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Science 19 March 1993:
Vol. 259. no. 5102, pp. 1749 - 1754
DOI: 10.1126/science.8096089
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Articles

Science, Vol 259, Issue 5102, 1749-1754
Copyright © 1993 by American Association for the Advancement of Science

articles

High levels of HIV-1 in plasma during all stages of infection determined by competitive PCR

M Piatak Jr, MS Saag, LC Yang, SJ Clark, JC Kappes, KC Luk, BH Hahn, GM Shaw, and JD Lifson

Division of HIV and Exploratory Research, Genelabs Technologies Inc., Redwood City, CA 94063.

Quantitative competitive polymerase chain reaction (QC-PCR) methods were used to quantify virion-associated human immunodeficiency virus type-1 (HIV-1) RNA in plasma from 66 patients with Centers for Disease Control stage I to IVC1 infection. HIV-1 RNA, ranging from 100 to nearly 22,000,000 copies per milliliter of plasma (corresponding to 50 to 11,000,000 virions per milliliter), was readily quantified in all subjects, was significantly associated with disease stage and CD4+ T cell counts, and decreased by as much as 235-fold with resolution of primary infection or institution of antiretroviral therapy. Plasma virus levels determined by QC-PCR correlated with, but exceeded by an average of 60,000-fold, virus titers measured by endpoint dilution culture. Quantitation of HIV-1 in plasma by QC-PCR may be useful in assessing the efficacy of antiretroviral agents, especially in early stage disease when conventional viral markers are often negative.


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Scientific and social issues of human immunodeficiency virus vaccine development.
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