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Science 16 April 1993:
Vol. 260. no. 5106, pp. 361 - 364
DOI: 10.1126/science.8469989
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Articles

Science, Vol 260, Issue 5106, 361-364
Copyright © 1993 by American Association for the Advancement of Science

articles

Tumor cell growth arrest caused by subchromosomal transferable DNA fragments from chromosome 11

M Koi, LA Johnson, LM Kalikin, PF Little, Y Nakamura, and AP Feinberg

Howard Hughes Medical Institute, Department of Internal Medicine, University of Michigan Medical School, Ann Arbor 48109.

A fundamental problem in the identification and isolation of tumor suppressor and other growth-inhibiting genes is the loss of power of genetic complementation at the subchromosomal level. A direct genetic strategy was developed to isolate subchromosomal transferable fragments (STFs) from any chromosome, each containing a selectable marker within the human DNA, that could be transferred to any mammalian cell. As a test of the method, several overlapping STFs from 11p15 were shown to cause in vitro growth arrest of rhabdomyosarcoma cells. This activity mapped between the beta-globin and insulin genes.


THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES:
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Adenovirus-Mediated Ribonucleotide Reductase R1 Gene Therapy of Human Colon Adenocarcinoma.
M.-Y. Cao, Y. Lee, N.-P. Feng, K. Xiong, H. Jin, M. Wang, A. Vassilakos, S. Viau, J. A. Wright, and A. H. Young (2003)
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Is the locus for Costello syndrome on 11p?.
B Kerr, M L Mucchielli, S Sigaudy, M Fabre, P Saunier, M A Voelckel, E Howard, R Elles, T O B Eden, G C Black, et al. (2003)
J. Med. Genet. 40, 469-471
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SIRT3, a human SIR2 homologue, is an NAD- dependent deacetylase localized to mitochondria.
P. Onyango, I. Celic, J. M. McCaffery, J. D. Boeke, and A. P. Feinberg (2002)
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Loss of Imprinting of Insulin-Like Growth Factor-II (IGF2) Gene in Distinguishing Specific Biologic Subtypes of Wilms Tumor.
J. D. Ravenel, K. W. Broman, E. J. Perlman, E. L. Niemitz, T. M. Jayawardena, D. W. Bell, D. A. Haber, H. Uejima, and A. P. Feinberg (2001)
J Natl Cancer Inst 93, 1698-1703
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Evidence for a Putative Senescence Gene Locus within the Chromosomal Region 10p14-p15.
M. Poignee, C. Backsch, K. Beer, L. Jansen, N. Wagenbach, E. J. Stanbridge, R. Kirchmayr, A. Schneider, and M. Durst (2001)
Cancer Res. 61, 7118-7121
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Sequence and Comparative Analysis of the Mouse 1-Megabase Region Orthologous to the Human 11p15 Imprinted Domain.
P. Onyango, W. Miller, J. Lehoczky, C. T. Leung, B. Birren, S. Wheelan, K. Dewar, and A. P. Feinberg (2000)
Genome Res. 10, 1697-1710
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The Chromosome 10 Monosomy Common in Human Melanomas Results from the Loss of Two Separate Tumor Suppressor Loci.
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H19 Overexpression in Breast Adenocarcinoma Stromal Cells Is Associated with Tumor Values and Steroid Receptor Status but Independent of p53 and Ki-67 Expression.
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Aberrant Splicing of the TSG101 Tumor Suppressor Gene in Human Breast and Ovarian Cancers.
M. E. Carney, G. L. Maxwell, J. M. Lancaster, C. Gumbs, J. Marks, A. Berchuck, and P. A. Futreal (1998)
Reproductive Sciences 5, 281-285
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In vitro loss of heterozygosity targets the PTEN/MMAC1 gene in melanoma.
G. P. Robertson, F. B. Furnari, M. E. Miele, M. J. Glendening, D. R. Welch, J. W. Fountain, T. G. Lugo, H.-J. S. Huang, and W. K. Cavenee (1998)
PNAS 95, 9418-9423
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Loss of Heterozygosity on Chromosome 11p15 during Histological Progression in Microdissected Ductal Carcinoma of the Breast.
J. H. Lichy, M. Zavar, M. M. Tsai, T. J. O'Leary, and J. K. Taubenberger (1998)
Am. J. Pathol. 153, 271-278
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Rescue of Targeted Regions of Mammalian Chromosomes by in Vivo Recombination in Yeast.
N. Kouprina, K. Kawamoto, J. C. Barrett, V. Larionov, and M. Koi (1998)
Genome Res. 8, 666-672
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Transcriptional map of 170-kb region at chromosome 11p15.5: Identification and mutational analysis of the BWR1A gene reveals the presence of mutations in tumor samples.
C. Schwienbacher, S. Sabbioni, M. Campi, A. Veronese, G. Bernardi, A. Menegatti, I. Hatada, T. Mukai, H. Ohashi, G. Barbanti-Brodano, et al. (1998)
PNAS 95, 3873-3878
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Specific chromosomal imbalances in human papillomavirus-transfected cells during progression toward immortality.
S. Solinas-Toldo, M. Durst, and P. Lichter (1997)
PNAS 94, 3854-3859
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Human repeat-mediated integration of selectable markers into somatic cell hybrids..
J E Watson, E M Slorach, J Maule, D Lawson, D J Porteous, and A J Brookes (1995)
Genome Res. 5, 444-452
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Inhibitors of mammalian G1 cyclin-dependent kinases..
C J Sherr and J M Roberts (1995)
Genes & Dev. 9, 1149-1163
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Loss of Imprinting in Human Cancer.
A.P. Feinberg, L.M. Kalikin, L.A. Johnson, and J.S. Thompson (1994)
Cold Spring Harb Symp Quant Biol 59, 357-364
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Cellular Senescence and Cancer.
J.C. Barrett, L.A. Annab, D. Alcorta, G. Preston, P. Vojta, and Y. Yin (1994)
Cold Spring Harb Symp Quant Biol 59, 411-418
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WT1-mediated growth suppression of Wilms tumor cells expressing a WT1 splicing variant.
D. Haber, S Park, S Maheswaran, C Englert, G. Re, D. Hazen-Martin, D. Sens, and A. Garvin (1993)
Science 262, 2057-2059
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