Note to users. If you're seeing this message, it means that your browser cannot find this page's style/presentation instructions -- or possibly that you are using a browser that does not support current Web standards. Find out more about why this message is appearing, and what you can do to make your experience of our site the best it can be.


Science 12 February 1993:
Vol. 259. no. 5097, pp. 946 - 951
DOI: 10.1126/science.8438152

Articles

Science, Vol 259, Issue 5097, 946-951
Copyright © 1993 by American Association for the Advancement of Science


articles

Cloning the differences between two complex genomes

N Lisitsyn, N Lisitsyn, and M Wigler

Cold Spring Harbor Laboratory, NY 11724.

The analysis of the differences between two complex genomes holds promise for the discovery of infectious agents and probes useful for genetic studies. A system was developed in which subtractive and kinetic enrichment was used to purify restriction endonuclease fragments present in one population of DNA fragments but not in another. Application of this method to DNA populations of reduced complexity ("representations") resulted in the isolation of probes to viral genomes present as single copies in human DNA, and probes that detect polymorphisms between two individuals. In principle, this system, called representational difference analysis (RDA), may also be used for isolating probes linked to sites of genomic rearrangements, whether occurring spontaneously and resulting in genetic disorders or cancer, or programmed during differentiation and development.


THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES:
Changes in Gene and Protein Expression during Tomato Ripening -- Consequences for the Safety Assessment of New Crop Plant Varieties.
E.J. Kok, S.J. Lehesranta, J.P. van Dijk, J.R. Helsdingen, W.T.P. Dijksma, A.M.A. Van Hoef, K.M. Koistinen, S.O. Karenlampi, H.A. Kuiper, and J. Keijer (2008)
Food Science and Technology International 14, 503-518
   Abstract »    PDF »
The History of Tumor Virology.
R. T. Javier and J. S. Butel (2008)
Cancer Res. 68, 7693-7706
   Abstract »    Full Text »    PDF »
Identification of transcripts overexpressed during airway epithelium differentiation.
B. Chhin, J. T. Pham, L. El Zein, K. Kaiser, O. Merrot, and P. Bouvagnet (2008)
Eur. Respir. J. 32, 121-128
   Abstract »    Full Text »    PDF »
An analytical pipeline for genomic representations used for cytosine methylation studies.
R. F. Thompson, M. Reimers, B. Khulan, M. Gissot, T. A. Richmond, Q. Chen, X. Zheng, K. Kim, and J. M. Greally (2008)
Bioinformatics 24, 1161-1167
   Abstract »    Full Text »    PDF »
Mutations in the neutral sphingomyelinase gene SMPD3 implicate the ceramide pathway in human leukemias.
W. J. Kim, R. A. Okimoto, L. E. Purton, M. Goodwin, S. M. Haserlat, F. Dayyani, D. A. Sweetser, A. I. McClatchey, O. A. Bernard, A. T. Look, et al. (2008)
Blood 111, 4716-4722
   Abstract »    Full Text »    PDF »
BAF180 Is a Critical Regulator of p21 Induction and a Tumor Suppressor Mutated in Breast Cancer.
W. Xia, S. Nagase, A. G. Montia, S. M. Kalachikov, M. Keniry, T. Su, L. Memeo, H. Hibshoosh, and R. Parsons (2008)
Cancer Res. 68, 1667-1674
   Abstract »    Full Text »    PDF »
Fine Haplotype Structure of a Chromosome 17 Region in the Laboratory and Wild Mouse.
Z. Trachtulec, C. Vlcek, O. Mihola, S. Gregorova, V. Fotopulosova, and J. Forejt (2008)
Genetics 178, 1777-1784
   Abstract »    Full Text »    PDF »
Identification of a Novel Cancer-Testis Antigen CRT2 Frequently Expressed in Various Cancers Using Representational Differential Analysis.
E. Hayashi, Y. Matsuzaki, G. Hasegawa, T. Yaguchi, S. Kurihara, T. Fujita, T. Kageshita, M. Sano, and Y. Kawakami (2007)
Clin. Cancer Res. 13, 6267-6274
   Abstract »    Full Text »    PDF »
A hierarchical clustering method for estimating copy number variation.
