T Honjo
Department of Medical Chemistry, Kyoto University Faculty of Medicine, Japan.
A method was developed to clone, without the use of specific functional assays, complementary DNAs (cDNAs) that carry specific amino-terminal signal sequences, such as those encoding intercellular signal-transducing molecules and receptors. The vector used in this system directed the cell surface expression of interleukin-2 receptor fusion proteins when inserts with signal sequences were cloned in-frame with the correct orientation. An expression cDNA library was constructed from a bone marrow stromal cell line, which contained 5' portion-enriched cDNAs (the average size was 400 base pairs). Two cDNAs that encoded putative cytokine molecules, stromal cell-derived factor-1 alpha (SDF-1 alpha) and SDF-1 beta, which belong to the intercrine-macrophage inflammatory protein superfamily, were cloned.
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- AMD3100, a Potent and Specific Antagonist of the Stromal Cell-Derived Factor-1 Chemokine Receptor CXCR4, Inhibits Autoimmune Joint Inflammation in IFN-{gamma} Receptor-Deficient Mice.
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J. Immunol.
167, 4686-4692
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- Diverse Transcriptional Response of CD4+ T Cells to Stromal Cell-Derived Factor (SDF)-1: Cell Survival Promotion and Priming Effects of SDF-1 on CD4+ T Cells.
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- A Coordinated Change in Chemokine Responsiveness Guides Plasma Cell Movements.
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J. Exp. Med.
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- Increased Level of Stromal Cell-Derived Factor-1 mRNA in Peripheral Blood Mononuclear Cells from Children with AIDS-related Lymphoma.
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Cancer Res.
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- A novel regulator of G-protein signaling bearing GAP activity for G{alpha}i and G{alpha}q in megakaryocytes.
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Blood
97, 3051-3060
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- Replacement of the V3 Region of gp120 with SDF-1 Preserves the Infectivity of T-Cell Line-Tropic Human Immunodeficiency Virus Type 1.
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J. Virol.
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- Expression of Stromal-Derived Factor-1 Is Decreased by IL-1 and TNF and in Dermal Wound Healing.
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J. Immunol.
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- Stromal cell-derived factor 1{alpha} increases polyploidization of megakaryocytes generated by human hematopoietic progenitor cells.
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Blood
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- Coregulation of CXC Chemokine Receptor and CD4 Expression on T Lymphocytes During Allogeneic Activation.
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J. Immunol.
166, 4870-4878
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- Stromal Cell-Derived Factor-1-Induced LFA-1 Activation During In Vivo Migration of T Cell Hybridoma Cells Requires Gq/11, RhoA, and Myosin, as well as Gi and Cdc42.
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166, 4293-4301
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- Stromal derived factor-1{alpha} (SDF-1{alpha}) induces CD4+ T cell apoptosis via the functional up-regulation of the Fas (CD95)/Fas ligand (CD95L) pathway.
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- Chemokine stromal cell-derived factor-1{alpha} modulates VLA-4 integrin-mediated multiple myeloma cell adhesion to CS-1/fibronectin and VCAM-1.
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Blood
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- Lack of correlation between chemokine receptor and Th1/Th2 cytokine expression by individual memory T cells.
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Int. Immunol.
12, 1659-1667
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- Stromal Cell-Derived Factor-1-CXC Chemokine Receptor 4 Interactions Play a Central Role in CD4+ T Cell Accumulation in Rheumatoid Arthritis Synovium.
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- Impairment of lymphopoiesis and myelopoiesis in mice reconstituted with bone marrow-hematopoietic progenitor cells expressing SDF-1-intrakine.
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Blood
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- Increased Expression of H/T-Cadherin in Tumor-penetrating Blood Vessels.
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- Netrin-G1: a Novel Glycosyl Phosphatidylinositol-Linked Mammalian Netrin That Is Functionally Divergent from Classical Netrins.
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- Expression of Stromal Cell-derived Factor 1 and CXCR4 Ligand Receptor System in Pancreatic Cancer: A Possible Role for Tumor Progression.
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- Gi and Gq/11 proteins are involved in dissemination of myeloid leukemia cells to the liver and spleen, whereas bone marrow colonization involves Gq/11 but not Gi.
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Blood
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- Cloning of murine glycosyl phosphatidylinositol anchor attachment protein, GPAA1.
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