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Science 2 April 1993:
Vol. 260. no. 5104, pp. 82 - 84
DOI: 10.1126/science.8385366
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Articles

Science, Vol 260, Issue 5104, 82-84
Copyright © 1993 by American Association for the Advancement of Science

articles

Rhythmic exocytosis stimulated by GnRH-induced calcium oscillations in rat gonadotropes

A Tse, FW Tse, W Almers, and B Hille

Department of Physiology and Biophysics, University of Washington School of Medicine, Seattle 98195.

In pituitary gonadotropes, gonadotropin-releasing hormone (GnRH) induces the rhythmic release of Ca2+ from an inositol 1,4,5-trisphosphate (IP3)-sensitive store. Simultaneous measurement of the concentration of cytosolic free Ca2+ ([Ca2+]i) and exocytosis in single identified gonadotropes showed that each elevation of [Ca2+]i induced a burst of exocytosis. These phenomena were largely suppressed by buffering of [Ca2+]i but persisted in the absence of extracellular Ca2+. Activation of voltage-gated Ca2+ channels by brief depolarizations seldom supplied enough Ca2+ for exocytosis, but [Ca2+]i elevations induced by photolysis of caged IP3 did trigger exocytosis, confirming that GnRH-stimulated gonadotropic hormone secretion is closely coupled to intracellular Ca2+ release. Agonist-induced oscillations of [Ca2+]i in secretory cells may be a mechanism to optimize the secretory output while avoiding the toxic effects of sustained elevation of [Ca2+]i.


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