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Science 19 March 1993:
Vol. 259. no. 5102, pp. 1760 - 1763
DOI: 10.1126/science.7681220

Articles

Science, Vol 259, Issue 5102, 1760-1763
Copyright © 1993 by American Association for the Advancement of Science


articles

An osmosensing signal transduction pathway in yeast

JL Brewster, T de Valoir, ND Dwyer, E Winter, and MC Gustin

Department of Biochemistry and Cell Biology, Rice University, Houston, TX 77251.

Yeast genes were isolated that are required for restoring the osmotic gradient across the cell membrane in response to increased external osmolarity. Two of these genes, HOG1 and PBS2, encode members of the mitogen-activated protein kinase (MAP kinase) and MAP kinase kinase gene families, respectively. MAP kinases are activated by extracellular ligands such as growth factors and function as intermediate kinases in protein phosphorylation cascades. A rapid, PBS2-dependent tyrosine phosphorylation of HOG1 protein occurred in response to increases in extracellular osmolarity. These data define a signal transduction pathway that is activated by changes in the osmolarity of the extracellular environment.


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S. Hohmann (2002)
Microbiol. Mol. Biol. Rev. 66, 300-372
   Abstract »    Full Text »    PDF »
The Serine/Threonine Kinase Cmk2 Is Required for Oxidative Stress Response in Fission Yeast.
M. Sanchez-Piris, F. Posas, V. Alemany, I. Winge, E. Hidalgo, O. Bachs, and R. Aligue (2002)
J. Biol. Chem. 277, 17722-17727
   Abstract »    Full Text »    PDF »
Transient Inhibition of Translation Initiation by Osmotic Stress.
Y. Uesono and A. Toh-e (2002)
J. Biol. Chem. 277, 13848-13855
   Abstract »    Full Text »    PDF »
Altered Phosphotransfer in an Activated Mutant of the Saccharomyces cerevisiae Two-Component Osmosensor Sln1p.
A. D. Ault, J. S. Fassler, and R. J. Deschenes (2002)
Eukaryot. Cell 1, 174-180
   Abstract »    Full Text »    PDF »
The Drosophila inebriated-Encoded Neurotransmitter/Osmolyte Transporter: Dual Roles in the Control of Neuronal Excitability and the Osmotic Stress Response.
X. Huang, Y. Huang, R. Chinnappan, C. Bocchini, M. C. Gustin, and M. Stern (2002)
Genetics 160, 561-569
   Abstract »    Full Text »    PDF »
Osmotic Shock Induces G1 Arrest through p53 Phosphorylation at Ser33 by Activated p38MAPK without Phosphorylation at Ser15 and Ser20.
H. Kishi, K. Nakagawa, M. Matsumoto, M. Suga, M. Ando, Y. Taya, and M. Yamaizumi (2001)
J. Biol. Chem. 276, 39115-39122
   Abstract »    Full Text »    PDF »
Perturbation of the Nucleus: A Novel Hog1p-independent, Pkc1p-dependent Consequence of Hypertonic Shock in Yeast.
J. Nanduri and A. M. Tartakoff (2001)
Mol. Biol. Cell 12, 1835-1841
   Abstract »    Full Text »    PDF »
Rck2, a member of the calmodulin-protein kinase family, links protein synthesis to high osmolarity MAP kinase signaling in budding yeast.
M. Teige, E. Scheikl, V. Reiser, H. Ruis, and G. Ammerer (2001)
PNAS 98, 5625-5630
   Abstract »    Full Text »    PDF »
Hyperosmotic Stress Induces the Rapid Phosphorylation of a Soybean Phosphatidylinositol Transfer Protein Homolog through Activation of the Protein Kinases SPK1 and SPK2.
D. E. Monks, K. Aghoram, P. D. Courtney, D. B. DeWald, and R. E. Dewey (2001)
PLANT CELL 13, 1205-1219
   Abstract »    Full Text »
Activation of Phosphatidylinositol 3-Kinase and Akt by tert-Butylhydroquinone Is Responsible for Antioxidant Response Element-Mediated rGSTA2 Induction in H4IIE Cells.
K. W. Kang, M. K. Cho, C. H. Lee, and S. G. Kim (2001)
Mol. Pharmacol. 59, 1147-1156
   Abstract »    Full Text »
Mitogen-Activated Protein (MAP) Kinase Pathways: Regulation and Physiological Functions.
G. Pearson, F. Robinson, T. Beers Gibson, B.-e Xu, M. Karandikar, K. Berman, and M. H. Cobb (2001)
Endocr. Rev. 22, 153-183
   Abstract »    Full Text »
Hypertonicity-induced phosphorylation and nuclear localization of the transcription factor TonEBP.
S. C. Dahl, J. S. Handler, and H. M. Kwon (2001)
Am J Physiol Cell Physiol 280, C248-C253
   Abstract »    Full Text »    PDF »
Peroxide Sensors for the Fission Yeast Stress-activated Mitogen-activated Protein Kinase Pathway.
V. Buck, J. Quinn, T. S. Pino, H. Martin, J. Saldanha, K. Makino, B. A. Morgan, and J. B.A. Millar (2001)
Mol. Biol. Cell 12, 407-419
   Abstract »    Full Text »



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