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Science 5 March 1993:
Vol. 259. no. 5100, pp. 1415 - 1420
DOI: 10.1126/science.8451638
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Articles

Science, Vol 259, Issue 5100, 1415-1420
Copyright © 1993 by American Association for the Advancement of Science

articles

Molecular matchmakers

A Sancar and JE Hearst

Department of Biochemistry and Biophysics, University of North Carolina School of Medicine, Chapel Hill 27599.

Molecular matchmakers are a class of proteins that use the energy released from the hydrolysis of adenosine triphosphate to cause a conformational change in one or both components of a DNA binding protein pair to promote formation of a metastable DNA-protein complex. After matchmaking the matchmaker dissociates from the complex, permitting the matched protein to engage in other protein-protein interactions to bring about the effector function. Matchmaking is most commonly used under circumstances that require targeted, high-avidity DNA binding without relying solely on sequence specificity. Molecular matchmaking is an extensively used mechanism in repair, replication, and transcription and most likely in recombination and transposition reactions, too.


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