Note to users. If you're seeing this message, it means that your browser cannot find this page's style/presentation instructions -- or possibly that you are using a browser that does not support current Web standards. Find out more about why this message is appearing, and what you can do to make your experience of our site the best it can be.

Science 23 April 1993:
Vol. 260. no. 5107, pp. 504 - 508
DOI: 10.1126/science.7682726
Prev | Table of Contents | Next

Articles

Science, Vol 260, Issue 5107, 504-508
Copyright © 1993 by American Association for the Advancement of Science

articles

Movement of the guide sequence during RNA catalysis by a group I ribozyme

JF Wang, WD Downs, and TR Cech

Howard Hughes Medical Institute, Department of Chemistry and Biochemistry, University of Colorado, Boulder 80309.

Ribozymes derived from the self-splicing pre-ribosomal RNA of Tetrahymena act as sequence-specific endonucleases. The reaction involves binding an RNA or DNA substrate by base pairing to the internal guide sequence (IGS) to form helix P1. Site-specific photo-crosslinking localized the 5' end of the IGS in helix P1 to the vicinity of conserved bases between helices P4 and P5, supporting a major feature of the Michel-Westhof three-dimensional structure model. The crosslinked ribozyme retained catalytic activity. When not base-paired, the IGS was still specifically crosslinked, but the major site was 37 A distant from the reactive site in the experimentally supported three-dimensional model. The data indicate that a substantial induced-fit conformational change accompanies P1 formation, and they provide a physical basis for understanding the transport of oligonucleotides to the catalytic core of the ribozyme. The ability of RNA to orchestrate large-scale conformational changes may help explain why the ribosome and the spliceosome are RNA-based machines.


THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES:
A modular, bifunctional RNA that integrates itself into a target RNA.
R. M. Kumar and G. F. Joyce (2003)
PNAS 100, 9738-9743
   Abstract »    Full Text »    PDF »
A Single-Molecule Study of RNA Catalysis and Folding.
X. Zhuang, L. E. Bartley, H. P. Babcock, R. Russell, T. Ha, D. Herschlag, and S. Chu (2000)
Science 288, 2048-2051
   Abstract »    Full Text »
An important base triple anchors the substrate helix recognition surface within the Tetrahymena ribozyme active site.
A. A. Szewczak, L. Ortoleva-Donnelly, M. V. Zivarts, A. K. Oyelere, A. V. Kazantsev, and S. A. Strobel (1999)
PNAS 96, 11183-11188
   Abstract »    Full Text »    PDF »
A Preorganized Active Site in the Crystal Structure of the Tetrahymena Ribozyme.
B. L. Golden, A. R. Gooding, E. R. Podell, and T. R. Cech (1998)
Science 282, 259-264
   Abstract »    Full Text »
Defining the chemical groups essential for Tetrahymena group I intron function by nucleotide analog interference mapping.
S. A. Strobel and K. Shetty (1997)
PNAS 94, 2903-2908
   Abstract »    Full Text »    PDF »
Protein-induced Folding of a Group I Intron in Cytochrome b Pre-mRNA.
L. C. Shaw and A. S. Lewin (1995)
J. Biol. Chem. 270, 21552-21562
   Abstract »    Full Text »    PDF »
Minor groove recognition of the conserved G.U pair at the Tetrahymena ribozyme reaction site.
S. Strobel and T. Cech (1995)
Science 267, 675-679
   Abstract »    PDF »
Coaxially stacked RNA helices in the catalytic center of the Tetrahymena ribozyme.
F. Murphy, Y. Wang, J. Griffith, and T. Cech (1994)
Science 265, 1709-1712
   Abstract »    PDF »
Kinetic intermediates in RNA folding.
P. Zarrinkar and Williamson JR (1994)
Science 265, 918-924
   Abstract »    PDF »
A group III intron is formed from domains of two individual group II introns..
L Hong and R B Hallick (1994)
Genes & Dev. 8, 1589-1599
   Abstract »    PDF »
The U5 and U6 small nuclear RNAs as active site components of the spliceosome.
E. Sontheimer and J. Steitz (1993)
Science 262, 1989-1996
   Abstract »    PDF »


To Advertise     Find Products

Science. ISSN 0036-8075 (print), 1095-9203 (online)