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Science 9 April 1993:
Vol. 260. no. 5105, pp. 238 - 241
DOI: 10.1126/science.8097062

Articles

Science, Vol 260, Issue 5105, 238-241
Copyright © 1993 by American Association for the Advancement of Science


articles

Regulation of CREB phosphorylation in the suprachiasmatic nucleus by light and a circadian clock

DD Ginty, JM Kornhauser, MA Thompson, H Bading, KE Mayo, JS Takahashi, and ME Greenberg

Department of Microbiology and Molecular Genetics, Harvard Medical School, Boston, MA 02115.

Mammalian circadian rhythms are regulated by a pacemaker within the suprachiasmatic nuclei (SCN) of the hypothalamus. The molecular mechanisms controlling the synchronization of the circadian pacemaker are unknown; however, immediate early gene (IEG) expression in the SCN is tightly correlated with entrainment of SCN-regulated rhythms. Antibodies were isolated that recognize the activated, phosphorylated form of the transcription factor cyclic adenosine monophosphate response element binding protein (CREB). Within minutes after exposure of hamsters to light, CREB in the SCN became phosphorylated on the transcriptional regulatory site, Ser133. CREB phosphorylation was dependent on circadian time: CREB became phosphorylated only at times during the circadian cycle when light induced IEG expression and caused phase shifts of circadian rhythms. These results implicate CREB in neuronal signaling in the hypothalamus and suggest that circadian clock gating of light-regulated molecular responses in the SCN occurs upstream of phosphorylation of CREB.


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Ca2+-Permeable AMPA Receptors Induce Phosphorylation of cAMP Response Element-Binding Protein through a Phosphatidylinositol 3-Kinase-Dependent Stimulation of the Mitogen-Activated Protein Kinase Signaling Cascade in Neurons.
M. S. Perkinton, T. S. Sihra, and R. J. Williams (1999)
J. Neurosci. 19, 5861-5874
   Abstract »    Full Text »    PDF »
Mechanisms of Lung Neutrophil Activation After Hemorrhage or Endotoxemia: Roles of Reactive Oxygen Intermediates, NF-{kappa}B, and Cyclic AMP Response Element Binding Protein.
R. Shenkar and E. Abraham (1999)
J. Immunol. 163, 954-962
   Abstract »    Full Text »    PDF »
Transcriptional and posttranslational plasticity and the generation of inflammatory pain.
C. J. Woolf and M. Costigan (1999)
PNAS 96, 7723-7730
   Abstract »    Full Text »    PDF »
Transcriptional Regulation of the Interleukin-6 Gene in Mesangial Cells.
C. GRASSL, B. LUCKOW, D. SCHLÖNDORFF, and U. DENDORFER (1999)
J. Am. Soc. Nephrol. 10, 1466-1477
   Abstract »    Full Text »
Circadian Regulation of cAMP Response Element-mediated Gene Expression in the Suprachiasmatic Nuclei.
K. Obrietan, S. Impey, D. Smith, J. Athos, and D. R. Storm (1999)
J. Biol. Chem. 274, 17748-17756
   Abstract »    Full Text »    PDF »
Abnormalities in Neuronal Process Extension, Hippocampal Development, and the Ventricular System of L1 Knockout Mice.
G. P. Demyanenko, A. Y. Tsai, and P. F. Maness (1999)
J. Neurosci. 19, 4907-4920
   Abstract »    Full Text »    PDF »
Transcription Factors in Neuroendocrine Regulation: Rhythmic Changes in pCREB and ICER Levels Frame Melatonin Synthesis.
E. Maronde, M. Pfeffer, J. Olcese, C. A. Molina, F. Schlotter, F. Dehghani, H.-W. Korf, and J. H. Stehle (1999)
J. Neurosci. 19, 3326-3336
   Abstract »    Full Text »    PDF »
Molecular Correlates of Topographic Reorganization in Primary Visual Cortex Following Retinal Lesions.
S. Obata, J. Obata, A. Das, and C. D. Gilbert (1999)
Cereb Cortex 9, 238-248
   Abstract »    Full Text »    PDF »
Prostaglandin E-Prostanoid-3 Receptor Activation of Cyclic AMP Response Element-Mediated Gene Transcription.
L. P. Audoly, L. Ma, I. Feoktistov, S. K. de Foe, M. D. Breyer, and R. M. Breyer (1999)
J. Pharmacol. Exp. Ther. 289, 140-148
   Abstract »    Full Text »
The Type and the Localization of cAMP-dependent Protein Kinase Regulate Transmission of cAMP Signals to the Nucleus in Cortical and Cerebellar Granule Cells.
M. Paolillo, A. Feliciello, A. Porcellini, C. Garbi, M. Bifulco, S. Schinelli, C. Ventra, E. Stabile, G. Ricciardelli, G. Schettini, et al. (1999)
J. Biol. Chem. 274, 6546-6552
   Abstract »    Full Text »    PDF »
Novel Effects on the Gonyaulax Circadian System Produced by the Protein Kinase Inhibitor Staurosporine.
J. C. Comolli and J. W. Hastings (1999)
J Biol Rhythms 14, 11-19
   Abstract »    PDF »
Rapid Resetting of the Mammalian Circadian Clock.
J. D. Best, E. S. Maywood, K. L. Smith, and M. H. Hastings (1999)
J. Neurosci. 19, 828-835
   Abstract »    Full Text »    PDF »
Differing temporal roles of Ca2+ and cAMP in nicotine-elicited elevation of tyrosine hydroxylase mRNA.
V. D. Gueorguiev, R. J. Zeman, B. Hiremagalur, A. Menezes, and E. L. Sabban (1999)
Am J Physiol Cell Physiol 276, C54-C65
   Abstract »    Full Text »    PDF »
Ceramide and Cyclic Adenosine Monophosphate (cAMP) Induce cAMP Response Element Binding Protein Phosphorylation via Distinct Signaling Pathways While Having Opposite Effects on Myeloid Cell Survival.
M. P. Scheid, I. N. Foltz, P. R. Young, J. W. Schrader, and V. Duronio (1999)
Blood 93, 217-225
   Abstract »    Full Text »    PDF »
Repetitive transcranial magnetic stimulation activates specific regions in rat brain.
R.-R. Ji, T. E. Schlaepfer, C. D. Aizenman, C. M. Epstein, D. Qiu, J. C. Huang, and F. Rupp (1998)
PNAS 95, 15635-15640
   Abstract »    Full Text »    PDF »
CREB in the Mouse SCN: A Molecular Interface Coding the Phase-Adjusting Stimuli Light, Glutamate, PACAP, and Melatonin for Clockwork Access.
C. von Gall, G. E. Duffield, M. H. Hastings, M. D. A. Kopp, F. Dehghani, H.-W. Korf, and J. H. Stehle (1998)
J. Neurosci. 18, 10389-10397
   Abstract »    Full Text »    PDF »



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