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Science 12 February 1993: Vol. 259. no. 5097, pp. 946 - 951 DOI: 10.1126/science.8438152
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Articles
Science, Vol 259, Issue 5097, 946-951
Copyright © 1993 by American Association for the Advancement of Science
Cloning the differences between two complex genomes
N Lisitsyn,
N Lisitsyn,
and
M Wigler
Cold Spring Harbor Laboratory, NY 11724.
The analysis of the differences between two complex genomes holds promise for the discovery of infectious agents and probes useful for genetic studies. A system was developed in which subtractive and kinetic enrichment was used to purify restriction endonuclease fragments present in one population of DNA fragments but not in another. Application of this method to DNA populations of reduced complexity ("representations") resulted in the isolation of probes to viral genomes present as single copies in human DNA, and probes that detect polymorphisms between two individuals. In principle, this system, called representational difference analysis (RDA), may also be used for isolating probes linked to sites of genomic rearrangements, whether occurring spontaneously and resulting in genetic disorders or cancer, or programmed during differentiation and development.
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- Detection of Differential Gene Expression in Biofilm-Forming versus Planktonic Populations of Staphylococcus aureus Using Micro-Representational-Difference Analysis.
- P. Becker, W. Hufnagle, G. Peters, and M. Herrmann (2001)
Appl. Envir. Microbiol.
67, 2958-2965
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- c-Myc Is a Critical Target for C/EBP{alpha} in Granulopoiesis.
- L. M. Johansen, A. Iwama, T. A. Lodie, K. Sasaki, D. W. Felsher, T. R. Golub, and D. G. Tenen (2001)
Mol. Cell. Biol.
21, 3789-3806
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- Genetic Alterations of Candidate Tumor Suppressor ING1 in Human Esophageal Squamous Cell Cancer.
- L. Chen, N. Matsubara, T. Yoshino, T. Nagasaka, N. Hoshizima, Y. Shirakawa, Y. Naomoto, H. Isozaki, K. Riabowol, and N. Tanaka (2001)
Cancer Res.
61, 4345-4349
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- Detection of Differentially Expressed Genes in HeLa Fibroblast Hybrids Using Subtractive Suppression Hybridization.
- S. Nishizuka, H. Tsujimoto, and E. J. Stanbridge (2001)
Cancer Res.
61, 4536-4540
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- Gene expression profiling using a novel method: amplified differential gene expression (ADGE).
- Z. J. Chen, H. Shen, and K. D. Tew (2001)
Nucleic Acids Res.
29, e46
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- Cutting Edge: FISP (IL-4-Induced Secreted Protein), a Novel Cytokine-Like Molecule Secreted by Th2 Cells.
- G. Schaefer, C. Venkataraman, and U. Schindler (2001)
J. Immunol.
166, 5859-5863
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- Directed Gap Closure in Large-Scale Sequencing Projects.
- M. Frohme, A. A. Camargo, C. Czink, A. Y. Matsukuma, A. J.G. Simpson, J. D. Hoheisel, and S. Verjovski-Almeida (2001)
Genome Res.
11, 901-903
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- Genomic Interspecies Microarray Hybridization: Rapid Discovery of Three Thousand Genes in the Maize Endophyte, Klebsiella pneumoniae 342, by Microarray Hybridization with Escherichia coli K-12 Open Reading Frames.
- Y. Dong, J. D. Glasner, F. R. Blattner, and E. W. Triplett (2001)
Appl. Envir. Microbiol.
67, 1911-1921
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- RNA: a method to specifically inhibit PCR amplification of known members of a multigene family by degenerate primers.
- P. S. T. Yuen, K. M. Brooks, and Y. Li (2001)
Nucleic Acids Res.
29, e31
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- NmeSI Restriction-Modification System Identified by Representational Difference Analysis of a Hypervirulent Neisseria meningitidis Strain.
- A. Bart, Y. Pannekoek, J. Dankert, and A. van der Ende (2001)
Infect. Immun.
69, 1816-1820
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- Identification of sample-specific sequences in mammalian cDNA and genomic DNA by the novel ligation-mediated subtraction (Limes).
- T. E. Hansen-Hagge, U. Trefzer, A. S. zu Reventlow, K. Kaltoft, and W. Sterry (2001)
Nucleic Acids Res.
29, e20
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- Diversity Arrays: a solid state technology for sequence information independent genotyping.
- D. Jaccoud, K. Peng, D. Feinstein, and A. Kilian (2001)
Nucleic Acids Res.
29, e25
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- Gene Expression Patterns Associated with the Metastatic Phenotype in Rodent and Human Tumors.
- A. Nestl, O. D. Von Stein, K. Zatloukal, W.-G. Thies, P. Herrlich, M. Hofmann, and J. P. Sleeman (2001)
Cancer Res.
61, 1569-1577
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- Infectious Disease Issues in Xenotransplantation.
- R. S. Boneva, T. M. Folks, and L. E. Chapman (2001)
Clin. Microbiol. Rev.
14, 1-14
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- Molecular Properties, Biology, and Clinical Implications of TT Virus, a Recently Identified Widespread Infectious Agent of Humans.
- M. Bendinelli, M. Pistello, F. Maggi, C. Fornai, G. Freer, and M. L. Vatteroni (2001)
Clin. Microbiol. Rev.
14, 98-113
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- Overexpression of the G-Protein Inwardly Rectifying Potassium Channel 1 (GIRK1) in Primary Breast Carcinomas Correlates with Axillary Lymph Node Metastasis.
- B. K. Stringer, A. G. Cooper, and S. B. Shepard (2001)
Cancer Res.
61, 582-588
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- Rearrangements of the DNA in Carbon Ion-Induced Mutants of Arabidopsis thaliana.
- N. Shikazono, A. Tanaka, H. Watanabe, and S. Tano (2001)
Genetics
157, 379-387
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- Application of Representational Difference Analysis to Genomic Fragments of Marek's Disease Virus.
- D. Endoh, K.-O. Cho, K. Tsukamoto, T. Morimura, Y. Kon, and M. Hayashi (2000)
J. Clin. Microbiol.
38, 4310-4314
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