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Science 19 February 1993:
Vol. 259. no. 5098, pp. 1134 - 1138
DOI: 10.1126/science.8438164
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Articles

Science, Vol 259, Issue 5098, 1134-1138
Copyright © 1993 by American Association for the Advancement of Science

articles

A complete second gut induced by transplanted micromeres in the sea urchin embryo

A Ransick and EH Davidson

Division of Biology, California Institute of Technology, Pasadena 91125.

Founder cells for most early lineages of the sea urchin embryo are probably specified through inductive intercellular interactions. It is shown here that a complete respecification of cell fate occurs when 16-cell stage micromeres from the vegetal pole of a donor embryo are implanted into the animal pole of an intact recipient embryo. Animal pole cells adjacent to the transplanted micromeres are respecified from presumptive ectoderm into vegetal plate founder cells. These induced vegetal plate cells express the entire battery of genes characteristic of the endogenous vegetal plate cells. The ectopic vegetal plate invaginates during gastrulation to form a second archenteron which differentiates properly into a tripartite gut, as shown by the spatial pattern of expression of an endoderm-specific marker gene. Thus, transplanted micromeres can signal neighboring cells to induce them to change their fate.


THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES:
From the Cover: Feature Article: Global regulatory logic for specification of an embryonic cell lineage.
P. Oliveri, Q. Tu, and E. H. Davidson (2008)
PNAS 105, 5955-5962
   Abstract »    Full Text »    PDF »
The Snail repressor is required for PMC ingression in the sea urchin embryo.
S.-Y. Wu and D. R. McClay (2007)
Development 134, 1061-1070
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A Raf/MEK/ERK signaling pathway is required for development of the sea urchin embryo micromere lineage through phosphorylation of the transcription factor Ets.
E. Rottinger, L. Besnardeau, and T. Lepage (2004)
Development 131, 1075-1087
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Isolation of pigment cell specific genes in the sea urchin embryo by differential macroarray screening.
C. Calestani, J. P. Rast, and E. H. Davidson (2003)
Development 130, 4587-4596
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T-brain homologue (HpTb) is involved in the archenteron induction signals of micromere descendant cells in the sea urchin embryo.
T. Fuchikami, K. Mitsunaga-Nakatsubo, S. Amemiya, T. Hosomi, T. Watanabe, D. Kurokawa, M. Kataoka, Y. Harada, N. Satoh, S. Kusunoki, et al. (2003)
Development 129, 5205-5216
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Regulatory gene networks and the properties of the developmental process.
E. H. Davidson, D. R. McClay, and L. Hood (2003)
PNAS 100, 1475-1480
   Abstract »    Full Text »    PDF »
A Genomic Regulatory Network for Development.
E. H. Davidson, J. P. Rast, P. Oliveri, A. Ransick, C. Calestani, C.-H. Yuh, T. Minokawa, G. Amore, V. Hinman, C. Arenas-Mena, et al. (2002)
Science 295, 1669-1678
   Abstract »    Full Text »    PDF »
LvNotch signaling plays a dual role in regulating the position of the ectoderm-endoderm boundary in the sea urchin embryo.
D. R. Sherwood and D. R. McClay (2001)
Development 128, 2221-2232
   Abstract »    Full Text »    PDF »
Ca(2+) in specification of vegetal cell fate in early sea urchin embryos.
I Yazaki (2001)
J. Exp. Biol. 204, 823-834
   Abstract »    PDF »
A micromere induction signal is activated by beta-catenin and acts through notch to initiate specification of secondary mesenchyme cells in the sea urchin embryo.
D. McClay, R. Peterson, R. Range, A. Winter-Vann, and M. Ferkowicz (2000)
Development 127, 5113-5122
   Abstract »    PDF »
A BMP pathway regulates cell fate allocation along the sea urchin animal-vegetal embryonic axis.
L. Angerer, D. Oleksyn, C. Logan, D. McClay, L Dale, and R. Angerer (2000)
Development 127, 1105-1114