B. Xing, C. M. T. Greenwood, and S. B. Bull (2007)
Biostat. 8, 632-653
   Abstract »    Full Text »    PDF »
Encapsidation Determinants Located Downstream of the Major Splice Donor in the Maedi-Visna Virus Leader Region.
H. Bjarnadottir, B. Gudmundsson, J. Gudnason, and J. J. Jonsson (2006)
J. Virol. 80, 11743-11755
   Abstract »    Full Text »    PDF »
Representational fragment amplification: exponential amplification of fragmented cDNA enables multimillion-fold expression testing..
G. D. Sgarlato and H. H. Sussman (2006)
Clin. Chem. 52, 2164-2168
   Full Text »    PDF »
C35 (C17orf37) is a novel tumor biomarker abundantly expressed in breast cancer..
E. E. Evans, A. D. Henn, A. Jonason, M. J. Paris, L. M. Schiffhauer, M. A. Borrello, E. S. Smith, D. M. Sahasrabudhe, and M. Zauderer (2006)
Mol. Cancer Ther. 5, 2919-2930
   Abstract »    Full Text »    PDF »
Doubling genome size without polyploidization: Dynamics of retrotransposition-driven genomic expansions in Oryza australiensis, a wild relative of rice.
B. Piegu, R. Guyot, N. Picault, A. Roulin, A. Saniyal, H. Kim, K. Collura, D. S. Brar, S. Jackson, R. A. Wing, et al. (2006)
Genome Res. 16, 1262-1269
   Abstract »    Full Text »    PDF »
Mouse genomic representational oligonucleotide microarray analysis: Detection of copy number variations in normal and tumor specimens.
B. Lakshmi, I. M. Hall, C. Egan, J. Alexander, A. Leotta, J. Healy, L. Zender, M. S. Spector, W. Xue, S. W. Lowe, et al. (2006)
PNAS 103, 11234-11239
   Abstract »    Full Text »    PDF »
Identification of Markers for Helicobacter pylori Strains Isolated from Children with Peptic Ulcer Disease by Suppressive Subtractive Hybridization.
M. Oleastro, L. Monteiro, P. Lehours, F. Megraud, and A. Menard (2006)
Infect. Immun. 74, 4064-4074
   Abstract »    Full Text »    PDF »
Molecular Cloning and Characterization of beta-Expansin Gene Related to Root Hair Formation in Barley.
M. Kwasniewski and I. Szarejko (2006)
Plant Physiology 141, 1149-1158
   Abstract »    Full Text »    PDF »
Primer Extension Enrichment Reaction (PEER): a new subtraction method for identification of genetic differences between biological specimens.
L. Ganova-Raeva, X. Zhang, F. Cao, H. Fields, and Y. Khudyakov (2006)
Nucleic Acids Res. 34, e76
   Abstract »    Full Text »    PDF »
Competitive metagenomic DNA hybridization identifies host-specific microbial genetic markers in cow fecal samples..
O. C. Shanks, J. W. Santo Domingo, R. Lamendella, C. A. Kelty, and J. E. Graham (2006)
Appl. Envir. Microbiol. 72, 4054-4060
   Abstract »    Full Text »    PDF »
Ovulation-selective genes: the generation and characterization of an ovulatory-selective cDNA library..
A Hourvitz, E Gershon, J D Hennebold, S Elizur, E Maman, C Brendle, E Y Adashi, and N Dekel (2006)
J. Endocrinol. 188, 531-548
   Abstract »    Full Text »    PDF »
Novel DNA Sequences from Natural Strains of the Nitrogen-Fixing Symbiotic Bacterium Sinorhizobium meliloti.
H. Guo, S. Sun, T. M. Finan, and J. Xu (2005)
Appl. Envir. Microbiol. 71, 7130-7138
   Abstract »    Full Text »    PDF »
Representational Difference Analysis and Real-Time PCR for Strain-Specific Quantification of Lactobacillus sobrius sp. nov..
S. R. Konstantinov, H. Smidt, and W. M. de Vos (2005)
Appl. Envir. Microbiol. 71, 7578-7581
   Abstract »    Full Text »    PDF »
Detection of DNA copy number alterations using penalized least squares regression.
