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Science 9 July 1993:
Vol. 261. no. 5118, pp. 218 - 221
DOI: 10.1126/science.8327893
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Articles

Science, Vol 261, Issue 5118, 218-221
Copyright © 1993 by American Association for the Advancement of Science

articles

Inhibition of adenylyl cyclase by Gi alpha

R Taussig, JA Iniguez-Lluhi, and AG Gilman

Department of Pharmacology, University of Texas Southwestern Medical Center, Dallas 75235-9041.

Evidence suggests that both alpha and beta gamma subunits of heterotrimeric guanine nucleotide-binding regulatory proteins (G proteins) inhibit adenylyl cyclase. Although type I adenylyl cyclase is inhibited directly by exogenous beta gamma, inhibition of adenylyl cyclase by Gi alpha has not been convincingly demonstrated in vitro. Concentration-dependent inhibition of adenylyl cyclases by purified Gi alpha subunits is described. Activated Gi alpha but not G(o) alpha was effective, and myristoylation of Gi alpha was required. The characteristics of the inhibitory effect were dependent on the type of adenylyl cyclase and the nature of the activator of the enzyme. The concentrations of Gi alpha required to inhibit adenylyl cyclase were substantially higher than those normally thought to be relevant physiologically. However, analysis indicates that these concentrations may be relevant and reasonable.


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K. S. Murthy and G. M. Makhlouf (1997)
J. Biol. Chem. 272, 21317-21324
   Abstract »    Full Text »    PDF »
Identification of Common and Distinct Residues Involved in the Interaction of alpha i2 and alpha s with Adenylyl Cyclase.
G. Grishina and C. H. Berlot (1997)
J. Biol. Chem. 272, 20619-20626
   Abstract »    Full Text »    PDF »
Gq-Protein Alpha Subunit Expression and Distribution in Pregnant Rat Myometrial Tissues.
C. M. Harris, T. Saunders, D. A. Baunoch, and M. Phillippe (1997)
Reproductive Sciences 4, 209-217
   Abstract »    PDF »
Gsalpha contains an unidentified covalent modification that increases its affinity for adenylyl cyclase.
C. Kleuss and A. G. Gilman (1997)
PNAS 94, 6116-6120
   Abstract »    Full Text »    PDF »
Insulin-induced Activation of NADPH-dependent H2O2 Generation in Human Adipocyte Plasma Membranes Is Mediated by Galpha i2.
H. I. Krieger-Brauer, P. K. Medda, and H. Kather (1997)
J. Biol. Chem. 272, 10135-10143
   Abstract »    Full Text »    PDF »
The Adenylyl Cyclases as Integrators of Transmembrane Signal Transduction.
Y. Ishikawa and C. J. Homcy (1997)
Circ. Res. 80, 297-304
   Full Text »
Opiate-induced Adenylyl Cyclase Superactivation Is Isozyme-specific.
T. Avidor-Reiss, I. Nevo, D. Saya, M. Bayewitch, and Z. Vogel (1997)
J. Biol. Chem. 272, 5040-5047
   Abstract »    Full Text »    PDF »
Differential Regulation of Type I and Type VIII Ca2+-stimulated Adenylyl Cyclases by Gi-coupled Receptors in Vivo.
M. D. Nielsen, G. C.K. Chan, S. W. Poser, and D. R. Storm (1996)
J. Biol. Chem. 271, 33308-33316
   Abstract »    Full Text »    PDF »
Protein Kinase C Alters the Responsiveness of Adenylyl Cyclases to G Protein alpha and beta gamma Subunits.
G. Zimmermann and R. Taussig (1996)
J. Biol. Chem. 271, 27161-27166
   Abstract »    Full Text »    PDF »
Localization of the Effector-specifying Regions of Gi2alpha and Gqalpha.
R. Medina, G. Grishina, E. G. Meloni, T. R. Muth, and C. H. Berlot (1996)
J. Biol. Chem. 271, 24720-24727
   Abstract »    Full Text »    PDF »
Chronic Opioid Treatment Induces Adenylyl Cyclase V Superactivation. INVOLVEMENT OF Gbeta gamma.
T. Avidor-Reiss, I. Nevo, R. Levy, T. Pfeuffer, and Z. Vogel (1996)
J. Biol. Chem. 271, 21309-21315
   Abstract »    Full Text »    PDF »
Regulation of Membrane and Subunit Interactions by N-Myristoylation of a G Protein alpha Subunit in Yeast.
J. Song, J. Hirschman, K. Gunn, and H. G. Dohlman (1996)
J. Biol. Chem. 271, 20273-20283
   Abstract »    Full Text »    PDF »
The AT2 Receptor Selectively Associates with Gialpha 2 and Gialpha 3 in the Rat Fetus.
J. Zhang and R. E. Pratt (1996)
J. Biol. Chem. 271, 15026-15033
   Abstract »    Full Text »    PDF »
Identification and Characterization of a Widely Expressed Form of Adenylyl Cyclase.
R. T. Premont, I. Matsuoka, M.-G. Mattei, Y. Pouille, N. Defer, and J. Hanoune (1996)
J. Biol. Chem. 271, 13900-13907
   Abstract »    Full Text »    PDF »
Functional Importance of the Amino Terminus of G[IMAGE].
J. R. Hepler, G. H. Biddlecome, C. Kleuss, L. A. Camp, S. L. Hofmann, E. M. Ross, and A. G. Gilman (1996)
J. Biol. Chem. 271, 496-504
   Abstract »    Full Text »    PDF »
Galpha(i) RNA Antisense Expression Demonstrates the Exclusive Coupling of Peptide YY Receptors to G[IMAGE] Proteins in Renal Proximal Tubule Cells.
T. Voisin, A.-M. Lorinet, J.-J. Maoret, A. Couvineau, and M. Laburthe (1996)
J. Biol. Chem. 271, 574-580
   Abstract »    Full Text »    PDF »
Tertiary and Quaternary Structural Changes in Gialpha Induced by GTP Hydrolysis.
M. B. Mixon, E. Lee, D. E. Coleman, A. M. Berghuis, A. G. Gilman, and S. R. Sprang (1995)
Science 270, 954-960
   Abstract »    PDF »
Functional Expression of an Epitope-tagged G protein-coupled K[IMAGE] Channel (GIRK1).
L. H. Philipson, A. Kuznetsov, P. T. Toth, J. F. Murphy, G. Szabo, G. H. Ma, and R. J. Miller (1995)
J. Biol. Chem. 270, 14604-14610
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Fatty Acylation of [IMAGE][IMAGE].
P. T. Wilson and H. R. Bourne (1995)
J. Biol. Chem. 270, 9667-9675
   Abstract »    Full Text »    PDF »
Identification of Functional Domains of Adenylyl Cyclase Using in Vivo Chimeras.
L. R. Levin and R. R. Reed (1995)
J. Biol. Chem. 270, 7573-7579
   Abstract »    Full Text »    PDF »
Functional Analysis of a Dominant Negative Mutant of Galpha[IMAGE].
V. Z. Slepak, A. Katz, and M. I. Simon (1995)
J. Biol. Chem. 270, 4037-4041
   Abstract »    Full Text »    PDF »
Lipid Modifications of Trimeric G Proteins.
P. B. Wedegaertner, P. T. Wilson, and H. R. Bourne (1995)
J. Biol. Chem. 270, 503-506
   Full Text »    PDF »
Mammalian Membrane-bound Adenylyl Cyclases.
R. Taussig and A. G. Gilman (1995)
J. Biol. Chem. 270, 1-4
   Full Text »    PDF »


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Science. ISSN 0036-8075 (print), 1095-9203 (online)