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Science 2 July 1993:
Vol. 261. no. 5117, pp. 82 - 86
DOI: 10.1126/science.8316859
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Articles

Science, Vol 261, Issue 5117, 82-86
Copyright © 1993 by American Association for the Advancement of Science

articles

Regulation of lymphoid-specific immunoglobulin mu heavy chain gene enhancer by ETS-domain proteins

B Nelsen, G Tian, B Erman, J Gregoire, R Maki, B Graves, and R Sen

Rosenstiel Research Center, Brandeis University, Waltham, MA 02254.

The enhancer for the immunoglobulin mu heavy chain gene (IgH) activates a heterologous gene at the pre-B cell stage of B lymphocyte differentiation. A lymphoid-specific element, microB, is necessary for enhancer function in pre-B cells. A microB binding protein is encoded by the PU.1/Spi-1 proto-oncogene. Another sequence element, microA, was identified in the mu enhancer that binds the product of the ets-1 proto-oncogene. The microA motif was required for microB-dependent enhancer activity, which suggests that a minimal B cell-specific enhancer is composed of both the PU.1 and Ets-1 binding sites. Co-expression of both PU.1 and Ets-1 in nonlymphoid cells trans-activated reporter plasmids that contained the minimal mu enhancer. These results implicate two members of the Ets family in the activation of IgH gene expression.


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A Three-protein-DNA Complex on a B Cell-specific Domain of the ImmunoglobulinµHeavy Chain Gene Enhancer.
E. Rao, W. Dang, G. Tian, and R. Sen (1997)
J. Biol. Chem. 272, 6722-6732
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Overexpression of PU.1 Induces Growth and Differentiation Inhibition and Apoptotic Cell Death in Murine Erythroleukemia Cells.
T. Yamada, N. Kondoh, M. Matsumoto, M. Yoshida, A. Maekawa, and T. Oikawa (1997)
Blood 89, 1383-1393
   Abstract »    Full Text »    PDF »
ELF-1 Interacts with and Transactivates the IgH Enhancer pi Site.
Y. Akbarali, P. Oettgen, J. Boltax, and T. A. Libermann (1996)
J. Biol. Chem. 271, 26007-26012
   Abstract »    Full Text »    PDF »
Octamer Binding Factors and Their Coactivator Can Activate the Murine PU.1 (spi-1) Promoter.
H.-m. Chen, P. Zhang, H. S. Radomska, C. J. Hetherington, D.-E. Zhang, and D. G. Tenen (1996)
J. Biol. Chem. 271, 15743-15752
   Abstract »    Full Text »    PDF »
The immunoglobulin heavy-chain matrix-associating regions are bound by Bright: a B cell-specific trans-activator that describes a new DNA-binding protein family..
R F Herrscher, M H Kaplan, D L Lelsz, C Das, R Scheuermann, and P W Tucker (1995)
Genes & Dev. 9, 3067-3082
   Abstract »    PDF »
Repression of I-Abeta Gene Expression by the Transcription Factor PU.1.
F. E. Borràs, J. LLoberas, R. A. Maki, and A. Celada (1995)
J. Biol. Chem. 270, 24385-24391
   Abstract »    Full Text »    PDF »
GABP and PU.1 Compete for Binding, yet Cooperate to Increase CD18 (beta(2) Leukocyte Integrin) Transcription.
A. G. Rosmarin, D. G. Caprio, D. G. Kirsch, H. Handa, and C. P. Simkevich (1995)
J. Biol. Chem. 270, 23627-23633
   Abstract »    Full Text »    PDF »
ERM, a PEA3 Subfamily of Ets Transcription Factors, Can Cooperate with c-Jun.
K. Nakae, K. Nakajima, J. Inazawa, T. Kitaoka, and T. Hirano (1995)
J. Biol. Chem. 270, 23795-23800
   Abstract »    Full Text »    PDF »
Involvement of the Ets Family Factor PU.1 in the Activation of Immunoglobulin Promoters.
H. Schwarzenbach, J. W. Newell, and P. Matthias (1995)
J. Biol. Chem. 270, 898-907
   Abstract »    Full Text »    PDF »
Requirement of transcription factor PU.1 in the development of multiple hematopoietic lineages.
E. Scott, M. Simon, J Anastasi, and H Singh (1994)
Science 265, 1573-1577
   Abstract »    PDF »
The immunoglobulin mu enhancer core establishes local factor access in nuclear chromatin independent of transcriptional stimulation..
T Jenuwein, W C Forrester, R G Qiu, and R Grosschedl (1993)
Genes & Dev. 7, 2016-2032
   Abstract »    PDF »
PU.1-mediated Transcription Is Enhanced by HMG-I(Y)-dependent Structural Mechanisms.
R. T. Lewis, A. Andreucci, and B. S. Nikolajczyk (2001)
J. Biol. Chem. 276, 9550-9557
   Abstract »    Full Text »    PDF »


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Science. ISSN 0036-8075 (print), 1095-9203 (online)