T. Huang, B. Wu, P. Lizardi, and H. Zhao (2005)
Bioinformatics 21, 3811-3817
   Abstract »    Full Text »    PDF »
A novel transcriptome subtraction method for the detection of differentially expressed genes in highly complex eukaryotes.
L. Li, D. Techel, N. Gretz, and A. Hildebrandt (2005)
Nucleic Acids Res. 33, e136
   Abstract »    Full Text »    PDF »
Quantitative Analysis of Multi-Species Oral Biofilms by TaqMan Real-Time PCR.
N. Suzuki, A. Yoshida, and Y. Nakano (2005)
Clin. Med. Res. 3, 176-185
   Abstract »    Full Text »    PDF »
{delta}{rho}-Web, an online tool to assess composition similarity of individual nucleic acid sequences.
M. W. J. van Passel, A. C. M. Luyf, A. H. C. van Kampen, A. Bart, and A. van der Ende (2005)
Bioinformatics 21, 3053-3055
   Abstract »    Full Text »    PDF »
Dual roles of zygotic and maternal Scribble1 in neural migration and convergent extension movements in zebrafish embryos.
H. Wada, M. Iwasaki, T. Sato, I. Masai, Y. Nishiwaki, H. Tanaka, A. Sato, Y. Nojima, and H. Okamoto (2005)
Development 132, 2273-2285
   Abstract »    Full Text »    PDF »
Species-independent detection of RNA virus by representational difference analysis using non-ribosomal hexanucleotides for reverse transcription.
D. Endoh, T. Mizutani, R. Kirisawa, Y. Maki, H. Saito, Y. Kon, S. Morikawa, and M. Hayashi (2005)
Nucleic Acids Res. 33, e65
   Abstract »    Full Text »    PDF »
Identification of the mismatch repair genes PMS2 and MLH1 as p53 target genes by using serial analysis of binding elements.
J. Chen and I. Sadowski (2005)
PNAS 102, 4813-4818
   Abstract »    Full Text »    PDF »
Expression of the Putative Sterol Binding Protein Stard6 Gene Is Male Germ Cell Specific.
C. Gomes, S.-D. Oh, J.-W. Kim, S.-Y. Chun, K. Lee, H.-B. Kwon, and J. Soh (2005)
Biol Reprod 72, 651-658
   Abstract »    Full Text »    PDF »
Identification and validation of cell surface antigens for antibody targeting in oncology.
P. Carter, L. Smith, and M. Ryan (2004)
Endocr. Relat. Cancer 11, 659-687
   Abstract »    Full Text »    PDF »
Gene Expression Profiling of Embryonic Stem Cells Leads to Greater Understanding of Pluripotency and Early Developmental Events.
R. R. Rao and S. L. Stice (2004)
Biol Reprod 71, 1772-1778
   Abstract »    Full Text »    PDF »
Identification of Cyclin D3 as a Direct Target of E2A Using DamID.
S. Song, J. Cooperman, D. L. Letting, G. A. Blobel, and J. K. Choi (2004)
Mol. Cell. Biol. 24, 8790-8802
   Abstract »    Full Text »    PDF »
Lysyl Oxidase Is a Tumor Suppressor Gene Inactivated by Methylation and Loss of Heterozygosity in Human Gastric Cancers.
A. Kaneda, K. Wakazono, T. Tsukamoto, N. Watanabe, Y. Yagi, M. Tatematsu, M. Kaminishi, T. Sugimura, and T. Ushijima (2004)
Cancer Res. 64, 6410-6415
   Abstract »    Full Text »    PDF »
An in vitro strategy for the selective isolation of anomalous DNA from prokaryotic genomes.
M. W. J. van Passel, A. Bart, R. J. A. Waaijer, A. C. M. Luyf, A. H. C. van Kampen, and A. van der Ende (2004)
Nucleic Acids Res. 32, e114
   Abstract »    Full Text »    PDF »
Real-Time TaqMan PCR for Quantifying Oral Bacteria during Biofilm Formation.
N. Suzuki, Y. Nakano, A. Yoshida, Y. Yamashita, and Y. Kiyoura (2004)
J. Clin. Microbiol. 42, 3827-3830
   Abstract »    Full Text »    PDF »
Large-Scale Copy Number Polymorphism in the Human Genome.
