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Science 8 January 1993:
Vol. 259. no. 5092, pp. 234 - 238
DOI: 10.1126/science.8421784
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Articles

Science, Vol 259, Issue 5092, 234-238
Copyright © 1993 by American Association for the Advancement of Science

articles

Rate and mechanism of nonhomologous recombination during a single cycle of retroviral replication

J Zhang and HM Temin

McArdle Laboratory for Cancer Research, University of Wisconsin-Madison 53706.

Oncogenes discovered in retroviruses such as Rous sarcoma virus were generated by transduction of cellular proto-oncogenes into the viral genome. Several different kinds of junctions between the viral and proto-oncogene sequences have been found in different viruses. A system of retrovirus vectors and a protocol that mimicked this transduction during a single cycle of retrovirus replication was developed. The transduction involved the formation of a chimeric viral-cellular RNA, strand switching of the reverse transcription growing point from an infectious retrovirus to the chimeric RNA, and often a subsequent deletion during the rest of viral DNA synthesis. A short region of sequence identity was frequently used for the strand switching. The rate of this process was about 0.1 to 1 percent of the rate of homologous retroviral recombination.


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