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Science 26 March 1993:
Vol. 259. no. 5103, pp. 1892 - 1896
DOI: 10.1126/science.8456313
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Articles

Science, Vol 259, Issue 5103, 1892-1896
Copyright © 1993 by American Association for the Advancement of Science

articles

Structural relationship of bacterial RecA proteins to recombination proteins from bacteriophage T4 and yeast

RM Story, DK Bishop, N Kleckner, and TA Steitz

Department of Molecular Biophysics, Yale University, New Haven, CT 06511.

RecA protein is essential in eubacteria for homologous recombination and promotes the homologous pairing and strand exchange of DNA molecules in vitro. Recombination proteins with weak sequence similarity to bacterial RecA proteins have been identified in bacteriophage T4, yeast, and other higher organisms. Analysis of the primary sequence relationships of DMC1 from Saccharomyces cerevisiae and UvsX of T4 relative to the three-dimensional structure of RecA from Escherichia coli suggests that both proteins are structural homologs of bacterial RecA proteins. This analysis argues that proteins in this group are members of a single family that diverged from a common ancestor that existed prior to the divergence of prokaryotes and eukaryotes.


THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES:
Red-Hed regulation: recombinase Rad51, though capable of playing the leading role, may be relegated to supporting Dmc1 in budding yeast meiosis..
S. Sheridan and D. K. Bishop (2006)
Genes & Dev. 20, 1685-1691
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Distinct roles for two RAD51-related genes in Trypanosoma brucei antigenic variation.
C. Proudfoot and R. McCulloch (2005)
Nucleic Acids Res. 33, 6906-6919
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Five RecA-like Proteins of Schizosaccharomyces pombe Are Involved in Meiotic Recombination.
A. L. Grishchuk and J. Kohli (2003)
Genetics 165, 1031-1043
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Rad51 Recombinase and Recombination Mediators.
P. Sung, L. Krejci, S. Van Komen, and M. G. Sehorn (2003)
J. Biol. Chem. 278, 42729-42732
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C-terminal Deletions of the Escherichia coli RecA Protein. CHARACTERIZATION OF IN VIVO AND IN VITRO EFFECTS.
S. L. Lusetti, E. A. Wood, C. D. Fleming, M. J. Modica, J. Korth, L. Abbott, D. W. Dwyer, A. I. Roca, R. B. Inman, and M. M. Cox (2003)
J. Biol. Chem. 278, 16372-16380
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Identification and purification of two distinct complexes containing the five RAD51 paralogs.
J.-Y. Masson, M. C. Tarsounas, A. Z. Stasiak, A. Stasiak, R. Shah, M. J. McIlwraith, F. E. Benson, and S. C. West (2001)
Genes & Dev. 15, 3296-3307
   Abstract »    Full Text »    PDF »
DNA Pairing and Strand Exchange by the Escherichia coli RecA and Yeast Rad51 Proteins without ATP Hydrolysis. ON THE IMPORTANCE OF NOT GETTING STUCK.
K. P. Rice, A. L. Eggler, P. Sung, and M. M. Cox (2001)
J. Biol. Chem. 276, 38570-38581
   Abstract »    Full Text »    PDF »
Historical overview: Searching for replication help in all of the rec places.
M. M. Cox (2001)
PNAS 98, 8173-8180
   Abstract »    Full Text »    PDF »
Gain- and loss-of-function of Rhp51, a Rad51 homolog in fission yeast, reveals dissimilarities in chromosome integrity.
W. J. Kim, H. Lee, E. J. Park, J. K. Park, and S. D. Park (2001)
Nucleic Acids Res. 29, 1724-1732
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Intracellular ATP Increases Capsaicin-Activated Channel Activity by Interacting with Nucleotide-Binding Domains.
J. Kwak, M. H. Wang, S. W. Hwang, T.-Y. Kim, S.-Y. Lee, and U. Oh (2000)
J. Neurosci. 20, 8298-8304