J. Sebat, B. Lakshmi, J. Troge, J. Alexander, J. Young, P. Lundin, S. Maner, H. Massa, M. Walker, M. Chi, et al. (2004)
Science 305, 525-528
   Abstract »    Full Text »    PDF »
Identification of chaperonin CCT{gamma} subunit as a determinant of retinotectal development by whole-genome subtraction cloning from zebrafish no tectal neuron mutant.
N. Matsuda and M. Mishina (2004)
Development 131, 1913-1925
   Abstract »    Full Text »    PDF »
Hypermethylation and Silencing of the Putative Tumor Suppressor Tazarotene-Induced Gene 1 in Human Cancers.
E. M. Youssef, X.-q. Chen, E. Higuchi, Y. Kondo, G. Garcia-Manero, R. Lotan, and J.-P. J. Issa (2004)
Cancer Res. 64, 2411-2417
   Abstract »    Full Text »    PDF »
A novel method for screening species-specific gDNA probes for species identification.
T. X. Li, J. Wang, Y. Bai, X. Sun, and Z. Lu (2004)
Nucleic Acids Res. 32, e45
   Abstract »    Full Text »    PDF »
A 38-Kilobase Pathogenicity Island Specific for Mycobacterium avium subsp. paratuberculosis Encodes Cell Surface Proteins Expressed in the Host.
J. Stratmann, B. Strommenger, R. Goethe, K. Dohmann, G.-F. Gerlach, K. Stevenson, L.-l. Li, Q. Zhang, V. Kapur, and T. J. Bull (2004)
Infect. Immun. 72, 1265-1274
   Abstract »    Full Text »    PDF »
Isolation of Y Chromosome-specific Microsatellites in the Horse and Cross-species Amplification in the Genus Equus.
B. Wallner, F. Piumi, G. Brem, M. Muller, and R. Achmann (2004)
J. Hered. 95, 158-164
   Abstract »    Full Text »    PDF »
Two-dimensional conformation-dependent electrophoresis (2D-CDE) to separate DNA fragments containing unmatched bulge from complex DNA samples.
G. H. Gunnarsson, H. G. Thormar, B. Gudmundsson, L. Akesson, and J. J. Jonsson (2004)
Nucleic Acids Res. 32, e23
   Abstract »    Full Text »    PDF »
High throughput gene expression profiling: a molecular approach to integrative physiology.
M. Liang, A. W. Cowley Jr, and A. S. Greene (2004)
J. Physiol. 554, 22-30
   Abstract »    Full Text »    PDF »
Identification of Dimethyl Sulfoxide Reductase in Actinobacillus pleuropneumoniae and Its Role in Infection.
N. Baltes, I. Hennig-Pauka, I. Jacobsen, A. D. Gruber, and G. F. Gerlach (2003)
Infect. Immun. 71, 6784-6792
   Abstract »    Full Text »    PDF »
Subtractive Hybridization Identifies a Novel Predicted Protein Mediating Epithelial Cell Invasion by Virulent Serotype III Group B Streptococcus agalactiae.
E. E. Adderson, S. Takahashi, Y. Wang, J. Armstrong, D. V. Miller, and J. F. Bohnsack (2003)
Infect. Immun. 71, 6857-6863
   Abstract »    Full Text »    PDF »
Characterization of Genetic Differences between Mycobacterium avium subsp. paratuberculosis Type I and Type II Isolates.
K. Dohmann, B. Strommenger, K. Stevenson, L. de Juan, J. Stratmann, V. Kapur, T. J. Bull, and G.-F. Gerlach (2003)
J. Clin. Microbiol. 41, 5215-5223
   Abstract »    Full Text »    PDF »
Genome-wide Identification of Chromosomal Regions of Increased Tumor Expression by Transcriptome Analysis.
Y. Zhou, S.-M. Luoh, Y. Zhang, C. Watanabe, T. D. Wu, M. Ostland, W. I. Wood, and Z. Zhang (2003)
Cancer Res. 63, 5781-5784
   Abstract »    Full Text »    PDF »
Clusterin as a biomarker in murine and human intestinal neoplasia.
