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Science 26 March 1993:
Vol. 259. no. 5103, pp. 1899 - 1901
DOI: 10.1126/science.8456315
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Articles

Science, Vol 259, Issue 5103, 1899-1901
Copyright © 1993 by American Association for the Advancement of Science

articles

Antibody-catalyzed degradation of cocaine

DW Landry, K Zhao, GX Yang, M Glickman, and TM Georgiadis

Department of Medicine, Columbia University College of Physicians and Surgeons, New York, NY 10032.

Immunization with a phosphonate monoester transition-state analog of cocaine provided monoclonal antibodies capable of catalyzing the hydrolysis of the cocaine benzoyl ester group. An assay for the degradation of radiolabeled cocaine identified active enzymes. Benzoyl esterolysis yields ecgonine methyl ester and benzoic acid, fragments devoid of cocaine's stimulant activity. Passive immunization with such an artificial enzyme could provide a treatment for dependence by blunting reinforcement.


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