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Science 30 October 1992:
Vol. 258. no. 5083, pp. 818 - 821
DOI: 10.1126/science.1359641

Articles

Science, Vol 258, Issue 5083, 818-821
Copyright © 1992 by American Association for the Advancement of Science


articles

Comparative genomic hybridization for molecular cytogenetic analysis of solid tumors

A Kallioniemi, OP Kallioniemi, D Sudar, D Rutovitz, JW Gray, F Waldman, and D Pinkel

Department of Laboratory Medicine, University of California, San Francisco 94143.

Comparative genomic hybridization produces a map of DNA sequence copy number as a function of chromosomal location throughout the entire genome. Differentially labeled test DNA and normal reference DNA are hybridized simultaneously to normal chromosome spreads. The hybridization is detected with two different fluorochromes. Regions of gain or loss of DNA sequences, such as deletions, duplications, or amplifications, are seen as changes in the ratio of the intensities of the two fluorochromes along the target chromosomes. Analysis of tumor cell lines and primary bladder tumors identified 16 different regions of amplification, many in loci not previously known to be amplified.


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Genome-Wide Array-Based Comparative Genomic Hybridization of Diffuse Large B-Cell Lymphoma: Comparison between CD5-Positive and CD5-Negative Cases.
H. Tagawa, S. Tsuzuki, R. Suzuki, S. Karnan, A. Ota, Y. Kameoka, M. Suguro, K. Matsuo, M. Yamaguchi, M. Okamoto, et al. (2004)
Cancer Res. 64, 5948-5955
   Abstract »    Full Text »    PDF »
Minimal 16q Genomic Loss Implicates Cadherin-11 in Retinoblastoma.
M. N. Marchong, D. Chen, T. W. Corson, C. Lee, M. Harmandayan, E. Bowles, N. Chen, and B. L. Gallie (2004)
Mol. Cancer Res. 2, 495-503
   Abstract »    Full Text »    PDF »
Comprehensive whole genome array CGH profiling of mantle cell lymphoma model genomes.
R. J. de Leeuw, J. J. Davies, A. Rosenwald, G. Bebb, R. D. Gascoyne, M. J.S. Dyer, L. M. Staudt, J. A. Martinez-Climent, and W. L. Lam (2004)
Hum. Mol. Genet. 13, 1827-1837
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Reliability of comparative genomic hybridization to detect chromosome abnormalities in first polar bodies and metaphase II oocytes.
C. Gutierrez-Mateo, D. Wells, J. Benet, J. F. Sanchez-Garcia, M. G. Bermudez, I. Belil, J. Egozcue, S. Munne, and J. Navarro (2004)
Hum. Reprod. 19, 2118-2125
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High-Resolution Global Profiling of Genomic Alterations with Long Oligonucleotide Microarray.
C. Brennan, Y. Zhang, C. Leo, B. Feng, C. Cauwels, A. J. Aguirre, M. Kim, A. Protopopov, and L. Chin (2004)
Cancer Res. 64, 4744-4748
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Array Comparative Genomic Hybridization Analysis of Colorectal Cancer Cell Lines and Primary Carcinomas.
E. J. Douglas, H. Fiegler, A. Rowan, S. Halford, D. C. Bicknell, W. Bodmer, I. P. M. Tomlinson, and N. P. Carter (2004)
Cancer Res. 64, 4817-4825
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Overexpression of PDZK1 within the 1q12-q22 Amplicon Is Likely To Be Associated with Drug-Resistance Phenotype in Multiple Myeloma.
J. Inoue, T. Otsuki, A. Hirasawa, I. Imoto, Y. Matsuo, S. Shimizu, M. Taniwaki, and J. Inazawa (2004)
Am. J. Pathol. 165, 71-81
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Comparative genomic hybridization and karyotyping of human embryonic stem cells reveals the occurrence of an isodicentric X chromosome after long-term cultivation.
J. Inzunza, S. Sahlen, K. Holmberg, A.-M. Stromberg, H. Teerijoki, E. Blennow, O. Hovatta, and H. Malmgren (2004)
Mol. Hum. Reprod. 10, 461-466
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Reanalysis of Cancer Drugs: Old Drugs, New Tricks.
G. F. V. Woude, G. J. Kelloff, R. W. Ruddon, H.-M. Koo, C. C. Sigman, J. C. Barrett, R. W. Day, A. P. Dicker, R. S. Kerbel, D. R. Parkinson, et al. (2004)
Clin. Cancer Res. 10, 3897-3907
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Balanced-PCR amplification allows unbiased identification of genomic copy changes in minute cell and tissue samples.
G. Wang, C. Brennan, M. Rook, J. L. Wolfe, C. Leo, L. Chin, H. Pan, W.-H. Liu, B. Price, and G. M. Makrigiorgos (2004)
Nucleic Acids Res. 32, e76
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A cytogeneticist's perspective on genomic microarrays.
L. G. Shaffer and B. A. Bejjani (2004)
Hum. Reprod. Update 10, 221-226
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