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Science 22 January 1993:
Vol. 259. no. 5094, pp. 508 - 510
DOI: 10.1126/science.259.5094.508
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Articles

Stress Protection of Transgenic Tobacco by Production of the Osmolyte Mannitol

Mitchell C. Tarczynski 1, Richard G. Jensen 1, and Hans J. Bohnert 1

1 Department of Biochemistry, University of Arizona, Tucson, AZ 85721

Correspondence should be addressed at 5 Prime rarr 3 Prime, Inc., 5603 Arapahoe, Boulder, CO 80303

The accumulation of sugar alcohols and other low molecular weight metabolites such as proline and glycine-betaine is a widespread response that may protect against environmental stress that occurs in a diverse range of organisms. Transgenic tobacco plants that synthesize and accumulate the sugar alcohol mannitol were engineered by introduction of a bacterial gene that encodes mannitol 1 -phosphate dehydrogenase. Growth of plants from control and mannitol-containing lines in the absence and presence of added sodium chloride was analyzed. Plants containing mannitol had an increased ability to tolerate high salinity.

Submitted on July 8, 1992
Accepted on November 6, 1992


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