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The role of BicD, egl, orb and the microtubules in the restriction of meiosis to the Drosophila oocyte.
J Huynh and D St Johnston (2000)
Development 127, 2785-2794
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The Bacterial Replicative Helicase DnaB Evolved from a RecA Duplication.
D. D. Leipe, L. Aravind, N. V. Grishin, and E. V. Koonin (2000)
Genome Res. 10, 5-16
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A role for RAD51 and homologous recombination in Trypanosoma brucei antigenic variation.
R. McCulloch and J. D. Barry (1999)
Genes & Dev. 13, 2875-2888
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RAD51 and DMC1 Form Mixed Complexes Associated with Mouse Meiotic Chromosome Cores and Synaptonemal Complexes.
M. Tarsounas, T. Morita, R. E. Pearlman, and P. B. Moens (1999)
J. Cell Biol. 147, 207-220
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The Essential Functions of Human Rad51 Are Independent of ATP Hydrolysis.
C. Morrison, A. Shinohara, E. Sonoda, Y. Yamaguchi-Iwai, M. Takata, R. R. Weichselbaum, and S. Takeda (1999)
Mol. Cell. Biol. 19, 6891-6897
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Human Dmc1 protein binds DNA as an octameric ring.
S. I. Passy, X. Yu, Z. Li, C. M. Radding, J.-Y. Masson, S. C. West, and E. H. Egelman (1999)
PNAS 96, 10684-10688
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Diversity of radA Genes from Cultured and Uncultured Archaea: Comparative Analysis of Putative RadA Proteins and Their Use as a Phylogenetic Marker.
S. J. Sandler, P. Hugenholtz, C. Schleper, E. F. DeLong, N. R. Pace, and A. J. Clark (1999)
J. Bacteriol. 181, 907-915
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A Role for RAD51 in the Generation of Immunoglobulin Gene Diversity in Rabbits.
R. A. Barrington, M. Fasullo, and K. L. Knight (1999)
J. Immunol. 162, 911-919
   Abstract »    Full Text »    PDF »
okra and spindle-B encode components of the RAD52 DNA repair pathway and affect meiosis and patterning in Drosophila oogenesis.
A. Ghabrial, R. P. Ray, and T. Schüpbach (1998)
Genes & Dev. 12, 2711-2723
   Abstract »    Full Text »
RadA protein is an archaeal RecA protein homolog that catalyzes DNA strand exchange.
E. M. Seitz, J. P. Brockman, S. J. Sandler, A. J. Clark, and S. C. Kowalczykowski (1998)
Genes & Dev. 12, 1248-1253
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RecA-like proteins are components of early meiotic nodules in lily.
L. K. Anderson, H. H. Offenberg, W. M. H. C. Verkuijlen, and C. Heyting (1997)
PNAS 94, 6868-6873
   Abstract »    Full Text »    PDF »
Strandedness Discrimination in Peptide-Polynucleotide Complexes.
N. P. Johnson, H. Mazarguil, and A. Lopez (1996)
J. Biol. Chem. 271, 19675-19679
   Abstract »    Full Text »    PDF »
The DNA Binding Site(s) of the Escherichia coli RecA Protein.
W. M. Rehrauer and S. C. Kowalczykowski (1996)
J. Biol. Chem. 271, 11996-12002
   Abstract »    Full Text »    PDF »
Roles for two RecA homologs in promoting meiotic chromosome synapsis..
B Rockmill, M Sym, H Scherthan, and G S Roeder (1995)
Genes & Dev. 9, 2684-2695
   Abstract »    PDF »
Homotypic and heterotypic protein associations control Rad51 function in double-strand break repair..
J W Donovan, G T Milne, and D T Weaver (1994)
Genes & Dev. 8, 2552-2562
   Abstract »    PDF »
Catalysis of ATP-dependent homologous DNA pairing and strand exchange by yeast RAD51 protein.
P Sung (1994)
Science 265, 1241-1243
   Abstract »    PDF »
The Sep1 strand exchange protein from Saccharomyces cerevisiae promotes a paranemic joint between homologous DNA molecules..
J Chen, R Kanaar, and N R Cozzarelli (1994)
Genes & Dev. 8, 1356-1366
   Abstract »    PDF »


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