X. Chen, R. B. Halberg, W. M. Ehrhardt, J. Torrealba, and W. F. Dove (2003)
PNAS 100, 9530-9535
   Abstract »    Full Text »    PDF »
Overexpression of FABP7 in Down syndrome fetal brains is associated with PKNOX1 gene-dosage imbalance.
M. F. Sanchez-Font, A. Bosch-Comas, R. Gonzalez-Duarte, and G. Marfany (2003)
Nucleic Acids Res. 31, 2769-2777
   Abstract »    Full Text »    PDF »
Analysis of 4.3 Kilobases of Divergent Locus B of Macaque Retroperitoneal Fibromatosis-Associated Herpesvirus Reveals a Close Similarity in Gene Sequence and Genome Organization to Kaposi's Sarcoma-Associated Herpesvirus.
T. M. Rose, J. T. Ryan, E. R. Schultz, B. W. Raden, and C.-C. Tsai (2003)
J. Virol. 77, 5084-5097
   Abstract »    Full Text »    PDF »
Formation of Solo-LTRs Through Unequal Homologous Recombination Counterbalances Amplifications of LTR Retrotransposons in Rice Oryza sativa L..
C. Vitte and O. Panaud (2003)
Mol. Biol. Evol. 20, 528-540
   Abstract »    Full Text »    PDF »
Gene expression profiling of preneoplastic liver disease and liver cancer: a new era for improved early detection and treatment of these deadly diseases?.
J. W. Kim and X. W. Wang (2003)
Carcinogenesis 24, 363-369
   Abstract »    Full Text »    PDF »
Screening of Gene-Associated Polymorphisms by Use of In-Gel Competitive Reassociation and EST (cDNA) Array Hybridization.
K. Gotoh and M. Oishi (2003)
Genome Res. 13, 492-495
   Abstract »    Full Text »    PDF »
Small Unstable Apoptotic Protein, an Apoptosis-associated Protein, Suppresses Proliferation of Myeloid Cells.
S. J. Baker (2003)
Cancer Res. 63, 705-712
   Abstract »    Full Text »    PDF »
Appraisal of genetic and epigenetic congruity of a monozygotic twin pair discordant for schizophrenia.
P McDonald, M Lewis, B Murphy, R O'Reilly, and S M Singh (2003)
J. Med. Genet. 40, e16-16
   Full Text »    PDF »
LSSIG is a novel murine leukocyte-specific GPCR that is induced by the activation of STAT3.
T. Senga, S. Iwamoto, T. Yoshida, T. Yokota, K. Adachi, E. Azuma, M. Hamaguchi, and T. Iwamoto (2003)
Blood 101, 1185-1187
   Abstract »    Full Text »    PDF »
Paired-like Repression/Activation in Pituitary Development.
L. E. Olson, J. S. Dasen, B. Gun Ju, J. Tollkuhn, and M. G. Rosenfeld (2003)
Recent Prog. Horm. Res. 58, 249-261
   Abstract »    Full Text »    PDF »
Digital karyotyping.
T.-L. Wang, C. Maierhofer, M. R. Speicher, C. Lengauer, B. Vogelstein, K. W. Kinzler, and V. E. Velculescu (2002)
PNAS 99, 16156-16161
   Abstract »    Full Text »    PDF »
Cloning and Analysis of the Thrombopoietin-induced Megakaryocyte-specific Glycoprotein VI Promoter and Its Regulation by GATA-1, Fli-1, and Sp1.
M. L. Holmes, N. Bartle, M. Eisbacher, and B. H. Chong (2002)
J. Biol. Chem. 277, 48333-48341
   Abstract »    Full Text »    PDF »
Identification of Genes That Are Downregulated in the Absence of the POU Domain Transcription Factor pou3f1 (Oct-6, Tst-1, SCIP) in Sciatic Nerve.
J. R. Bermingham Jr, S. Shumas, T. Whisenhunt, E. E. Sirkowski, S. O'Connell, S. S. Scherer, and M. G. Rosenfeld (2002)
J. Neurosci. 22, 10217-10231
   Abstract »    Full Text »    PDF »
Identification of Silencing of Nine Genes in Human Gastric Cancers.
