Note to users. If you're seeing this message, it means that your browser cannot find this page's style/presentation instructions -- or possibly that you are using a browser that does not support current Web standards. Find out more about why this message is appearing, and what you can do to make your experience of our site the best it can be.

Site Tools

  • AAAS
  • Subscribe
  • Feedback

Site Search

Search Advanced

Science 26 February 1993:
Vol. 259. no. 5099, pp. 1314 - 1318
DOI: 10.1126/science.7680491
Prev | Table of Contents | Next

Articles

Science, Vol 259, Issue 5099, 1314-1318
Copyright © 1993 by American Association for the Advancement of Science

articles

Inhibition of Rev-mediated HIV-1 expression by an RNA binding protein encoded by the interferon-inducible 9-27 gene

P Constantoulakis, M Campbell, BK Felber, G Nasioulas, E Afonina, and GN Pavlakis

Human Retrovirus Section, National Cancer Institute-Frederick Cancer Research and Development Center, MD 21702.

Interferon inhibits expression of human immunodeficiency virus type-1 (HIV-1) through unknown mechanisms. A gene inducible by interferon-alpha (IFN-alpha) and interferon-gamma (IFN-gamma) was isolated by screening of a human complementary DNA library for proteins binding to the Rev-responsive element (RRE) of HIV-1. The product of this gene, RBP9-27, was shown to bind RNA in vitro and to inhibit HIV-1 expression after transfection into human cells. RBP9-27 primarily inhibited Rev-dependent posttranscriptional steps of viral gene expression. Thus, RBP9-27 is a cellular factor that antagonizes Rev function. These results suggest an interferon-induced antiviral mechanism operating through the induction of RNA binding proteins such as RBP9-27. Elucidation of RBP9-27 function may lead to a better understanding of the mechanism of interferon action during HIV-1 infection.


THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES:
CUGBP1 Is Required for IFNbeta-Mediated Induction of Dominant-Negative CEBPbeta and Suppression of SIV Replication in Macrophages.
J. M. Dudaronek, S. A. Barber, and J. E. Clements (2007)
J. Immunol. 179, 7262-7269
   Abstract »    Full Text »    PDF »
Norepinephrine Accelerates HIV Replication Via Protein Kinase A-Dependent Effects on Cytokine Production.
S. W. Cole, Y. D. Korin, J. L. Fahey, and J. A. Zack (1998)
J. Immunol. 161, 610-616
   Abstract »    Full Text »    PDF »
Involvement of the IRF-1 transcription factor in antiviral responses to interferons.
T Kimura, K Nakayama, J Penninger, M Kitagawa, H Harada, T Matsuyama, N Tanaka, R Kamijo, J Vilcek, T. Mak, et al. (1994)
Science 264, 1921-1924
   Abstract »    PDF »
An antiviral soluble form of the LDL receptor induced by interferon.
D. Fischer, N Tal, D Novick, S Barak, and M Rubinstein (1993)
Science 262, 250-253
   Abstract »    PDF »
Inhibition of viral replication by interferon-gamma-induced nitric oxide synthase.
G Karupiah, Q. Xie, R. Buller, C Nathan, C Duarte, and J. MacMicking (1993)
Science 261, 1445-1448
   Abstract »    PDF »


To Advertise     Find Products

Science. ISSN 0036-8075 (print), 1095-9203 (online)