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Science 16 April 1993:
Vol. 260. no. 5106, pp. 349 - 352
DOI: 10.1126/science.8469986
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Articles

Science, Vol 260, Issue 5106, 349-352
Copyright © 1993 by American Association for the Advancement of Science

articles

Transient transfection and expression in the obligate intracellular parasite Toxoplasma gondii

D Soldati and JC Boothroyd

Department of Microbiology and Immunology, Stanford University School of Medicine, CA 94305.

Toxoplasma gondii is a protozoan pathogen that produces severe disease in humans and animals. This obligate intracellular parasite provides an excellent model for the study of how such pathogens are able to invade, survive, and replicate intracellularly. DNA encoding chloramphenicol acetyltransferase was introduced into T. gondii and transiently expressed with the use of three vectors based on different Toxoplasma genes. The ability to introduce genes and have them efficiently and faithfully expressed is an essential tool for understanding the structure-function relation of genes and their products.


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