A. Kaneda, M. Kaminishi, K. Yanagihara, T. Sugimura, and T. Ushijima (2002)
Cancer Res. 62, 6645-6650
   Abstract »    Full Text »    PDF »
Representational Difference Analysis between Afa/Dr Diffusely Adhering Escherichia coli and Nonpathogenic E. coli K-12.
A.-B. Blanc-Potard, C. Tinsley, I. Scaletsky, C. Le Bouguenec, J. Guignot, A. L. Servin, X. Nassif, and M.-F. Bernet-Camard (2002)
Infect. Immun. 70, 5503-5511
   Abstract »    Full Text »    PDF »
Isolation and Characterization of Triacontanol-Regulated Genes in Rice (Oryza sativa L.): Possible Role of Triacontanol as a Plant Growth Stimulator.
X. Chen, H. Yuan, R. Chen, L. Zhu, B. Du, Q. Weng, and G. He (2002)
Plant Cell Physiol. 43, 869-876
   Abstract »    Full Text »    PDF »
NotI subtraction and NotI-specific microarrays to detect copy number and methylation changes in whole genomes.
J. Li, A. Protopopov, F. Wang, V. Senchenko, V. Petushkov, O. Vorontsova, L. Petrenko, V. Zabarovska, O. Muravenko, E. Braga, et al. (2002)
PNAS 99, 10724-10729
   Abstract »    Full Text »    PDF »
An approach for global scanning of single nucleotide variations.
X. Pan and S. M. Weissman (2002)
PNAS 99, 9346-9351
   Abstract »    Full Text »    PDF »
Profiling and selection of genes differentially expressed in the pylorus of rat strains with different proliferative responses and stomach cancer susceptibility.
S. Yamashita, K. Wakazono, T. Sugimura, and T. Ushijima (2002)
Carcinogenesis 23, 923-928
   Abstract »    Full Text »    PDF »
Plunc, a Member of the Secretory Gland Protein Family, Is Up-regulated in Nasal Respiratory Epithelium after Olfactory Bulbectomy.
Y. K. Sung, C. Moon, J.-Y. Yoo, C. Moon, D. Pearse, J. Pevsner, and G. V. Ronnett (2002)
J. Biol. Chem. 277, 12762-12769
   Abstract »    Full Text »    PDF »
Opposing Roles of the Extracellular Signal-Regulated Kinase and p38 Mitogen-Activated Protein Kinase Cascades in Ras-Mediated Downregulation of Tropomyosin.
J. M. Shields, H. Mehta, K. Pruitt, and C. J. Der (2002)
Mol. Cell. Biol. 22, 2304-2317
   Abstract »    Full Text »    PDF »
Methylation Matters: Modeling a Manageable Genome.
J. M. Ordway and T. Curran (2002)
Cell Growth Differ. 13, 149-162
   Full Text »    PDF »
Application of gene expression profiling to cardiovascular disease.
P.A Henriksen and Y Kotelevtsev (2002)
Cardiovasc Res 54, 16-24
   Abstract »    Full Text »    PDF »
Representational difference analysis in a lupus-prone mouse strain results in the identification of an unstable region of the genome on chromosome 11.
G. Fu, M. E. K. Haywood, and B. J. Morley (2002)
Nucleic Acids Res. 30, 1394-1400
   Abstract »    Full Text »    PDF »
Loss of Expression of GADD45{gamma}, a Growth Inhibitory Gene, in Human Pituitary Adenomas: Implications for Tumorigenesis.
X. Zhang, H. Sun, D. C. Danila, S. R. Johnson, Y. Zhou, B. Swearingen, and A. Klibanski (2002)
J. Clin. Endocrinol. Metab. 87, 1262-1267
   Abstract »    Full Text »    PDF »
Subtraction-coupled Custom Microarray Analysis for Gene Discovery and Gene Expression Studies in the CNS.
J. D. Dougherty and D. H. Geschwind (2002)
Chem Senses 27, 293-298
   Abstract »    Full Text »    PDF »
Upstream binding factor up-regulated in hepatocellular carcinoma is related to the survival and cisplatin-sensitivity of cancer cells.
R. HUANG, T. WU, L. XU, A. LIU, Y. JI, and G. HU (2002)
FASEB J 16, 293-301
   Abstract »    Full Text »    PDF »
Identification of Virulence Genes in a Pathogenic Strain of Pseudomonas aeruginosa by Representational Difference Analysis.
