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Science 16 April 1993:
Vol. 260. no. 5106, pp. 361 - 364
DOI: 10.1126/science.8469989
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Articles

Science, Vol 260, Issue 5106, 361-364
Copyright © 1993 by American Association for the Advancement of Science

articles

Tumor cell growth arrest caused by subchromosomal transferable DNA fragments from chromosome 11

M Koi, LA Johnson, LM Kalikin, PF Little, Y Nakamura, and AP Feinberg

Howard Hughes Medical Institute, Department of Internal Medicine, University of Michigan Medical School, Ann Arbor 48109.

A fundamental problem in the identification and isolation of tumor suppressor and other growth-inhibiting genes is the loss of power of genetic complementation at the subchromosomal level. A direct genetic strategy was developed to isolate subchromosomal transferable fragments (STFs) from any chromosome, each containing a selectable marker within the human DNA, that could be transferred to any mammalian cell. As a test of the method, several overlapping STFs from 11p15 were shown to cause in vitro growth arrest of rhabdomyosarcoma cells. This activity mapped between the beta-globin and insulin genes.


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Is the locus for Costello syndrome on 11p?.
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Evidence for a Putative Senescence Gene Locus within the Chromosomal Region 10p14-p15.
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Specific chromosomal imbalances in human papillomavirus-transfected cells during progression toward immortality.
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Human repeat-mediated integration of selectable markers into somatic cell hybrids..
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Inhibitors of mammalian G1 cyclin-dependent kinases..
C J Sherr and J M Roberts (1995)
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WT1-mediated growth suppression of Wilms tumor cells expressing a WT1 splicing variant.
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