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Science 11 December 1992:
Vol. 258. no. 5089, pp. 1801 - 1804
DOI: 10.1126/science.1465617
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Articles

Science, Vol 258, Issue 5089, 1801-1804
Copyright © 1992 by American Association for the Advancement of Science

articles

Invariant chain peptides in most HLA-DR molecules of an antigen-processing mutant

A Sette, S Ceman, RT Kubo, K Sakaguchi, E Appella, DF Hunt, TA Davis, H Michel, J Shabanowitz, R Rudersdorf, and al. et

Laboratory of Genetics, University of Wisconsin, Madison 53706.

Class II major histocompatibility complexes bind peptides in an endosome-like compartment. When the class II null cell line 721.174 was transfected with class II DR3 genes, DR molecules were produced in normal amounts. However, the DR molecules were abnormally conformed and unstable because deletion of an antigen-processing gene had impaired intracellular formation of most class II-peptide complexes. Yet, 70 percent of the DR molecules still bore peptides, 80 percent of which were 21- to 24-amino acid fragments of the class II-associated invariant chain. These peptides were rare on DR3 from control cells. Thus, a defect in the main antigen-processing pathway revealed a process in which DR molecules bind long peptides derived from proteins present in the same compartment.


THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES:
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Differential Requirements for Endosomal Reduction in the Presentation of Two H2-Ed-Restricted Epitopes from Influenza Hemagglutinin.
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Presentation by Recycling MHC Class II Molecules of an Influenza Hemagglutinin-Derived Epitope That Is Revealed in the Early Endosome by Acidification.
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Analysis of MHC Class II Antigen Processing by Quantitation of Peptides that Constitute Nested Sets.
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{beta}57-Asp Plays an Essential Role in the Unique SDS Stability of HLA-DQA1*0102/DQB1*0602 {alpha}{beta} Protein Dimer, the Class II MHC Allele Associated with Protection from Insulin-Dependent Diabetes Mellitus.
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Subtle conformational changes induced in major histocompatibility complex class II molecules by binding peptides.
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Distinct Peptide Loading Pathways for MHC Class II Molecules Associated with Alternative Ii Chain Isoforms.
E. K. Bikoff, G. Kenty, and L. Van Kaer (1998)
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Cutting Edge: A Critical, Invariant Chain-Independent Role for H2-M in Antigen Presentation.
K. Swier, D. R. Brown2, J. J. Bird, W. D. Martin, L. V. Kaer, and S. L. Reiner (1998)
J. Immunol. 160, 540-544
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Aberrant Intermolecular Disulfide Bonding in a Mutant HLA-DM Molecule: Implications for Assembly, Maturation, and Function.
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Functionality of major histocompatibility complex class II molecules in mice doubly deficient for invariant chain and H-2M complexes.
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Trimeric Interactions of the Invariant Chain and Its Association with Major Histocompatibility Complex Class II alpha beta Dimers.
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Self-Release of CLIP in Peptide Loading of HLA-DR Molecules.
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Science 270, 1357-1359
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Accumulation of HLA-DM, a regulator of antigen presentation, in MHC class II compartments.
F Sanderson, M. Kleijmeer, A Kelly, D Verwoerd, A Tulp, J. Neefjes, H. Geuze, and J Trowsdale (1994)
Science 266, 1566-1569
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The kinetic basis of peptide exchange catalysis by HLA-DM.
J. A. Zarutskie, R. Busch, Z. Zavala-Ruiz, M. Rushe, E. D. Mellins, and L. J. Stern (2001)
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