Note to users. If you're seeing this message, it means that your browser cannot find this page's style/presentation instructions -- or possibly that you are using a browser that does not support current Web standards. Find out more about why this message is appearing, and what you can do to make your experience of our site the best it can be.

Site Tools

  • AAAS
  • Subscribe
  • Feedback

Site Search

Search Advanced

Science 8 January 1993:
Vol. 259. no. 5092, pp. 224 - 227
DOI: 10.1126/science.7678469
Prev | Table of Contents | Next

Articles

Science, Vol 259, Issue 5092, 224-227
Copyright © 1993 by American Association for the Advancement of Science

articles

Presentation of a viral T cell epitope expressed in the CDR3 region of a self immunoglobulin molecule

H Zaghouani, R Steinman, R Nonacs, H Shah, W Gerhard, and C Bona

Department of Microbiology, Mount Sinai School of Medicine, New York, NY 10029.

Synthetic peptides corresponding to microbial epitopes stimulate T cell immunity but their immunogenicity is poor and their half-lives are short. A viral epitope inserted into the complementarity-determining region 3 (CDR3) loop of the heavy chain of a self immunoglobulin (Ig) molecule was generated from the Ig context and was presented by I-Ed class II molecules to virus-specific, CD4+ T cells. Chimeric Ig-peptide was presented 100 to 1000 times more efficiently than free synthetic peptide and was able to prime virus-specific T cells in vivo. These features suggest that antigenized Ig can provide an improved and safe vaccine for the presentation of microbial and other peptides.


THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES:
Recombinant antibodies for delivery of antigen: a single loop between {beta}-strands in the constant region can accommodate long, complex and tandem T cell epitopes.
G. Tunheim, K. W. Schjetne, I. B. Rasmussen, L. M. Sollid, I. Sandlie, and B. Bogen (2008)
Int. Immunol. 20, 295-306
   Abstract »    Full Text »    PDF »
Immunologic Control of Tumors by In Vivo Fc{gamma} Receptor-Targeted Antigen Loading in Conjunction with Double-Stranded RNA-Mediated Immune Modulation.
A. Bot, D. Smith, B. Phillips, S. Bot, C. Bona, and H. Zaghouani (2006)
J. Immunol. 176, 1363-1374
   Abstract »    Full Text »    PDF »
Human CD14 is an efficient target for recombinant immunoglobulin vaccine constructs that deliver T cell epitopes.
G. Tunheim, K. W. Schjetne, A. B. Fredriksen, I. Sandlie, and B. Bogen (2005)
J. Leukoc. Biol. 77, 303-310
   Abstract »    Full Text »    PDF »
Break of Neonatal Th1 Tolerance and Exacerbation of Experimental Allergic Encephalomyelitis by Interference with B7 Costimulation.
J. J. Bell, B. Min, R. K. Gregg, H.-H. Lee, and H. Zaghouani (2003)
J. Immunol. 171, 1801-1808
   Abstract »    Full Text »    PDF »
Infection-Triggered Regulatory Mechanisms Override the Role of STAT 4 in Control of the Immune Response to Influenza Virus Antigens.
A. Bot, E. Rodrigo, T. Wolfe, S. Bot, and M. G. Von Herrath (2003)
J. Virol. 77, 5794-5800
   Abstract »    Full Text »    PDF »
A mouse C{kappa}-specific T cell clone indicates that DC-SIGN is an efficient target for antibody-mediated delivery of T cell epitopes for MHC class II presentation.
K. W. Schjetne, K. M. Thompson, T. Aarvak, B. Fleckenstein, L. M. Sollid, and B. Bogen (2002)
Int. Immunol. 14, 1423-1430
   Abstract »    Full Text »    PDF »
On the Role of Dendritic Cells in Peripheral T Cell Tolerance and Modulation of Autoimmunity.
K. L. Legge, R. K. Gregg, R. Maldonado-Lopez, L. Li, J. C. Caprio, M. Moser, and H. Zaghouani (2002)
J. Exp. Med. 196, 217-227
   Abstract »    Full Text »    PDF »
Efficient Delivery of T Cell Epitopes to APC by Use of MHC Class II-Specific Troybodies.
E. Lunde, K. H. Western, I. B. Rasmussen, I. Sandlie, and B. Bogen (2002)
J. Immunol. 168, 2154-2162
   Abstract »    Full Text »    PDF »
Neonatal Exposure to Antigen Primes the Immune System to Develop Responses in Various Lymphoid Organs and Promotes Bystander Regulation of Diverse T Cell Specificities.
C. D. Pack, A. E. Cestra, B. Min, K. L. Legge, L. Li, J. C. Caprio-Young, J. J. Bell, R. K. Gregg, and H. Zaghouani (2001)
J. Immunol. 167, 4187-4195
   Abstract »    Full Text »    PDF »
Neonatal Immunity Develops in a Transgenic TCR Transfer Model and Reveals a Requirement for Elevated Cell Input to Achieve Organ-Specific Responses.
L. Li, K. L. Legge, B. Min, J. J. Bell, R. Gregg, J. Caprio, and H. Zaghouani (2001)
J. Immunol. 167, 2585-2594
   Abstract »    Full Text »    PDF »
IL-2 linked to a peptide from influenza hemagglutinin enhances T cell activation by affecting the antigen-presentation function of bone marrow-derived dendritic cells.
L. Faulkner, G. Buchan, E. Lockhart, L. Slobbe, M. Wilson, and M. Baird (2001)
Int. Immunol. 13, 713-721
   Abstract »    Full Text »    PDF »
Successful Interference with Cellular Immune Responses to Immunogenic Proteins Encoded by Recombinant Viral Vectors.
A. Sarukhan, S. Camugli, B. Gjata, H. von Boehmer, O. Danos, and K. Jooss (2001)
J. Virol. 75, 269-277
   Abstract »    Full Text »
Coupling of Peripheral Tolerance to Endogenous Interleukin 10 Promotes Effective Modulation of Myelin-activated T Cells and Ameliorates Experimental Allergic Encephalomyelitis.
K. L. Legge, B. Min, J. J. Bell, J. C. Caprio, L. Li, R. K. Gregg, and H. Zaghouani (2000)
J. Exp. Med. 191, 2039-2052
   Abstract »    Full Text »    PDF »
Phage-Displayed T-Cell Epitope Grafted into Immunoglobulin Heavy-Chain Complementarity-Determining Regions: an Effective Vaccine Design Tested in Murine Cysticercosis.
K. Manoutcharian, L. I. Terrazas, G. Gevorkian, G. Acero, P. Petrossian, M. Rodriguez, and T. Govezensky (1999)
Infect. Immun. 67, 4764-4770
   Abstract »    Full Text »    PDF »
Epitope-Specific Antibody and Suppression of Autoantibody Responses Against a Hybrid Self Protein.
G. L. Lohnas, S. F. Roberts, A. Pilon, and A. Tramontano (1998)
J. Immunol. 161, 6518-6525
   Abstract »    Full Text »    PDF »
Neonatal Exposure to a Self-Peptide-Immunoglobulin Chimera Circumvents the Use of Adjuvant and Confers Resistance to Autoimmune Disease by a Novel Mechanism Involving Interleukin 4 Lymph Node Deviation and Interferon gamma -mediated Splenic Anergy.
B. Min, K. L. Legge, C. Pack, and H. Zaghouani (1998)
J. Exp. Med. 188, 2007-2017
   Abstract »    Full Text »    PDF »
TCR Agonist and Antagonist Exert In Vivo Cross-Regulation When Presented on Igs.
K. L. Legge, B. Min, A. E. Cestra, C. D. Pack, and H. Zaghouani (1998)
J. Immunol. 161, 106-111
   Abstract »    Full Text »    PDF »
The Role of Tumor Necrosis Factor alpha  in Modulating the Quantity of Peripheral Blood-Derived, Cytokine-Driven Human Dendritic Cells and Its Role in Enhancing the Quality of Dendritic Cell Function in Presenting Soluble Antigens to CD4+ T Cells In Vitro.
B.-g. Chen, Y. Shi, J. D. Smith, D. Choi, J. D. Geiger, and J. J. Mule (1998)
Blood 91, 4652-4661
   Abstract »    Full Text »    PDF »
Presentation of a T Cell Receptor Antagonist Peptide by Immunoglobulins Ablates Activation of  T Cells by a Synthetic Peptide or Proteins Requiring Endocytic Processing.
K. L. Legge, B. Min, N. T. Potter, and H. Zaghouani (1997)
J. Exp. Med. 185, 1043-1054
   Abstract »    Full Text »    PDF »
The antigen-binding domain of a human IgG-anti-F(ab')2 autoantibody.
M. Welschof, P. Terness, S. M. Kipriyanov, D. Stanescu, F. Breitling, H. Dorsam, S. Dubel, M. Little, and G. Opelz (1997)
PNAS 94, 1902-1907
   Abstract »    Full Text »    PDF »
Conditions That Induce Tolerance in Mature CD4+ T Cells.
A. Lanoue, C. Bona, H. von Boehmer, and A. Sarukhan (1997)
J. Exp. Med. 185, 405-414
   Abstract »    Full Text »    PDF »
The principle of delivery of T cell epitopes to antigen-presenting cells applied to peptides from influenza virus, ovalbumin, and hen egg lysozyme: Implications for peptide vaccination.
I. B. Rasmussen, E. Lunde, T. E. Michaelsen, B. Bogen, and I. Sandlie (2001)
PNAS 98, 10296-10301
   Abstract »    Full Text »    PDF »


To Advertise     Find Products

Science. ISSN 0036-8075 (print), 1095-9203 (online)