J. Y. Choi, C. D. Sifri, B. C. Goumnerov, L. G. Rahme, F. M. Ausubel, and S. B. Calderwood (2002)
J. Bacteriol. 184, 952-961
   Abstract »    Full Text »    PDF »
Mapping of Sex Determination Loci on the White Campion (Silene latifolia) Y Chromosome Using Amplified Fragment Length Polymorphism.
S. Lebel-Hardenack, E. Hauser, T. F. Law, J. Schmid, and S. R. Grant (2002)
Genetics 160, 717-725
   Abstract »    Full Text »    PDF »
Sexually dimorphic development of mouse primordial germ cells: switching from oogenesis to spermatogenesis.
I. R. Adams and A. McLaren (2002)
Development 129, 1155-1164
   Abstract »    Full Text »    PDF »
Cloning and Characterization of a p53-related Protein Kinase Expressed in Interleukin-2-activated Cytotoxic T-cells, Epithelial Tumor Cell Lines, and the Testes.
Y. Abe, S. Matsumoto, S. Wei, K. Nezu, A. Miyoshi, K. Kito, N. Ueda, K. Shigemoto, Y. Hitsumoto, J.-i. Nikawa, et al. (2001)
J. Biol. Chem. 276, 44003-44011
   Abstract »    Full Text »    PDF »
Isolation of X and Y Chromosome-Specific DNA Markers From a Liverwort, Marchantia polymorpha, by Representational Difference Analysis.
M. Fujisawa, K. Hayashi, T. Nishio, T. Bando, S. Okada, K. T. Yamato, H. Fukuzawa, and K. Ohyama (2001)
Genetics 159, 981-985
   Abstract »    Full Text »    PDF »
A novel method to isolate the common fraction of two DNA samples: hybrid specific amplification (HSA).
F. Lecerf, L. Foggia, P. Mulsant, A. Bonnet, and F. Hatey (2001)
Nucleic Acids Res. 29, e87
   Abstract »    Full Text »    PDF »
A critical evaluation of differential display as a tool to identify genes involved in legume nodulation: looking back and looking forward.
S. Lievens, S. Goormachtig, and M. Holsters (2001)
Nucleic Acids Res. 29, 3459-3468
   Abstract »    Full Text »    PDF »
Whipple's Disease and ""Tropheryma whippelii"".
F. Dutly and M. Altwegg (2001)
Clin. Microbiol. Rev. 14, 561-583
   Abstract »    Full Text »    PDF »
Detection of Differential Gene Expression in Biofilm-Forming versus Planktonic Populations of Staphylococcus aureus Using Micro-Representational-Difference Analysis.
P. Becker, W. Hufnagle, G. Peters, and M. Herrmann (2001)
Appl. Envir. Microbiol. 67, 2958-2965
   Abstract »    Full Text »    PDF »
c-Myc Is a Critical Target for C/EBP{alpha} in Granulopoiesis.
L. M. Johansen, A. Iwama, T. A. Lodie, K. Sasaki, D. W. Felsher, T. R. Golub, and D. G. Tenen (2001)
Mol. Cell. Biol. 21, 3789-3806
   Abstract »    Full Text »
Genetic Alterations of Candidate Tumor Suppressor ING1 in Human Esophageal Squamous Cell Cancer.
L. Chen, N. Matsubara, T. Yoshino, T. Nagasaka, N. Hoshizima, Y. Shirakawa, Y. Naomoto, H. Isozaki, K. Riabowol, and N. Tanaka (2001)
Cancer Res. 61, 4345-4349
   Abstract »    Full Text »    PDF »
Detection of Differentially Expressed Genes in HeLa Fibroblast Hybrids Using Subtractive Suppression Hybridization.
S. Nishizuka, H. Tsujimoto, and E. J. Stanbridge (2001)
Cancer Res. 61, 4536-4540
   Abstract »    Full Text »    PDF »
Gene expression profiling using a novel method: amplified differential gene expression (ADGE).