   Abstract »    PDF »
The role of micromere signaling in Notch activation and mesoderm specification during sea urchin embryogenesis.
H. Sweet, P. Hodor, and C. Ettensohn (1999)
Development 126, 5255-5265
   Abstract »    PDF »
Spatially restricted expression of PlOtp, a Paracentrotus lividus orthopedia-related homeobox gene, is correlated with oral ectodermal patterning and skeletal morphogenesis in late-cleavage sea urchin embryos.
M Di Bernardo, S Castagnetti, D Bellomonte, P Oliveri, R Melfi, F Palla, and G Spinelli (1999)
Development 126, 2171-2179
   Abstract »    PDF »
Nuclear beta-catenin is required to specify vegetal cell fates in the sea urchin embryo.
C. Logan, J. Miller, M. Ferkowicz, and D. McClay (1999)
Development 126, 345-357
   Abstract »    PDF »
beta -Catenin is essential for patterning the maternally specified animal-vegetal axis in the sea urchin embryo.
A. H. Wikramanayake, L. Huang, and W. H. Klein (1998)
PNAS 95, 9343-9348
   Abstract »    Full Text »    PDF »
Underlying assumptions of developmental models.
R. J. Britten (1998)
PNAS 95, 9372-9377
   Abstract »    Full Text »    PDF »
Specification of cell fate in the sea urchin embryo: summary and some proposed mechanisms.
E. Davidson, R. Cameron, and A Ransick (1998)
Development 125, 3269-3290
   Abstract »    PDF »
GSK3beta/shaggy mediates patterning along the animal-vegetal axis of the sea urchin embryo.
F Emily-Fenouil, C Ghiglione, G Lhomond, T Lepage, and C Gache (1998)
Development 125, 2489-2498
   Abstract »    PDF »
Archenteron precursor cells can organize secondary axial structures in the sea urchin embryo.
H Benink, G Wray, and J Hardin (1997)
Development 124, 3461-3470
   Abstract »    PDF »
The allocation of early blastomeres to the ectoderm and endoderm is variable in the sea urchin embryo.
C. Logan and D. McClay (1997)
Development 124, 2213-2223
   Abstract »    PDF »
Disruption of gastrulation and oral-aboral ectoderm differentiation in the Lytechinus pictus embryo by a dominant/negative PDGF receptor.
R. Ramachandran, A. Wikramanayake, J. Uzman, V Govindarajan, and C. Tomlinson (1997)
Development 124, 2355-2364
   Abstract »    PDF »
Quantitative functional interrelations within the cis-regulatory system of the S. purpuratus Endo16 gene.
C. Yuh, J. Moore, and E. Davidson (1996)
Development 122, 4045-4056
   Abstract »    PDF »
Early gene expression along the animal-vegetal axis in sea urchin embryoids and grafted embryos.
C Ghiglione, F Emily-Fenouil, P Chang, and C Gache (1996)
Development 122, 3067-3074
   Abstract »    PDF »
Altering cell fates in sea urchin embryos by overexpressing SpOtx, an orthodenticle-related protein.
C. Mao, A. Wikramanayake, L Gan, C. Chuang, R. Summers, and W. Klein (1996)
Development 122, 1489-1498
   Abstract »    PDF »
Regulative capacity of the archenteron during gastrulation in the sea urchin.
D. McClay and C. Logan (1996)
Development 122, 607-616
   Abstract »    PDF »
A fate map of the vegetal plate of the sea urchin (Lytechinus variegatus) mesenchyme blastula.
S. Ruffins and C. Ettensohn (1996)
Development 122, 253-263
   Abstract »    PDF »
Micromeres are required for normal vegetal plate specification in sea urchin embryos.
A Ransick and E. Davidson (1995)
Development 121, 3215-3222
   Abstract »    PDF »
A sea urchin homologue of the chordate Brachyury (T) gene is expressed in the secondary mesenchyme founder cells.
Y Harada, H Yasuo, and N Satoh (1995)
Development 121, 2747-2754
   Abstract »    PDF »
Autonomous and non-autonomous differentiation of ectoderm in different sea urchin species.
A. Wikramanayake, B. Brandhorst, and W. Klein (1995)
Development 121, 1497-1505
   Abstract »    PDF »
Size regulation and morphogenesis: a cellular analysis of skeletogenesis in the sea urchin embryo.
C. Ettensohn and K. Malinda (1993)
Development 119, 155-167
   Abstract »    PDF »
Later embryogenesis: regulatory circuitry in morphogenetic fields.
E. Davidson (1993)
Development 118, 665-690
   Abstract »    PDF »


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