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Science 5 March 1993:
Vol. 259. no. 5100, pp. 1450 - 1453
DOI: 10.1126/science.8383879
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Articles

Science, Vol 259, Issue 5100, 1450-1453
Copyright © 1993 by American Association for the Advancement of Science

articles

Deficiency in rhabdomyosarcomas of a factor required for MyoD activity and myogenesis

SJ Tapscott, MJ Thayer, and H Weintraub

Fred Hutchinson Cancer Research Center, Seattle, WA 98104.

Rhabdomyosarcoma cells express the myogenic helix-loop-helix proteins of the MyoD family but do not differentiate into skeletal muscle cells. Gel shift and transient transfection assays revealed that MyoD in the rhabdomyosarcoma cells was capable of binding DNA but was relatively nonfunctional as a transcriptional activator. Heterokaryon formation with fibroblasts resulted in the restoration of transcriptional activation by MyoD and the differentiation of the rhabdomyosarcoma cells into skeletal muscle cells. These results suggest that rhabdomyosarcomas are deficient in a factor required for MyoD activity.


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