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Science 12 March 1993:
Vol. 259. no. 5101, pp. 1614 - 1616
DOI: 10.1126/science.8384375
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Articles

Science, Vol 259, Issue 5101, 1614-1616
Copyright © 1993 by American Association for the Advancement of Science

articles

Expression cloning and signaling properties of the rat glucagon receptor

LJ Jelinek, S Lok, GB Rosenberg, RA Smith, FJ Grant, S Biggs, PA Bensch, JL Kuijper, PO Sheppard, CA Sprecher, and al. et

ZymoGenetics Inc., Seattle, WA 98105.

Glucagon and the glucagon receptor are a primary source of control over blood glucose concentrations and are especially important to studies of diabetes in which the loss of control over blood glucose concentrations clinically defines the disease. A complementary DNA clone for the glucagon receptor was isolated by an expression cloning strategy, and the receptor protein was expressed in several kidney cell lines. The cloned receptor bound glucagon and caused an increase in the intracellular concentration of adenosine 3', 5'-monophosphate (cAMP). The cloned glucagon receptor also transduced a signal that led to an increased concentration of intracellular calcium. The glucagon receptor is similar to the calcitonin and parathyroid hormone receptors. It can transduce signals leading to the accumulation of two different second messengers, cAMP and calcium.


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Two Cytoplasmic Loops of the Glucagon Receptor Are Required to Elevate cAMP or Intracellular Calcium.
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Regulation of Glucagon-Like Peptide-1 Synthesis and Secretion in the GLUTag Enteroendocrine Cell Line.
P. L. Brubaker, J. Schloos, and D. J. Drucker (1998)
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The New Biology of Gastrointestinal Hormones.
J. F. REHFELD (1998)
Physiol Rev 78, 1087-1108
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Parathyroid Hormone-Receptor Interactions Identified Directly by Photocross-linking and Molecular Modeling Studies.
A. Bisello, A. E. Adams, D. F. Mierke, M. Pellegrini, M. Rosenblatt, L. J. Suva, and M. Chorev (1998)
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Glucagon-mediated Ca2+ signaling in BHK cells expressing cloned human glucagon receptors.
L. H. Hansen, J. Gromada, P. Bouchelouche, T. Whitmore, L. Jelinek, W. Kindsvogel, and E. Nishimura (1998)
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R. R. Ryan, H. C. Weber, W. Hou, E. Sainz, S. A. Mantey, J. F. Battey, D. H. Coy, and R. T. Jensen (1998)
J. Biol. Chem. 273, 13613-13624
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Identification of Binding Domains of the Growth Hormone-Releasing Hormone Receptor by Analysis of Mutant and Chimeric Receptor Proteins.
V. I. DeAlmeida and K. E. Mayo (1998)
Mol. Endocrinol. 12, 750-765
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Positively Charged Residues at Positions 12, 17, and 18 of Glucagon Ensure Maximum Biological Potency.
C. G. Unson, C.-R. Wu, C. P. Cheung, and R. B. Merrifield (1998)
J. Biol. Chem. 273, 10308-10312
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In Vivo and in Vitro Regulation of Hepatic Glucagon Receptor mRNA Concentration by Glucose Metabolism.
R. Burcelin, C. Mrejen, J. F. Decaux, S. H. De Mouzon, J. Girard, and M. J. Charron (1998)
J. Biol. Chem. 273, 8088-8093
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alpha -Latrotoxin Receptor, Latrophilin, Is a Novel Member of the Secretin Family of G Protein-coupled Receptors.
V. G. Lelianova, B. A. Davletov, A. Sterling, M. A. Rahman, E. V. Grishin, N. F. Totty, and Y. A. Ushkaryov (1997)
J. Biol. Chem. 272, 21504-21508
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Alterations in Receptor Activation and Divalent Cation Activation of Agonist Binding by Deletion of Intracellular Domains of the Glucagon Receptor.
G. G. Chicchi, M. P. Graziano, G. Koch, P. Hey, K. Sullivan, P. P. Vicario, and M. A. Cascieri (1997)
J. Biol. Chem. 272, 7765-7769
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Mutations in the Second Cytoplasmic Loop of the Rat Parathyroid Hormone (PTH)/PTH-related Protein Receptor Result in Selective Loss of PTH-stimulated Phospholipase C Activity.
A. Iida-Klein, J. Guo, M. Takemura, M. T. Drake, J. T. Potts Jr., A. Abou-Samra, F. R. Bringhurst, and G. V. Segre (1997)
J. Biol. Chem. 272, 6882-6889
   Abstract »    Full Text »    PDF »
Molecular Cloning of a Novel Variant of the Pituitary Adenylate Cyclase-activating Polypeptide (PACAP) Receptor That Stimulates Calcium Influx by Activation of L-type Calcium Channels.
T. K. Chatterjee, R. V. Sharma, and R. A. Fisher (1996)
J. Biol. Chem. 271, 32226-32232
