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Science 1 January 1993:
Vol. 259. no. 5091, pp. 84 - 87
DOI: 10.1126/science.8418500
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Articles

Science, Vol 259, Issue 5091, 84-87
Copyright © 1993 by American Association for the Advancement of Science

articles

Regulation of the human hsp70 promoter by p53

SN Agoff, J Hou, DI Linzer, and B Wu

Department of Biochemistry, Molecular Biology, and Cell Biology, Northwestern University, Evanston, IL 60208.

The tumor suppressor p53 is a nuclear phosphoprotein with characteristics of a transcription factor. It displays sequence-specific DNA binding, contains a potent transactivation domain, and has been implicated as both a transcriptional activator and a repressor. Transcription of the human hsp70 gene is stimulated by adenovirus E1a protein. This E1a transactivation of the hsp70 promoter is mediated by CCAAT binding factor (CBF). It is demonstrated here that p53 both represses transcription from the human hsp70 promoter and also interacts with CBF. Thus, the repression of the hsp70 promoter by p53 may be mediated by direct protein-protein interaction with CBF. These results suggest that protein-protein interaction between p53 and specific transcription factors may be an additional mechanism by which p53 regulates gene expression.


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J. Biol. Chem. 273, 19786-19791
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Up-regulation of inducible nitric oxide synthase expression in cancer-prone p53 knockout mice.
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Inhibition of cellular proliferation by the Wilms tumor suppressor WT1 requires association with the inducible chaperone Hsp70.
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p53 Regulates Insulin-Like Growth Factor-I (IGF-I) Receptor Expression and IGF-I-Induced Tyrosine Phosphorylation in an Osteosarcoma Cell Line: Interaction between p53 and Sp1.
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Repression of Transcription From the Human T-Cell Leukemia Virus Type I Long Terminal Repeat and Cellular Gene Promoters by Wild-Type p53.
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Repression of Glucocorticoid Receptor Transactivation and DNA Binding of a Glucocorticoid Response Element within the Serum/Glucocorticoid-Inducible Protein Kinase (sgk) Gene Promoter by the p53 Tumor Suppressor Protein.
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SV40 Large T Antigen Transactivates the Human cdc2 Promoter by Inducing a CCAAT Box Binding Factor.
H. Chen, J. Campisi, and R. Padmanabhan (1996)
J. Biol. Chem. 271, 13959-13967
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p53 Stimulates Promoter Activity of the sgk Serum/Glucocorticoid-inducible Serine/Threonine Protein Kinase Gene in Rodent Mammary Epithelial Cells.
A. C. Maiyar, A. J. Huang, P. T. Phu, H. H. Cha, and G. L. Firestone (1996)
J. Biol. Chem. 271, 12414-12422
   Abstract »    Full Text »    PDF »
p53: puzzle and paradigm..
L J Ko and C Prives (1996)
Genes & Dev. 10, 1054-1072
   PDF »
Wild-type p53 transgenic mice exhibit altered differentiation of the ureteric bud and possess small kidneys..
L A Godley, J B Kopp, M Eckhaus, J J Paglino, J Owens, and H E Varmus (1996)
Genes & Dev. 10, 836-850
   Abstract »    PDF »
Human Cytomegalovirus Immediate-Early Protein IE2 Tethers a Transcriptional Repression Domain to p53.
H.-L. Tsai, G.-H. Kou, S.-C. Chen, C.-W. Wu, and Y.-S. Lin (1996)
J. Biol. Chem. 271, 3534-3540
   Abstract »    Full Text »    PDF »
Modulation of Interleukin-6-induced Plasma Protein Secretion in Hepatoma Cells by p53 Species.
L. Wang, R. J. Rayanade, D. Garcia, K. Patel, H. Pan, and P. B. Sehgal (1995)
J. Biol. Chem. 270, 23159-23165
   Abstract »    Full Text »    PDF »
The WT1 gene product stabilizes p53 and inhibits p53-mediated apoptosis..
S Maheswaran, C Englert, P Bennett, G Heinrich, and D A Haber (1995)
Genes & Dev. 9, 2143-2156
   Abstract »    PDF »
p53 and Sp1 Interact and Cooperate in the Tumor Necrosis Factor-induced Transcriptional Activation of the HIV-1 Long Terminal Repeat.
A. Gualberto and A. S. Baldwin , Jr. (1995)
J. Biol. Chem. 270, 19680-19683
   Abstract »    Full Text »    PDF »
Wild Type p53 Stimulates Expression from the Human Multidrug Resistance Promoter in a p53-negative Cell Line.
M. E. Goldsmith, J. M. Gudas, E. Schneider, and K. H. Cowan (1995)
J. Biol. Chem. 270, 1894-1898
   Abstract »    Full Text »    PDF »
Adenovirus E1B oncoprotein tethers a transcriptional repression domain to p53..
P R Yew, X Liu, and A J Berk (1994)
Genes & Dev. 8, 190-202
   Abstract »    PDF »
Clinical Implications of the p53 Tumor-Suppressor Gene.
C. C. Harris and M. Hollstein (1993)
N. Engl. J. Med. 329, 1318-1327
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Cooperative DNA binding of p53 with TFIID (TBP): a possible mechanism for transcriptional activation..
X Chen, G Farmer, H Zhu, R Prywes, and C Prives (1993)
Genes & Dev. 7, 1837-1849
   Abstract »    PDF »
Repression of Human Reduced Folate Carrier Gene Expression by Wild Type p53.
B. C. Ding, J. R. Whetstine, T. L. Witt, J. D. Schuetz, and L. H. Matherly (2001)
J. Biol. Chem. 276, 8713-8719
   Abstract »    Full Text »    PDF »
The Gene Encoding p202, an Interferon-inducible Negative Regulator of the p53 Tumor Suppressor, Is a Target of p53-mediated Transcriptional Repression.
S. D'Souza, H. Xin, S. Walter, and D. Choubey (2001)
J. Biol. Chem. 276, 298-305
   Abstract »    Full Text »    PDF »
The Phosphotyrosyl Phosphatase Activator Gene Is a Novel p53 Target Gene.
V. Janssens, C. Van Hoof, I. De Baere, W. Merlevede, and J. Goris (2000)
J. Biol. Chem. 275, 20488-20495
   Abstract »    Full Text »    PDF »
The Bradykinin Type 2 Receptor Is a Target for p53-mediated Transcriptional Activation.
Z. Saifudeen, H. Du, S. Dipp, and S. S. El-Dahr (2000)
J. Biol. Chem. 275, 15557-15562
   Abstract »    Full Text »    PDF »


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