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Science 8 January 1993:
Vol. 259. no. 5092, pp. 224 - 227
DOI: 10.1126/science.7678469
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Articles

Science, Vol 259, Issue 5092, 224-227
Copyright © 1993 by American Association for the Advancement of Science

articles

Presentation of a viral T cell epitope expressed in the CDR3 region of a self immunoglobulin molecule

H Zaghouani, R Steinman, R Nonacs, H Shah, W Gerhard, and C Bona

Department of Microbiology, Mount Sinai School of Medicine, New York, NY 10029.

Synthetic peptides corresponding to microbial epitopes stimulate T cell immunity but their immunogenicity is poor and their half-lives are short. A viral epitope inserted into the complementarity-determining region 3 (CDR3) loop of the heavy chain of a self immunoglobulin (Ig) molecule was generated from the Ig context and was presented by I-Ed class II molecules to virus-specific, CD4+ T cells. Chimeric Ig-peptide was presented 100 to 1000 times more efficiently than free synthetic peptide and was able to prime virus-specific T cells in vivo. These features suggest that antigenized Ig can provide an improved and safe vaccine for the presentation of microbial and other peptides.


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