Z. J. Chen, H. Shen, and K. D. Tew (2001)
Nucleic Acids Res. 29, e46
   Abstract »    Full Text »    PDF »
Cutting Edge: FISP (IL-4-Induced Secreted Protein), a Novel Cytokine-Like Molecule Secreted by Th2 Cells.
G. Schaefer, C. Venkataraman, and U. Schindler (2001)
J. Immunol. 166, 5859-5863
   Abstract »    Full Text »    PDF »
Directed Gap Closure in Large-Scale Sequencing Projects.
M. Frohme, A. A. Camargo, C. Czink, A. Y. Matsukuma, A. J.G. Simpson, J. D. Hoheisel, and S. Verjovski-Almeida (2001)
Genome Res. 11, 901-903
   Abstract »    Full Text »
Genomic Interspecies Microarray Hybridization: Rapid Discovery of Three Thousand Genes in the Maize Endophyte, Klebsiella pneumoniae 342, by Microarray Hybridization with Escherichia coli K-12 Open Reading Frames.
Y. Dong, J. D. Glasner, F. R. Blattner, and E. W. Triplett (2001)
Appl. Envir. Microbiol. 67, 1911-1921
   Abstract »    Full Text »
RNA: a method to specifically inhibit PCR amplification of known members of a multigene family by degenerate primers.
P. S. T. Yuen, K. M. Brooks, and Y. Li (2001)
Nucleic Acids Res. 29, e31
   Abstract »    Full Text »    PDF »
NmeSI Restriction-Modification System Identified by Representational Difference Analysis of a Hypervirulent Neisseria meningitidis Strain.
A. Bart, Y. Pannekoek, J. Dankert, and A. van der Ende (2001)
Infect. Immun. 69, 1816-1820
   Abstract »    Full Text »    PDF »
Identification of sample-specific sequences in mammalian cDNA and genomic DNA by the novel ligation-mediated subtraction (Limes).
T. E. Hansen-Hagge, U. Trefzer, A. S. zu Reventlow, K. Kaltoft, and W. Sterry (2001)
Nucleic Acids Res. 29, e20
   Abstract »    Full Text »    PDF »
Diversity Arrays: a solid state technology for sequence information independent genotyping.
D. Jaccoud, K. Peng, D. Feinstein, and A. Kilian (2001)
Nucleic Acids Res. 29, e25
   Abstract »    Full Text »    PDF »
Gene Expression Patterns Associated with the Metastatic Phenotype in Rodent and Human Tumors.
A. Nestl, O. D. Von Stein, K. Zatloukal, W.-G. Thies, P. Herrlich, M. Hofmann, and J. P. Sleeman (2001)
Cancer Res. 61, 1569-1577
   Abstract »    Full Text »
Infectious Disease Issues in Xenotransplantation.
R. S. Boneva, T. M. Folks, and L. E. Chapman (2001)
Clin. Microbiol. Rev. 14, 1-14
   Abstract »    Full Text »    PDF »
Molecular Properties, Biology, and Clinical Implications of TT Virus, a Recently Identified Widespread Infectious Agent of Humans.
M. Bendinelli, M. Pistello, F. Maggi, C. Fornai, G. Freer, and M. L. Vatteroni (2001)
Clin. Microbiol. Rev. 14, 98-113
   Abstract »    Full Text »    PDF »
Overexpression of the G-Protein Inwardly Rectifying Potassium Channel 1 (GIRK1) in Primary Breast Carcinomas Correlates with Axillary Lymph Node Metastasis.
B. K. Stringer, A. G. Cooper, and S. B. Shepard (2001)
Cancer Res. 61, 582-588
   Abstract »    Full Text »
Rearrangements of the DNA in Carbon Ion-Induced Mutants of Arabidopsis thaliana.
N. Shikazono, A. Tanaka, H. Watanabe, and S. Tano (2001)
Genetics 157, 379-387
   Abstract »    Full Text »
Application of Representational Difference Analysis to Genomic Fragments of Marek's Disease Virus.
D. Endoh, K.-O. Cho, K. Tsukamoto, T. Morimura, Y. Kon, and M. Hayashi (2000)
J. Clin. Microbiol. 38, 4310-4314
   Abstract »    Full Text »



To Advertise     Find Products


Science. ISSN 0036-8075 (print), 1095-9203 (online)