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Science 23 July 1993:
Vol. 261. no. 5120, pp. 486 - 489
DOI: 10.1126/science.8392754
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Articles

Science, Vol 261, Issue 5120, 486-489
Copyright © 1993 by American Association for the Advancement of Science

articles

Subunit identification and reconstitution of the N-type Ca2+ channel complex purified from brain

DR Witcher, M De Waard, J Sakamoto, C Franzini-Armstrong, M Pragnell, SD Kahl, and KP Campbell

Howard Hughes Medical Institute, Department of Physiology and Biophysics, University of Iowa College of Medicine, Iowa City 52242.

Calcium channels play an important role in regulating various neuronal processes, including synaptic transmission and cellular plasticity. The N-type calcium channels, which are sensitive to omega-conotoxin, are involved in the control of transmitter release from neurons. A functional N-type calcium channel complex was purified from rabbit brain. The channel consists of a 230-kilodalton subunit (alpha 1B) that is tightly associated with a 160-kilodalton subunit (alpha 2 delta), a 57-kilodalton subunit (beta 3), and a 95-kilodalton glycoprotein subunit. The complex formed a functional calcium channel with the same pharmacological properties and conductance as those of the native omega-conotoxin-sensitive calcium channel in neurons.


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