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Science 1 January 1993:
Vol. 259. no. 5091, pp. 94 - 97
DOI: 10.1126/science.8418502
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Articles

Science, Vol 259, Issue 5091, 94-97
Copyright © 1993 by American Association for the Advancement of Science

articles

Treatment and prevention of rat glioblastoma by immunogenic C6 cells expressing antisense insulin-like growth factor I RNA

J Trojan, TR Johnson, SD Rudin, J Ilan, ML Tykocinski, and J Ilan

INSERM, Paris.

Rat C6 glioma cells express insulin-like growth factor I (IGF-I) and form rapidly growing tumors in syngeneic animals. When transfected with an episome-based vector encoding antisense IGF-I complementary DNA, these cells lost tumorigenicity. Subcutaneous injection of IGF-I antisense-transfected C6 cells into rats prevented formation of both subcutaneous tumors and brain tumors induced by nontransfected C6 cells. The antisense-transfected cells also caused regression of established brain glioblastomas when injected at a point distal to the tumor. These antitumor effects result from a glioma-specific immune response involving CD8+ lymphocytes. Antisense blocking of IGF-I expression may reverse a phenotype that allows C6 glioma cells to evade the immune system.


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