   Abstract »    Full Text »    PDF »
Molecular Cloning and Characterization of the Human Anaphylatoxin C3a Receptor.
R. S. Ames, Y. Li, H. M. Sarau, P. Nuthulaganti, J. J. Foley, C. Ellis, Z. Zeng, K. Su, A. J. Jurewicz, R. P. Hertzberg, et al. (1996)
J. Biol. Chem. 271, 20231-20234
   Abstract »    Full Text »    PDF »
Converting Parathyroid Hormone-related Peptide (PTHrP) into a Potent PTH-2 Receptor Agonist.
T. J. Gardella, M. D. Luck, G. S. Jensen, T. B. Usdin, and H. Juppner (1996)
J. Biol. Chem. 271, 19888-19893
   Abstract »    Full Text »    PDF »
Cloning and Characterization of the Signal Transduction of Four Splice Variants of the Human Pituitary Adenylate Cyclase Activating Polypeptide Receptor. EVIDENCE FOR DUAL COUPLING TO ADENYLATE CYCLASE AND PHOSPHOLIPASE C.
J. R. Pisegna and S. A. Wank (1996)
J. Biol. Chem. 271, 17267-17274
   Abstract »    Full Text »    PDF »
The Genomic Structure of the Rat Corticotropin Releasing Factor Receptor. A MEMBER OF THE CLASS II G PROTEIN-COUPLED RECEPTORS.
C. H. Tsai-Morris, E. Buczko, Y. Geng, A. Gamboa-Pinto, and M. L. Dufau (1996)
J. Biol. Chem. 271, 14519-14525
   Abstract »    Full Text »    PDF »
Transmembrane Residues of the Parathyroid Hormone (PTH)/PTH-related Peptide Receptor That Specifically Affect Binding and Signaling by Agonist Ligands.
T. J. Gardella, M. D. Luck, M.-H. Fan, and C. Lee (1996)
J. Biol. Chem. 271, 12820-12825
   Abstract »    Full Text »    PDF »
A cDNA Encoding the Calcitonin Gene-related Peptide Type 1 Receptor.
N. Aiyar, K. Rand, N. A. Elshourbagy, Z. Zeng, J. E. Adamou, D. J. Bergsma, and Y. Li (1996)
J. Biol. Chem. 271, 11325-11329
   Abstract »    Full Text »    PDF »
Protein Kinase A-dependent Phosphorylation of GLUT2 in Pancreatic beta Cells.
B. Thorens, N. Dériaz, D. Bosco, A. DeVos, D. Pipeleers, F. Schuit, P. Meda, and A.ée Porret (1996)
J. Biol. Chem. 271, 8075-8081
   Abstract »    Full Text »    PDF »
Characterization of Deletion and Truncation Mutants of the Rat Glucagon Receptor.
C. G. Unson, A. M. Cypess, H. N. Kim, P. K. Goldsmith, C. J. L. Carruthers, R. B. Merrifield, and T. P. Sakmar (1995)
J. Biol. Chem. 270, 27720-27727
   Abstract »    Full Text »    PDF »
Interaction of [fluorescein-Trp[IMAGE]]Glucagon with the Human Glucagon Receptor Expressed in Drosophila Schneider 2 Cells.
M. R. Tota, L. Xu, A. Sirotina, C. D. Strader, and M. P. Graziano (1995)
J. Biol. Chem. 270, 26466-26472
   Abstract »    Full Text »    PDF »
Hormone Stimulation of Type III Adenylyl Cyclase Induces Ca[IMAGE] Oscillations in HEK-293 Cells.
G. A. Wayman, T. R. Hinds, and D. R. Storm (1995)
J. Biol. Chem. 270, 24108-24115
   Abstract »    Full Text »    PDF »
Ca[IMAGE] Inhibition of Type III Adenylyl Cyclase in Vivo.
G. A. Wayman, S. Impey, and D. R. Storm (1995)
J. Biol. Chem. 270, 21480-21486
   Abstract »    Full Text »    PDF »
Proteolysis of Glucagon within Hepatic Endosomes by Membrane-associated Cathepsins B and D.
F. Authier, J. S. Mort, A. W. Bell, B. I. Posner, and J. J. M. Bergeron (1995)
J. Biol. Chem. 270, 15798-15807
   Abstract »    Full Text »    PDF »
Regulation of Glucagon Receptor mRNA in Cultured Primary Rat Hepatocytes by Glucose and cAMP.
N. Abrahamsen, K. Lundgren, and E. Nishimura (1995)
J. Biol. Chem. 270, 15853-15857
   Abstract »    Full Text »    PDF »
Truncation of the Carboxyl-terminal Region of the Rat Parathyroid Hormone (PTH)/PTH-related Peptide Receptor Enhances PTH Stimulation of Adenylyl Cyclase but Not Phospholipase C.
A. Iida-Klein, J. Guo, L. Y. Xie, H. Jüppner, J. T. Potts Jr., H. M. Kronenberg, F. R. Bringhurst, A. B. Abou-Samra, and G. V. Segre (1995)
J. Biol. Chem. 270, 8458-8465
   Abstract »    Full Text »    PDF »
GlucagonbulletGlucagon-like Peptide I Receptor Chimeras Reveal Domains That Determine Specificity of Glucagon Binding.
J. J. Buggy, J. N. Livingston, D. U. Rabin, and H. Yoo-Warren (1995)
J. Biol. Chem. 270, 7474-7478
   Abstract »    Full Text »    PDF »
Selective Stabilization of the High Affinity Binding Conformation of Glucagon Receptor by the Long Splice Variant of Galpha s.
C. G. Unson, C.-R. Wu, T. P. Sakmar, and R. B. Merrifield (2000)
J. Biol. Chem. 275, 21631-21638
   Abstract »    Full Text »    PDF »
The Glucagon-like Peptide-2 Receptor Mediates Direct Inhibition of Cellular Apoptosis via a cAMP-dependent Protein Kinase-independent Pathway.
B. Yusta, R. P. Boushey, and D. J. Drucker (2000)
J. Biol. Chem. 275, 35345-35352
   Abstract »    Full Text »    PDF »
Glucagon receptor activates extracellular signal-regulated protein kinase 1/2 via cAMP-dependent protein kinase.
Y. Jiang, A. M. Cypess, E. D. Muse, C.-R. Wu, C. G. Unson, R. B. Merrifield, and T. P. Sakmar (2001)
PNAS 98, 10102-10107
   Abstract »    Full Text »    PDF »


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