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Science 6 November 1992:
Vol. 258. no. 5084, pp. 1004 - 1007
DOI: 10.1126/science.1439793
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Articles

Science, Vol 258, Issue 5084, 1004-1007
Copyright © 1992 by American Association for the Advancement of Science

articles

A surface protease and the invasive character of plague

OA Sodeinde, YV Subrahmanyam, K Stark, T Quan, Y Bao, and JD Goguen

Department of Molecular Genetics and Microbiology, University of Massachusetts Medical School, Worcester 01655.

A 9.5-kilobase plasmid of Yersinia pestis, the causative agent of plague, is required for high virulence when mice are inoculated with the bacterium by subcutaneous injection. Inactivation of the plasmid gene pla, which encodes a surface protease, increased the median lethal dose of the bacteria for mice by a millionfold. Moreover, cloned pla was sufficient to restore segregants lacking the entire pla-bearing plasmid to full virulence. Both pla+ strains injected subcutaneously and pla- mutants injected intravenously reached high titers in liver and spleen of infected mice, whereas pla- mutants injected subcutaneously failed to do so even though they establish a sustained local infection at the injection site. More inflammatory cells accumulated in lesions caused by the pla- mutants than in lesions produced by the pla+ parent. The Pla protease was shown to be a plasminogen activator with unusual kinetic properties. It can also cleave complement C3 at a specific site.


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   Abstract »    Full Text »    PDF »
Roles of the plasminogen activator streptokinase and the plasminogen-associated M protein in an experimental model for streptococcal impetigo.
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Microbiology 148, 3933-3945
   Abstract »    Full Text »    PDF »
Host Defense Proteins of the Male Reproductive Tract.
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   Full Text »    PDF »
Role of Fraction 1 Antigen of Yersinia pestis in Inhibition of Phagocytosis.
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Infect. Immun. 70, 1453-1460
   Abstract »    Full Text »    PDF »
Yersinia pestis pFra Shows Biovar-Specific Differences and Recent Common Ancestry with a Salmonella enterica Serovar Typhi Plasmid.
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RNA expression patterns change dramatically in human neutrophils exposed to bacteria.
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Blood 97, 2457-2468
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Plasminogen Binding and Activation by Mycoplasma fermentans.
A. Yavlovich, A. A.-R. Higazi, and S. Rottem (2001)
Infect. Immun. 69, 1977-1982
   Abstract »    Full Text »    PDF »
Invasion of Epithelial Cells by Yersinia pestis: Evidence for a Y. pestis-Specific Invasin.
C. Cowan, H. A. Jones, Y. H. Kaya, R. D. Perry, and S. C. Straley (2000)
Infect. Immun. 68, 4523-4530
   Abstract »    Full Text »    PDF »
A PhoP-Regulated Outer Membrane Protease of Salmonella enterica Serovar Typhimurium Promotes Resistance to Alpha-Helical Antimicrobial Peptides.
T. Guina, E. C. Yi, H. Wang, M. Hackett, and S. I. Miller (2000)
J. Bacteriol. 182, 4077-4086
   Abstract »    Full Text »
Streptococcal Erythrogenic Toxin B Abrogates Fibronectin-Dependent Internalization of Streptococcus pyogenes by Cultured Mammalian Cells.
M. S. Chaussee, R. L. Cole, and J. P. M. van Putten (2000)
Infect. Immun. 68, 3226-3232
   Abstract »    Full Text »    PDF »
Yersinia pestis, the cause of plague, is a recently emerged clone of Yersinia pseudotuberculosis.
M. Achtman, K. Zurth, G. Morelli, G. Torrea, A. Guiyoule, and E. Carniel (1999)
PNAS 96, 14043-14048
   Abstract »    Full Text »    PDF »
Selective Distribution of a High-Affinity Plasminogen-Binding Site among Group A Streptococci Associated with Impetigo.
M. D. Svensson, U. Sjobring, and D. E. Bessen (1999)
Infect. Immun. 67, 3915-3920
   Abstract »    Full Text »    PDF »
Complete DNA Sequence and Detailed Analysis of the Yersinia pestis KIM5 Plasmid Encoding Murine Toxin and Capsular Antigen.
L. E. Lindler, G. V. Plano, V. Burland, G. F. Mayhew, and F. R. Blattner (1998)
Infect. Immun. 66, 5731-5742
   Abstract »    Full Text »    PDF »
Expression of Plasminogen Activator Pla of Yersinia pestis Enhances Bacterial Attachment to the Mammalian Extracellular Matrix.
K. Lahteenmaki, R. Virkola, A. Saren, L. Emody, and T. K. Korhonen (1998)
Infect. Immun. 66, 5755-5762
   Abstract »    Full Text »    PDF »
Identification of Two Laminin-Binding Fimbriae, the Type 1 Fimbria of Salmonella enterica Serovar Typhimurium and the G Fimbria of Escherichia coli, as Plasminogen Receptors.
M. Kukkonen, S. Saarela, K. Lahteenmaki, U. Hynonen, B. Westerlund-Wikstrom, M. Rhen, and T. K. Korhonen (1998)
Infect. Immun. 66, 4965-4970
   Abstract »    Full Text »    PDF »
YscO of Yersinia pestis Is a Mobile Core Component of the Yop Secretion System.
P. L. Payne and S. C. Straley (1998)
J. Bacteriol. 180, 3882-3890
   Abstract »    Full Text »
Type III Protein Secretion Systems in Bacterial Pathogens of Animals and Plants.
C. J. Hueck (1998)
Microbiol. Mol. Biol. Rev. 62, 379-433
   Abstract »    Full Text »    PDF »
Molecular Co-operation between Protein PAM and Streptokinase for Plasmin Acquisition by Streptococcus pyogenes.
U. Ringdahl, M. Svensson, A. C. Wistedt, T. Renne, R. Kellner, W. Muller-Esterl, and U. Sjobring (1998)
J. Biol. Chem. 273, 6424-6430
   Abstract »    Full Text »    PDF »
Convergent evolution of apolipoprotein(a) in primates and hedgehog.
R. M. Lawn, K. Schwartz, and L. Patthy (1997)
PNAS 94, 11992-11997
   Abstract »    Full Text »    PDF »


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