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Science 18 June 1993:
Vol. 260. no. 5115, pp. 1796 - 1799
DOI: 10.1126/science.8511587
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Articles

Science, Vol 260, Issue 5115, 1796-1799
Copyright © 1993 by American Association for the Advancement of Science

articles

Interspecific and intraspecific horizontal transfer of Wolbachia in Drosophila

L Boyle, SL O'Neill, HM Robertson, and TL Karr

Department of Biochemistry, University of Illinois, Urbana 61801.

Cytoplasmic incompatibility (CI) in Drosophila simulans is related to infection of the germ line by a rickettsial endosymbiont (genus Wolbachia). Wolbachia were transferred by microinjection of egg cytoplasm into uninfected eggs of both D. simulans and D. melanogaster to generate infected populations. Transinfected strains of D. melanogaster with lower densities of Wolbachia than the naturally infected D. simulans strain did not express high levels of CI. However, transinfected D. melanogaster egg cytoplasm, transferred back into D. simulans, generated infected populations that expressed CI at levels near those of the naturally infected strain. A transinfected D. melanogaster line selected for increased levels of CI expression also displayed increased symbiont densities. These data suggest that a threshold level of infection is required for normal expression of CI and that host factors help determine the density of the symbiont in the host.


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